CAJ | 학술논문

目的:研究复方黄连制剂(黄连、黄芩、黄柏、栀子和甘草)对华法令和维生素D_3诱发大鼠血管中膜钙化的干预效应.方法:SD大鼠40只,随机分成对照组、钙化组、复方黄连制剂预防组、复方黄连制剂治疗组.钙化组与药物干预组均给予华法令和维生素D_3诱导大鼠血管钙化模型,复方黄连制剂预防组在制作模型前3天给予复方黄连制剂,治疗组则在模型制作完成后给予复方黄连制剂.对照组给予生理盐水.4周后,比较各组Von Kossa染色及血管组织中钙含量,以及血管壁骨保护素(OPG)mRNA和蛋白表达.结果:32只大鼠最终进入结果分析.与对照组比较,钙化组Von Kossa染色显示血管中膜有明显钙沉积,血管组织中钙含量显著增高,分别为(608.32±42.29)μg/g,(1 139.47±230.03)μg/g;血管壁OPGmRNA和蛋白表达则明显减少.预防及治疗应用复方黄连制剂,血管中膜钙化明显改善,血管壁钙含量显著降低,分别为(854.77±12.99)μg/g,(875.78±27.23)μg/g;血管壁OPGmRNA和蛋白也显著增加.与钙化组比较,差异均有统计学意义(P＜0.05).结论:预防或治疗使用复方黄连制剂可能通过增加血管壁骨保护素水平,明显改善血管中膜钙化.
목적:연구복방황련제제(황련、황금、황백、치자화감초)대화법령화유생소D_3유발대서혈관중막개화적간예효응.방법:SD대서40지,수궤분성대조조、개화조、복방황련제제예방조、복방황련제제치료조.개화조여약물간예조균급여화법령화유생소D_3유도대서혈관개화모형,복방황련제제예방조재제작모형전3천급여복방황련제제,치료조칙재모형제작완성후급여복방황련제제.대조조급여생리염수.4주후,비교각조Von Kossa염색급혈관조직중개함량,이급혈관벽골보호소(OPG)mRNA화단백표체.결과:32지대서최종진입결과분석.여대조조비교,개화조Von Kossa염색현시혈관중막유명현개침적,혈관조직중개함량현저증고,분별위(608.32±42.29)μg/g,(1 139.47±230.03)μg/g;혈관벽OPGmRNA화단백표체칙명현감소.예방급치료응용복방황련제제,혈관중막개화명현개선,혈관벽개함량현저강저,분별위(854.77±12.99)μg/g,(875.78±27.23)μg/g;혈관벽OPGmRNA화단백야현저증가.여개화조비교,차이균유통계학의의(P＜0.05).결론:예방혹치료사용복방황련제제가능통과증가혈관벽골보호소수평,명현개선혈관중막개화.
AIM:To investigate the effects of Compound Rhizoma Coptidis ( Rhizoma eoptidis,Radix suctellariae,Cortex phellodendri Chinensis,Fructus gardeniae and Radix et Rhizoma glycyrrhizae) on vascular calcification in rats treated with warfarin and vitamin D_3.METHODS: Thirty-two male SD rats were assigned randomly into control group,calcified group,Compound Rhizoma Coptidis prevented group and treated group.The later three groups were treated with warfarin,and subcutaneously injected with vitamin K_1 and vitamin D_3 for one week to induce extensive calcification of the aorta.Compound Rhizoma Coptidis was given before the first warfarin dose in prevented group and the drug was given after the modeling in the treated group.The control group was treated with normal saline.The calcification in the aorta was analyzed and osteoprotegerin (OPG) mRNA and protein were determined using histomorphometry,RT-PCR and immunohistoehemistry after 4 weeks of drug intervention.RESULTS : The results of 32 rats was analyzed compared to the control group,the area of darkly stained regions by Von Kossa staining and the level of calcium content in aorta wall increased significantly[(608.32±42.29) μg/g vs (1 139.47±230.03) μg/g,P ＜0.05].The OPGmRNA and protein decreased in aortic sections.No artery calcification could be detected in Compound Rhizoma Coptidis prevented group and a little artery calcification could be detected in Compound Rhizoma Coptidis treated group.The level of calcium content in aorta wall significantly lower[(854.77±12.99) μg/g,(875.78±27.23 ) μg/g].The expresion of OPGmRNA and the protein significanfly increased in Compound Rhizoma Coptidis prevented and treated groups.CONCLUSION:Compound Rhizo-ma Coptidis may prevent or regress the vegcular calcifiation,that seems dependent on the upregression of aortic OPG levels.