CAJ | 학술논문

目的探讨肠源性内毒素(ET)及CD4+ CD25+调节性T细胞(Treg)在重症急性胰腺炎(SAP)肝损伤中的作用.方法雄性Wistar大鼠60只,随机分成假手术组(SO组)和SAP组.逆行性胰胆管注射牛磺胆酸钠造模.分别于术后3、6、12 h留取标本,检测血淀粉酶及丙氨酸转氨酶水平,鲎试剂法测血浆内毒素含量,流式细胞术测外周血Treg百分数,肝脏及胰腺行病理组织学观察,免疫组化法测肝脏组织Foxp3表达,并对以上指标进行相关性分析.结果病理组织学观察及丙氨酸转氨酶水平证实,SAP组肝脏出现病理性形态改变.与SO组相比,SAP各组Treg百分数显著升高(SAP组3、6、12h分别为:2.26％±0.32％、2.36％±0.48％、2.80％±0.35％),差异有统计学意义(P＜0.01).SAP各组ET水平与SO组相比,差异有统计学意义(P＜0.01),与12 h(0.85±0.11)相比,3 h(0.74±0.11)和6 h(0.78±0.07)差异无统计学意义(P＞0.05).SAP各组肝脏Foxp3蛋白表达明显上调,Treg的表达与ET呈正相关(r=0.89,P＜0.01).结论 SAP可致肝损伤,ET与Treg细胞在SAP肝损伤中可能发挥重要作用.
목적 탐토장원성내독소(ET)급CD4+ CD25+조절성T세포(Treg)재중증급성이선염(SAP)간손상중적작용.방법 웅성Wistar대서60지,수궤분성가수술조(SO조)화SAP조.역행성이담관주사우광담산납조모.분별우술후3、6、12 h류취표본,검측혈정분매급병안산전안매수평,후시제법측혈장내독소함량,류식세포술측외주혈Treg백분수,간장급이선행병리조직학관찰,면역조화법측간장조직Foxp3표체,병대이상지표진행상관성분석.결과 병리조직학관찰급병안산전안매수평증실,SAP조간장출현병이성형태개변.여SO조상비,SAP각조Treg백분수현저승고(SAP조3、6、12h분별위:2.26％±0.32％、2.36％±0.48％、2.80％±0.35％),차이유통계학의의(P＜0.01).SAP각조ET수평여SO조상비,차이유통계학의의(P＜0.01),여12 h(0.85±0.11)상비,3 h(0.74±0.11)화6 h(0.78±0.07)차이무통계학의의(P＞0.05).SAP각조간장Foxp3단백표체명현상조,Treg적표체여ET정정상관(r=0.89,P＜0.01).결론 SAP가치간손상,ET여Treg세포재SAP간손상중가능발휘중요작용.
Objective To investigate the role of CD4+ CD25+ regulatory T cells(Treg) in peripheral blood and intestinal endotoxin (ET) in liver injury of rat severe acute pancreatitis (SAP).Methods Sixty male Wistar rats were randomly allocated into sham operation group(SO group) and severe acute pancreatitis group(SAP group).The rats in SAP group recevied the injection of Sodium Taurocholate(NaTc) into their far-end of bile-pancreatic duct and were sacrificed in 3-,6-and 12-hour,serum amylase(AMY) and alanine aminotransferase(ALT) determined by full-automatic biochemistry instrument,limulus reagent method is used to determine ET content in plasma,the proportion of Treg among peripheral lymphocytes was determined by Flow Cytometry(FCM),HE stain is used to observe pathological changes in liver and pancreas,the protein of Foxp3 activity was evaluated by immunohistochemistry staining,and the relationships between these indicators were analyzed using Pearson correlation analysis test.Results Histopathologic examination and the level of ALT revealed the occurrence of pancreatic inflammation and pathological changes of liver in SAP group.The percentage of Treg in SAP groups significantly increased at 3 h(2.26％ ±0.32％),6 h(2.36 ％ ±0.48％)and 12 h(2.80％ ±0.35％) comparing to the SO groups(P＜0.01) ; plasma ET levels compared with the SO group was statistically significant (P＜ 0,01 ),and 12 h (0.85 ± 0.11) compared to,3 h (0.74±0.11) and 6 h (0.78-±-0.07) was no significant difference (P＞0.05).The expression of Foxp3 protein on the livers were upregulated markedly.Pearson correlation analysis teat showed that quantities of Treg were positively proportional to the levels of ET(r=0.89,P＜0.01) after liver injury of SAP.Conclusions SAP may lead to liver injury and the high plasma levels of ET and Treg in peripheral blood may play an important role in liver injury of SAP.