贵州农业科学
貴州農業科學
귀주농업과학
GUIZHOU AGRICULTURAL SCIENCES
2009年
12期
1-4
,共4页
玉米%SSR标记%多重PCR
玉米%SSR標記%多重PCR
옥미%SSR표기%다중PCR
maize%SSR marker%multiple PCR
以玉米自交系4011为试验材料,选用玉米SSR核心引物20对,利用单重SSR分子标记技术体系,采用逐步递加引物对的方法,尝试了二重、三重、四重、五重、六重PCR试验.结果筛选出了6个适宜六重PCR扩增的SSR标记组合:phi053/umc2084/umc1196/bnlg1154/mmc0151/bnlg1025、umc1231/umc1196/phi053/umc1222/mmc0151/bnlg1025、umc1231/umc1196/phi053/umc1222/bnlg240/bnlg1025、umc1231/umc1196/phi053/umc1222/1154/bnlg249、bnlg1451/umc1196/phi053/umc1019/phi072/bnlg1025、bnlg1451/umc1196/phi053/umc1545/bnlg2291/bnlg1025.这些标记组合的PCR扩增和PAGE电泳检测良好,从而可提高SSR标记检测的效率,并且降低试验成本.
以玉米自交繫4011為試驗材料,選用玉米SSR覈心引物20對,利用單重SSR分子標記技術體繫,採用逐步遞加引物對的方法,嘗試瞭二重、三重、四重、五重、六重PCR試驗.結果篩選齣瞭6箇適宜六重PCR擴增的SSR標記組閤:phi053/umc2084/umc1196/bnlg1154/mmc0151/bnlg1025、umc1231/umc1196/phi053/umc1222/mmc0151/bnlg1025、umc1231/umc1196/phi053/umc1222/bnlg240/bnlg1025、umc1231/umc1196/phi053/umc1222/1154/bnlg249、bnlg1451/umc1196/phi053/umc1019/phi072/bnlg1025、bnlg1451/umc1196/phi053/umc1545/bnlg2291/bnlg1025.這些標記組閤的PCR擴增和PAGE電泳檢測良好,從而可提高SSR標記檢測的效率,併且降低試驗成本.
이옥미자교계4011위시험재료,선용옥미SSR핵심인물20대,이용단중SSR분자표기기술체계,채용축보체가인물대적방법,상시료이중、삼중、사중、오중、륙중PCR시험.결과사선출료6개괄의륙중PCR확증적SSR표기조합:phi053/umc2084/umc1196/bnlg1154/mmc0151/bnlg1025、umc1231/umc1196/phi053/umc1222/mmc0151/bnlg1025、umc1231/umc1196/phi053/umc1222/bnlg240/bnlg1025、umc1231/umc1196/phi053/umc1222/1154/bnlg249、bnlg1451/umc1196/phi053/umc1019/phi072/bnlg1025、bnlg1451/umc1196/phi053/umc1545/bnlg2291/bnlg1025.저사표기조합적PCR확증화PAGE전영검측량호,종이가제고SSR표기검측적효솔,병차강저시험성본.
4011, a maize inbred line, was tested by using 20 pairs of core maize SSR primers based on the single SSR marker technique to test the two, three, four, five and six fold PCR amplification technique. Six SSR marker combinations of phi053/umc2084/umc1196/bnlg1154/mmc0151/bnlg1025, umc1231/umc1196/phi053/umc1222/mmc0151/bnlg1025, umc1231/umc1196/phi053/umc1222/bnlg240/bnlg1025, umc1231/umc1196/phi053/umc1222/1154/bnlg249, bnlg1451/umc1196/phi053/umc1019/phi072/bnlg1025 and bnlg1451/umc1196/phi053/umc1545/bnlg2291/bnlg1025 to be suitable to six-fold PCR amplification were selected.These marker combinations with good PCR amplification and PAGE lectrophoretic examination can improve the examination efficiency of SSR marker nd reduce the test cost effectively.
