中药新药与临床药理
中藥新藥與臨床藥理
중약신약여림상약리
TRADITIONAL CHINESE DRUG RESEARCH&CLINICAL PHARMACOLOGY
2010年
2期
150-152
,共3页
姜黄素%血瘀%脑缺血%全血黏度%能量代谢%脑组织形态
薑黃素%血瘀%腦缺血%全血黏度%能量代謝%腦組織形態
강황소%혈어%뇌결혈%전혈점도%능량대사%뇌조직형태
Curcumine%Blood stasis%Cerebral ischemia%Whole blood viscosity%Energy metabolism%Brain morphology
目的 探讨姜黄素对大鼠血瘀性脑缺血模型影响的特点.方法 采用注射地塞米松复制血瘀模型,姜黄素混悬液给血瘀模型大鼠连续灌服10 d,于第11天给药物后1 h,大鼠两侧颈总动脉结扎30 min,造脑缺血模型;取血测全血黏度;取大鼠脑,一半脑组织用生理盐水制脑匀浆,测脑匀浆ATP水平;另一半脑组织固定、切片,观察脑组织的病理变化.结果 肌肉注射地塞米松可成功复制血瘀模型,结扎双侧颈总动脉可成功复制脑缺血模型.与模型组比较,姜黄素可显著降低血瘀性脑缺血模型全血黏度,显著提高脑匀浆Na~+-K~+-ATP、Mg~(2+)-ATP、Ca~(2+)-ATP和Ca~(2+)-Mg~(2+)-ATP酶活性,显著改善模型所致脑神经细胞和胶质细胞的萎缩.结论 姜黄素可显著改善大鼠血瘀性脑缺血模型全血黏度和脑匀浆ATP酶及脑组织形态.
目的 探討薑黃素對大鼠血瘀性腦缺血模型影響的特點.方法 採用註射地塞米鬆複製血瘀模型,薑黃素混懸液給血瘀模型大鼠連續灌服10 d,于第11天給藥物後1 h,大鼠兩側頸總動脈結扎30 min,造腦缺血模型;取血測全血黏度;取大鼠腦,一半腦組織用生理鹽水製腦勻漿,測腦勻漿ATP水平;另一半腦組織固定、切片,觀察腦組織的病理變化.結果 肌肉註射地塞米鬆可成功複製血瘀模型,結扎雙側頸總動脈可成功複製腦缺血模型.與模型組比較,薑黃素可顯著降低血瘀性腦缺血模型全血黏度,顯著提高腦勻漿Na~+-K~+-ATP、Mg~(2+)-ATP、Ca~(2+)-ATP和Ca~(2+)-Mg~(2+)-ATP酶活性,顯著改善模型所緻腦神經細胞和膠質細胞的萎縮.結論 薑黃素可顯著改善大鼠血瘀性腦缺血模型全血黏度和腦勻漿ATP酶及腦組織形態.
목적 탐토강황소대대서혈어성뇌결혈모형영향적특점.방법 채용주사지새미송복제혈어모형,강황소혼현액급혈어모형대서련속관복10 d,우제11천급약물후1 h,대서량측경총동맥결찰30 min,조뇌결혈모형;취혈측전혈점도;취대서뇌,일반뇌조직용생리염수제뇌균장,측뇌균장ATP수평;령일반뇌조직고정、절편,관찰뇌조직적병리변화.결과 기육주사지새미송가성공복제혈어모형,결찰쌍측경총동맥가성공복제뇌결혈모형.여모형조비교,강황소가현저강저혈어성뇌결혈모형전혈점도,현저제고뇌균장Na~+-K~+-ATP、Mg~(2+)-ATP、Ca~(2+)-ATP화Ca~(2+)-Mg~(2+)-ATP매활성,현저개선모형소치뇌신경세포화효질세포적위축.결론 강황소가현저개선대서혈어성뇌결혈모형전혈점도화뇌균장ATP매급뇌조직형태.
Objective To explore the effect of curcumine on rat cerebral ischemia model of blood stasis. Methods The blood-stasis rat model was established by injection of dexamethasone, then was given gastric infusion curcumine suspension for 10 continuous days. One hour after administration on the 11th day, we made ligation of the bilatory common carotid arteries of rats for 30 min to induce cerebral ischemia rat model. The rat blood sample was used for the detection of whole blood viscosity, and the brain was taken out for the observation of cerebral homogenate ATP and the pathological changes of brain tissue. Results The blood -stasis rats model was established successfully by intramuscular injection of dexamethasone, and cerebral ischemia rats model was established successfully by ligation of bilateral common carotid artery. Compared with the model group, curcumine could significantly reduce the whole blood viscosity of blood-stasis rats model of cerebral ischemia, and significantly increase the activity of Na~+-K~+-ATP, Mg~(2+)-ATP, Ca~(2+)-ATP and Ca~(2+)-Mg~(2+)-ATP in brain homogenate, significant relieve the atrophy of nerve cells and glial cells of the model rats. Conclusion Curcumine can significantly improve the whole blood viscosity, ATP activity in the brain homogenate and morphological changes of brain tissue in rats model of blood stasis .
