中华急诊医学杂志
中華急診醫學雜誌
중화급진의학잡지
CHINESE JOURNAL OF EMERGENCY MEDICINE
2010年
5期
488-492
,共5页
张瑞英%王玉梅%王斐%沈景霞%于萍
張瑞英%王玉梅%王斐%瀋景霞%于萍
장서영%왕옥매%왕비%침경하%우평
大鼠%心肌梗死%2型糖尿病%雷米普利%心室重构%心脏彩色多普勒%心脏功能%葡萄糖转运体
大鼠%心肌梗死%2型糖尿病%雷米普利%心室重構%心髒綵色多普勒%心髒功能%葡萄糖轉運體
대서%심기경사%2형당뇨병%뢰미보리%심실중구%심장채색다보륵%심장공능%포도당전운체
Rats%Myocardial infarction%Type 2 diabetes mellitus%Ramipril%Ventricular remodeling%Color Doppler%Cardiac function%GLUT4
目的 观察2型糖尿病合并心肌梗死大鼠室重构及葡萄糖转运体(GLUT4)表达及雷米普利对其影响.方法 46只雄性Wistar大鼠由哈尔滨医科大学第一临床医学院实验动物中心提供.高脂喂养加低剂量链脲佐菌素(STZ,30mg/kg)腹腔注射,血糖值≥16.7 mmol/L为2型糖尿病模型,结扎冠状动脉左前降支制备心肌梗死模型;糖尿病合并心肌梗死大鼠随机(随机数字法)分为安慰剂组(n,=9),雷米普利组(n=10),糖尿病假手术组(n=10);非糖尿病大鼠随机分为心肌梗死组(n=9)及非糖尿病假手术组(n=8).术后6周查心脏超声、测定心室重量指数、心肌梗死面积及左室非梗死区心肌胶原容积分数.取大鼠非梗死区心肌,采用实时定量RT-PCR技术测定GLUT4 mRNA.Western blot法测定GLUT4蛋白含量.用SPSS 13.0统计软件进行统计学分析;组间比较用方差分析,P<0.05为差异具有统计学意义.结果 与糖尿病假手术组和非糖尿病心肌梗死组相比,安慰剂组EF值显著降低[(32±3)%vs.(65±4)%,P<0.01;(32±3)%vs.(38±4)%,P<0.05];E/A比值(P<0.01)、左室非梗死区胶原容积分数(P<0.05)及心室重量指数(P<0.05)升高.与安慰剂组相比,雷米普利组盯值改善明显[(45±3)%vs.(32±3)%,P<0.01],且E/A比值显著降低(P<0.01),左室非梗死区胶原容积分数下降、心室重量指数减少[(3.1±0.2) vs.(4.2±0.4),P<0.01].安慰剂组GLUT4 mRNA及蛋白表达较非糖尿病心肌梗死组低,与糖尿病假手术组相比差异无统计学意义.与安慰剂组相比,雷米普利组GLUT4 rnRNA及蛋白表达升高.结论 雷米普利可防治心室重构并改善心脏收缩及舒张功能;2型糖尿病合并心肌梗死大鼠心肌GLUT4 mRNA和蛋白的表达下降,雷米普利可以改善糖尿病合并心肌梗死大鼠心肌GLUT4 mRNA和蛋白的表达.
目的 觀察2型糖尿病閤併心肌梗死大鼠室重構及葡萄糖轉運體(GLUT4)錶達及雷米普利對其影響.方法 46隻雄性Wistar大鼠由哈爾濱醫科大學第一臨床醫學院實驗動物中心提供.高脂餵養加低劑量鏈脲佐菌素(STZ,30mg/kg)腹腔註射,血糖值≥16.7 mmol/L為2型糖尿病模型,結扎冠狀動脈左前降支製備心肌梗死模型;糖尿病閤併心肌梗死大鼠隨機(隨機數字法)分為安慰劑組(n,=9),雷米普利組(n=10),糖尿病假手術組(n=10);非糖尿病大鼠隨機分為心肌梗死組(n=9)及非糖尿病假手術組(n=8).術後6週查心髒超聲、測定心室重量指數、心肌梗死麵積及左室非梗死區心肌膠原容積分數.取大鼠非梗死區心肌,採用實時定量RT-PCR技術測定GLUT4 mRNA.Western blot法測定GLUT4蛋白含量.用SPSS 13.0統計軟件進行統計學分析;組間比較用方差分析,P<0.05為差異具有統計學意義.結果 與糖尿病假手術組和非糖尿病心肌梗死組相比,安慰劑組EF值顯著降低[(32±3)%vs.(65±4)%,P<0.01;(32±3)%vs.(38±4)%,P<0.05];E/A比值(P<0.01)、左室非梗死區膠原容積分數(P<0.05)及心室重量指數(P<0.05)升高.與安慰劑組相比,雷米普利組盯值改善明顯[(45±3)%vs.(32±3)%,P<0.01],且E/A比值顯著降低(P<0.01),左室非梗死區膠原容積分數下降、心室重量指數減少[(3.1±0.2) vs.(4.2±0.4),P<0.01].安慰劑組GLUT4 mRNA及蛋白錶達較非糖尿病心肌梗死組低,與糖尿病假手術組相比差異無統計學意義.與安慰劑組相比,雷米普利組GLUT4 rnRNA及蛋白錶達升高.結論 雷米普利可防治心室重構併改善心髒收縮及舒張功能;2型糖尿病閤併心肌梗死大鼠心肌GLUT4 mRNA和蛋白的錶達下降,雷米普利可以改善糖尿病閤併心肌梗死大鼠心肌GLUT4 mRNA和蛋白的錶達.
