中华小儿外科杂志
中華小兒外科雜誌
중화소인외과잡지
CHINESE JOURNAL OF PEDIATRIC SURGERY
2012年
5期
378-381
,共4页
陈杰%蔡威%邬文杰%葛文亮
陳傑%蔡威%鄔文傑%葛文亮
진걸%채위%오문걸%갈문량
短肠综合征%卡巴胆碱反应曲线%平滑肌肌条实验%胰高血糖素多肽-2
短腸綜閤徵%卡巴膽堿反應麯線%平滑肌肌條實驗%胰高血糖素多肽-2
단장종합정%잡파담감반응곡선%평활기기조실험%이고혈당소다태-2
Short bowel syndrome%Concentration-response curves%Smooth muscle strips test%Glucagon like peptide-2
目的 了解短肠综合征大鼠残存小肠自发性节律性收缩运动及卡巴胆碱诱导小肠平滑肌肌条收缩反应的变化趋势,并探讨腹腔注射胰高血糖素多肽-2 (GLP-2)对短肠综合征大鼠残存小肠术后代偿反应的改善作用及其机制.方法 通过外科手术方法建立动物模型,40只SD大鼠随机分成4组分别为假手术组(设为对照组)、SBS1W组、SBS2W组、G-SBS1W组,G-SBS1W组腹腔注射125μg·kg-1·d-1的GLP-2溶液(20μg/ml),每天2次,连续5d.苏木精-伊红(HE)染色研究小肠全壁形态学改变；电镜观察黏膜上皮及平滑肌细胞的超微结构改变；环形平滑肌肌条实验研究其自发性节律性收缩变化规律及卡巴胆碱诱导肌条收缩反应的变化趋势.结果 短肠综合征大鼠术后1周残存小肠肠管显著扩张；HE染色显示SBS1W组大鼠小肠黏膜上皮增生明显,平滑肌层肥厚、平滑肌增生；环形平滑肌肌条实验提示平滑肌自发性节律性收缩紊乱、幅度不规则、频率变慢；短肠综合征大鼠术后1周卡巴胆碱诱导肌条收缩反应明显增强,术后2周后基本恢复假手术组水平；G-SBS1W组术后1周黏膜上皮进一步代偿,而平滑肌肥厚、增生不明显,环形平滑肌肌条实验及卡巴胆碱反应曲线与假手术组相似.结论 短肠综合征大鼠术后1周残存小肠动力发生动力改变,GLP-2能明显改善短肠综合征大鼠残存小肠的形态及功能.
目的 瞭解短腸綜閤徵大鼠殘存小腸自髮性節律性收縮運動及卡巴膽堿誘導小腸平滑肌肌條收縮反應的變化趨勢,併探討腹腔註射胰高血糖素多肽-2 (GLP-2)對短腸綜閤徵大鼠殘存小腸術後代償反應的改善作用及其機製.方法 通過外科手術方法建立動物模型,40隻SD大鼠隨機分成4組分彆為假手術組(設為對照組)、SBS1W組、SBS2W組、G-SBS1W組,G-SBS1W組腹腔註射125μg·kg-1·d-1的GLP-2溶液(20μg/ml),每天2次,連續5d.囌木精-伊紅(HE)染色研究小腸全壁形態學改變；電鏡觀察黏膜上皮及平滑肌細胞的超微結構改變；環形平滑肌肌條實驗研究其自髮性節律性收縮變化規律及卡巴膽堿誘導肌條收縮反應的變化趨勢.結果 短腸綜閤徵大鼠術後1週殘存小腸腸管顯著擴張；HE染色顯示SBS1W組大鼠小腸黏膜上皮增生明顯,平滑肌層肥厚、平滑肌增生；環形平滑肌肌條實驗提示平滑肌自髮性節律性收縮紊亂、幅度不規則、頻率變慢；短腸綜閤徵大鼠術後1週卡巴膽堿誘導肌條收縮反應明顯增彊,術後2週後基本恢複假手術組水平；G-SBS1W組術後1週黏膜上皮進一步代償,而平滑肌肥厚、增生不明顯,環形平滑肌肌條實驗及卡巴膽堿反應麯線與假手術組相似.結論 短腸綜閤徵大鼠術後1週殘存小腸動力髮生動力改變,GLP-2能明顯改善短腸綜閤徵大鼠殘存小腸的形態及功能.
목적 료해단장종합정대서잔존소장자발성절률성수축운동급잡파담감유도소장평활기기조수축반응적변화추세,병탐토복강주사이고혈당소다태-2 (GLP-2)대단장종합정대서잔존소장술후대상반응적개선작용급기궤제.방법 통과외과수술방법건립동물모형,40지SD대서수궤분성4조분별위가수술조(설위대조조)、SBS1W조、SBS2W조、G-SBS1W조,G-SBS1W조복강주사125μg·kg-1·d-1적GLP-2용액(20μg/ml),매천2차,련속5d.소목정-이홍(HE)염색연구소장전벽형태학개변；전경관찰점막상피급평활기세포적초미결구개변；배형평활기기조실험연구기자발성절률성수축변화규률급잡파담감유도기조수축반응적변화추세.결과 단장종합정대서술후1주잔존소장장관현저확장；HE염색현시SBS1W조대서소장점막상피증생명현,평활기층비후、평활기증생；배형평활기기조실험제시평활기자발성절률성수축문란、폭도불규칙、빈솔변만；단장종합정대서술후1주잡파담감유도기조수축반응명현증강,술후2주후기본회복가수술조수평；G-SBS1W조술후1주점막상피진일보대상,이평활기비후、증생불명현,배형평활기기조실험급잡파담감반응곡선여가수술조상사.결론 단장종합정대서술후1주잔존소장동력발생동력개변,GLP-2능명현개선단장종합정대서잔존소장적형태급공능.
Objective In this study,we aim to investigate the spontaneous contractile function of remnant ileum and the effect of carbachol on contractile function in rats after massive small bowel resection andto further study the effects of GLP-2 on the function of remaining bowel in rats with short bowel syndrome(SBS).Methods A model of SBS by surgical induction was designed.28 male Sprague Dawley rat underwent a 75％ proximal SBR or sham operation and divded into four groups (SBS1W; SBS2W; G-SBS1W; Sham).G-SBS1W rats received 125 μg·kg-1·d-1GLP-2 intra-peritoneally twicw daily for 5 days.The remnant ileum was harvested on the day of 7 and 14.Morphological changes were recorded by HE staining (villus height,circumference,and ISM thickness) and TEM (microvillus,ultrastructure of the smooth muscle cells).Contractility was measured by attaching the distal ileum strips to SMUP-E biological signal processing transducers and exposed to varying doses of carbachol.Results Significant expansion of small bowel was observed in rats with SBS.Smooth muscle proliferation was found by HE staining.Muscle strips test indicated that disordered spontaneous rhythmic contraction,with irregular amplitude and slow frequency occurred in intestinal smooth muscle.Carbachol increased the contractile function of remaining bowel at 1week and induced recovery to normal at 2 weeks.In G-SBS1W group,intestinal appearance was similarto sham group.Conclusions Contractile function of remnant ileum changed in rats with SBS1W.Carbachol increased the contractile function after 1week of surgery.Exogenous supplemented GLP-2 could recover and maintain the function of intestinal ileum in rats with SBS.
