CAJ | 학술논문

通过蛋白质组学技术筛选胃癌相关标志物是目前胃癌研究的热点,也是早期诊断的关键.针对组织蛋白质提取物非常复杂的特点,并根据疏水性的差异,采用反相液相色谱对正常及癌症组织提取蛋白质进行分离.通过比较正常及癌症组织提取蛋白质的谱图差异,收集并酶解差异最大的保留时间为45～47 min的馏分,采用液相色谱-多级质谱联用(LC-MS/MS)鉴定其酶解产物.鉴定结果显示,正常及癌症组织中的共有蛋白质为9个,正常组织中有6个特异蛋白质,而癌症组织中有17个特异蛋白质.通过进一步分析,筛选出胃癌组织中含有的丰度较高的两个蛋白质.应用生物信息学方法分析这些蛋白质,能为将来的药物靶点、药物作用通路研究提供更多的信息.
통과단백질조학기술사선위암상관표지물시목전위암연구적열점,야시조기진단적관건.침대조직단백질제취물비상복잡적특점,병근거소수성적차이,채용반상액상색보대정상급암증조직제취단백질진행분리.통과비교정상급암증조직제취단백질적보도차이,수집병매해차이최대적보류시간위45～47 min적류분,채용액상색보-다급질보련용(LC-MS/MS)감정기매해산물.감정결과현시,정상급암증조직중적공유단백질위9개,정상조직중유6개특이단백질,이암증조직중유17개특이단백질.통과진일보분석,사선출위암조직중함유적봉도교고적량개단백질.응용생물신식학방법분석저사단백질,능위장래적약물파점、약물작용통로연구제공경다적신식.
Screening of tumor markers by proteomic technology is the research focus and key of early diagnosis of stomach cancer study. Aiming at the complexity of the extracted proteins from biological tissue, reversed-phase high performance liquid chromatography (RP-HPLC) was employed as one of the most efficient chromatographic methods. Based on the difference of hydrophobicity, RP-HPLC separation was performed to reduce the complexity of stomach cancer tissue and normal tissue samples, separately. By comparing the chromatograms, different components were collected. The fractions with the retention times from 45 min to 47 min were digested and identified by liquid chromatography-multistage mass spectrometry (LC-MS/MS). Nine common proteins were found in both tumor tissue and normal tissue. Six specific proteins were screened in normal tissue and seventeen specific proteins were found in tumor tissue under the same conditions. Two proteins with higher abundance in tumor tissue were selected for further investigation. These proteins provide more information for future drug target and drug pathway research by the analysis of biological information.