목적 관찰2형당뇨병합병심기경사대서실중구급포도당전운체(GLUT4)표체급뢰미보리대기영향.방법 46지웅성Wistar대서유합이빈의과대학제일림상의학원실험동물중심제공.고지위양가저제량련뇨좌균소(STZ,30mg/kg)복강주사,혈당치≥16.7 mmol/L위2형당뇨병모형,결찰관상동맥좌전강지제비심기경사모형;당뇨병합병심기경사대서수궤(수궤수자법)분위안위제조(n,=9),뢰미보리조(n=10),당뇨병가수술조(n=10);비당뇨병대서수궤분위심기경사조(n=9)급비당뇨병가수술조(n=8).술후6주사심장초성、측정심실중량지수、심기경사면적급좌실비경사구심기효원용적분수.취대서비경사구심기,채용실시정량RT-PCR기술측정GLUT4 mRNA.Western blot법측정GLUT4단백함량.용SPSS 13.0통계연건진행통계학분석;조간비교용방차분석,P<0.05위차이구유통계학의의.결과 여당뇨병가수술조화비당뇨병심기경사조상비,안위제조EF치현저강저[(32±3)%vs.(65±4)%,P<0.01;(32±3)%vs.(38±4)%,P<0.05];E/A비치(P<0.01)、좌실비경사구효원용적분수(P<0.05)급심실중량지수(P<0.05)승고.여안위제조상비,뢰미보리조정치개선명현[(45±3)%vs.(32±3)%,P<0.01],차E/A비치현저강저(P<0.01),좌실비경사구효원용적분수하강、심실중량지수감소[(3.1±0.2) vs.(4.2±0.4),P<0.01].안위제조GLUT4 mRNA급단백표체교비당뇨병심기경사조저,여당뇨병가수술조상비차이무통계학의의.여안위제조상비,뢰미보리조GLUT4 rnRNA급단백표체승고.결론 뢰미보리가방치심실중구병개선심장수축급서장공능;2형당뇨병합병심기경사대서심기GLUT4 mRNA화단백적표체하강,뢰미보리가이개선당뇨병합병심기경사대서심기GLUT4 mRNA화단백적표체.
Objective To investigate ventricular remodeling and glucose transporter 4(GLUT4) expression in rat with type 2 diabetes mellitus(DM) complicated with myocardial infarction (MI), as well as the effects of ramipril on ventricular remodeling and CLUT4 expression. Method Diabetic animal model (DM) was produced by cholesterol-diet and intraperitoneal injedtion of streptozotocin (STZ,30 mg/kg). The successful model was identified based on the blood gulcose value > 6.7 mmol/L. Left anterior descending coronary artery was ligated to produce the model of MI. 46 wistar rats were randomly(random number) divided into 5 groups: DM + MI + placebo group(placebo group, n = 9), DM + MI + ramipril group(ramipril group, n = 10), DM + sham-operation group (DM group, n = 10),non-DM group + MI (MI group, n = 9), and non-DM + sham-operation group (n = 8). After six weeks, echocardiographic studies was performed ; Left ventricular mass index (LVMI), myocardial infarct size and myocardial collagen volume fraction(CVF) of non-infarction area were also detected. GLUT4 mRNA in the myocardium away from infarction region was measured with real-time fluorescent quantitative RT-PCR and the expression of GLUT4 protein was measured with western blot. Analysis of variance was used to compare the groups. P -values< 0.05 were considered to indicate statistically significant differences. Results Compared with Ml group and DM group,EF[(32 ±3)% vs. (65 ±4)%, P <0.01 and (32 ±3)% vs. (38 ±4)%, P <0.05 respectively] significantly decreased,while E/A ratio (P < 0.01), LVMI (P < 0.05) and CVF (P < 0.05) obviously increased in placebo group. The expression of GLUT4 mRNA and protein were similar between placebo group and DM group, however, much lower than those in MI group(P<0.01).In rampiril group, E/A ratio (P < 0.01), LVMI(3.1±0.2 vs.4.2±0.4, P <0.01) and CVF(P < 0.05) significantly decreased compared with placebo group, while EF[(45±3)% vs.(32±3)%, P <0.01] and the expression of GLUT4 mRNA and protein (P < 0.05) increased. Conclusions Rampiril could improve left ventricular hypertrophy and systolic/di-astolic dysfunction, increase the expression of GLUT4 mRNA and protein in diabetic rats complicated with MI.
