CAJ | 학술논문

双生病毒是一类具有孪生颗粒形态的植物单链病毒,目前双生病毒病害已在多个国家和地区的作物上造成严重危害.近年来,我国多省报道作物上有这类病毒的发生,且有逐年加重和扩散的趋势.根据危害番茄的双生病毒DNA-A序列保守区设计简并引物,对2009年4月采自河北省魏县表现叶片黄化、曲叶症状的4个番茄样品进行PCR检测,均为双生病毒阳性,对样品的扩增片断进行了克隆测序,经序列比对分析,与番茄黄化曲叶病毒(Tomatoleaf curl virus,TYLCV)山东分离物(FJ646611.1)序列相似性为99.25%～99.55%,说明河北番茄黄化曲叶病由TYLCV引起.
쌍생병독시일류구유련생과립형태적식물단련병독,목전쌍생병독병해이재다개국가화지구적작물상조성엄중위해.근년래,아국다성보도작물상유저류병독적발생,차유축년가중화확산적추세.근거위해번가적쌍생병독DNA-A서렬보수구설계간병인물,대2009년4월채자하북성위현표현협편황화、곡협증상적4개번가양품진행PCR검측,균위쌍생병독양성,대양품적확증편단진행료극륭측서,경서렬비대분석,여번가황화곡협병독(Tomatoleaf curl virus,TYLCV)산동분리물(FJ646611.1)서렬상사성위99.25%～99.55%,설명하북번가황화곡협병유TYLCV인기.
Geminiviruses are a group of plant single-stranded viruses characterized by their unique twinned particles,and are currently emerging as a major threat to crop production all over the world.Geminiviruses have been reported in several provinces in China,and disease incidence has been increasing and diffusing in recent years.A pair of degenerate primers Was designed according to the conserved sequence of DNA-A gene of geminivimses infecting tomato,and four tomato samples collected from Weixian county of Hebei Province were found to be mfected by geminiviruses when tested by PCR.The amplified fragments of the two samples were cloned,and sequence analysis indicated that they have 99.25%-99.55%sequence similarity with the tomato leaf curl virus (TYLCV)isolate reported in Shandong(FJ646611.1).The results suggest that the yellow leaf curl disease in Hebei were caused by TYLCV.