中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2011年
9期
554-557
,共4页
牙本质%纳米渗漏%自酸蚀
牙本質%納米滲漏%自痠蝕
아본질%납미삼루%자산식
Dentin%Nanoleakage%Self-etching
目的 比较4种自酸蚀粘接剂牙本质粘接界面纳米渗漏及微观形态。方法 选取16颗无龋人离体第三磨牙，制备统一的牙本质玷污层，分别用4种自酸蚀粘接剂A( Adper Prompt)、B( iBond)、C(XenoⅢ)及D(SE Bond)按使用说明进行粘接处理，垂直粘接面切成0.9 mmx4.0 mm试件，避光贮存于氨化硝酸银溶液中24 h，经显影固定脱矿后，切成90 nm超薄切片，铀、铅染色后于透射电镜下观察银离子的渗入情况及界面相关结构。结果4种粘接剂形成的混合层及粘接层薄厚不同：粘接剂A、C混合层较B、D厚，粘接层较B、D簿。4种粘接剂粘接界面均有不同程度纳米渗漏：A(45.02 ±9.49)；B(43.97±8.55)；C(27.02±10.86)；D(12.94±2.07)。粘接剂D组纳米渗漏较小，与其他3种粘接剂间差异有统计学意义(P＜0.05)。结论4种自酸蚀粘接剂混合层和粘接层厚度存在差异，粘接界面的纳米渗漏率不同，一步法自酸蚀粘接系统纳米渗漏较两步法系统明显。
目的 比較4種自痠蝕粘接劑牙本質粘接界麵納米滲漏及微觀形態。方法 選取16顆無齲人離體第三磨牙，製備統一的牙本質玷汙層，分彆用4種自痠蝕粘接劑A( Adper Prompt)、B( iBond)、C(XenoⅢ)及D(SE Bond)按使用說明進行粘接處理，垂直粘接麵切成0.9 mmx4.0 mm試件，避光貯存于氨化硝痠銀溶液中24 h，經顯影固定脫礦後，切成90 nm超薄切片，鈾、鉛染色後于透射電鏡下觀察銀離子的滲入情況及界麵相關結構。結果4種粘接劑形成的混閤層及粘接層薄厚不同：粘接劑A、C混閤層較B、D厚，粘接層較B、D簿。4種粘接劑粘接界麵均有不同程度納米滲漏：A(45.02 ±9.49)；B(43.97±8.55)；C(27.02±10.86)；D(12.94±2.07)。粘接劑D組納米滲漏較小，與其他3種粘接劑間差異有統計學意義(P＜0.05)。結論4種自痠蝕粘接劑混閤層和粘接層厚度存在差異，粘接界麵的納米滲漏率不同，一步法自痠蝕粘接繫統納米滲漏較兩步法繫統明顯。
목적 비교4충자산식점접제아본질점접계면납미삼루급미관형태。방법 선취16과무우인리체제삼마아，제비통일적아본질점오층，분별용4충자산식점접제A( Adper Prompt)、B( iBond)、C(XenoⅢ)급D(SE Bond)안사용설명진행점접처리，수직점접면절성0.9 mmx4.0 mm시건，피광저존우안화초산은용액중24 h，경현영고정탈광후，절성90 nm초박절편，유、연염색후우투사전경하관찰은리자적삼입정황급계면상관결구。결과4충점접제형성적혼합층급점접층박후불동：점접제A、C혼합층교B、D후，점접층교B、D부。4충점접제점접계면균유불동정도납미삼루：A(45.02 ±9.49)；B(43.97±8.55)；C(27.02±10.86)；D(12.94±2.07)。점접제D조납미삼루교소，여기타3충점접제간차이유통계학의의(P＜0.05)。결론4충자산식점접제혼합층화점접층후도존재차이，점접계면적납미삼루솔불동，일보법자산식점접계통납미삼루교량보법계통명현。
Objective To evaluate the nanoleakage and ultramorphology of four self-etching adhesives. Methods Sixteen freshly extracted, caries-free human third molars were selected. A flat dentin surface was exposed by removing occlusal enamel. All teeth were randomly divided into four groups acorrding to four different self-etch adhesive: Adper Prompt( A), iBond(B) , Xeno Ⅲ (C) and SE Bond (D). The dentin were bonded with dentin adhesive system according to manufacturer's directions. Composite layers were built up incrementally. The specimens were sectioned longitudinally across the resin-dentin interface into 4. 0 mm × 0. 9 mm sticks and then traced with ammoniacal silver .solution. Epoxy resin-embedded sections were prepared for transmission electron microscope( TEM ) to observe nanoleakage. The images were qualitatively compared by NIH software, and data was analyzed by SPSS. Results Different thickness of hybrid layer and adhesives layer were observed for each adhesive. The hybrid layer of A, C was thicker than that of B, D, and adhesive layer of D was thicker than the others. The extent of nanoleakage varied among different adhesives: A (45.02 ±9.49),B (43.97 ±8.55), C(27.02 ± 10.86), D (12.94 ±2.07). D presented significantly less silver deposition than any of the others did (P ＜ 0. 05 ). Conclusions The thickness of hybrid layer and adhesive layer vary among the four adhesives. The shape and extent of nanoleakage of each adhesive are also different. Two-step system shows less nanoleakage than one-step systems do.
