食品科学
食品科學
식품과학
FOOD SCIENCE
2010年
3期
182-184
,共3页
L-半胱氨酸%溶氧%假单胞菌
L-半胱氨痠%溶氧%假單胞菌
L-반광안산%용양%가단포균
L-cysteine%dissolved oxygen%Pseudomonas sp.
假单胞菌(Pseudomonas sp.)TS 1138在含有DL-2-氨基-△~2-噻哗啉-4-羧酸(DL-2-amino-A2-thiazoline-4-carboxylic acid,DL-ATC)的环境中能够转化DL-ATC生成L-半胱氨酸的酶系.通过固定不同溶氧浓度,探索溶氧对假单胞菌TSl 138发酵产酶的影响.摇瓶实验结果表明:溶氧浓度过低,菌体生长缓慢,细胞产酶能力较低;溶氧浓度过高,菌体生长速度快,但小利于细胞产酶.7L罐发酵实验结果表明:在产酶中后期,应根据溶氧浓度变化来调节搅拌转速或空气流量,使溶氧浓度控制在30%以上.
假單胞菌(Pseudomonas sp.)TS 1138在含有DL-2-氨基-△~2-噻嘩啉-4-羧痠(DL-2-amino-A2-thiazoline-4-carboxylic acid,DL-ATC)的環境中能夠轉化DL-ATC生成L-半胱氨痠的酶繫.通過固定不同溶氧濃度,探索溶氧對假單胞菌TSl 138髮酵產酶的影響.搖瓶實驗結果錶明:溶氧濃度過低,菌體生長緩慢,細胞產酶能力較低;溶氧濃度過高,菌體生長速度快,但小利于細胞產酶.7L罐髮酵實驗結果錶明:在產酶中後期,應根據溶氧濃度變化來調節攪拌轉速或空氣流量,使溶氧濃度控製在30%以上.
가단포균(Pseudomonas sp.)TS 1138재함유DL-2-안기-△~2-새화람-4-최산(DL-2-amino-A2-thiazoline-4-carboxylic acid,DL-ATC)적배경중능구전화DL-ATC생성L-반광안산적매계.통과고정불동용양농도,탐색용양대가단포균TSl 138발효산매적영향.요병실험결과표명:용양농도과저,균체생장완만,세포산매능력교저;용양농도과고,균체생장속도쾌,단소리우세포산매.7L관발효실험결과표명:재산매중후기,응근거용양농도변화래조절교반전속혹공기류량,사용양농도공제재30%이상.
Pseudomonas sp.TS1138 has potential to produce L-cysteine synthetase through asymmetrical hydrolysis of DL-2-alnino-Δ~2-thiazoline-4-carboxylic acid(DL-ATC).The effect of dissolved oxygen level on the production of L-cysteine synthetase was investigated in shake flasks or 7L bioreactor.Results indicated that the cell growth and the production of L-cysteine synthetase were inhibited at low dissolved oxygen level.Although cell growth was improved at the high dissolved oxygen level,the inhibition against production of L-cysteine synthetase was still observed in shake flasks.In 7L bioreactor,dissolved oxygen concentration controlled at more than 30% was helpful for improving the cell growth and the preduetien of L-cysteine synthetase through regulating agitation rate and air flow rate during the middle and late stage.
