中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2010年
1期
33-35
,共3页
朱明利%章松平%曹禾谦%梁伟峰%喻剑华%娄国强
硃明利%章鬆平%曹禾謙%樑偉峰%喻劍華%婁國彊
주명리%장송평%조화겸%량위봉%유검화%루국강
手足口病%T淋巴细胞%HLA-DR抗原%抗原,CD95
手足口病%T淋巴細胞%HLA-DR抗原%抗原,CD95
수족구병%T림파세포%HLA-DR항원%항원,CD95
Hand,foot and mouth disease%T- lymphocytes%HLA-DR Antigens%Antigens,CD95
目的 研究手足口病(HFMD)患者外周血淋巴细胞CD95、HLA-DR活化分子表达的变化及其意义.方法 以健康儿童作为对照,采用流式细胞术,检测58例手足口病患者外周血淋巴细胞CD95标志、T细胞亚群及其HLA-DR抗原表达.结果 HFMD患者外周血CD3~+T细胞的百分率(63.82±7.74)%与健康对照组比较差异有统计学意义(P<0.001),CIM~+T细胞的百分率(34.29±7.33)%明显低于正常对照组(P<0.005);HLA-DR~+淋巴细胞(28.30±7.61)%比正常对照组显著增高(P<0.005);CD8~+T细胞表达HLA-DR水平(1.34±1.12)%明显高于对照组(P<0.005);淋巴细胞CD95表达差异无统计学意义(P>0.05).结论 HFMD患者存在细胞免疫功能紊乱和淋巴细胞异常激活现象,T淋巴细胞的激活以CD8~+T细胞为主,其在抗病毒感染中起着重要作用.
目的 研究手足口病(HFMD)患者外週血淋巴細胞CD95、HLA-DR活化分子錶達的變化及其意義.方法 以健康兒童作為對照,採用流式細胞術,檢測58例手足口病患者外週血淋巴細胞CD95標誌、T細胞亞群及其HLA-DR抗原錶達.結果 HFMD患者外週血CD3~+T細胞的百分率(63.82±7.74)%與健康對照組比較差異有統計學意義(P<0.001),CIM~+T細胞的百分率(34.29±7.33)%明顯低于正常對照組(P<0.005);HLA-DR~+淋巴細胞(28.30±7.61)%比正常對照組顯著增高(P<0.005);CD8~+T細胞錶達HLA-DR水平(1.34±1.12)%明顯高于對照組(P<0.005);淋巴細胞CD95錶達差異無統計學意義(P>0.05).結論 HFMD患者存在細胞免疫功能紊亂和淋巴細胞異常激活現象,T淋巴細胞的激活以CD8~+T細胞為主,其在抗病毒感染中起著重要作用.
목적 연구수족구병(HFMD)환자외주혈림파세포CD95、HLA-DR활화분자표체적변화급기의의.방법 이건강인동작위대조,채용류식세포술,검측58례수족구병환자외주혈림파세포CD95표지、T세포아군급기HLA-DR항원표체.결과 HFMD환자외주혈CD3~+T세포적백분솔(63.82±7.74)%여건강대조조비교차이유통계학의의(P<0.001),CIM~+T세포적백분솔(34.29±7.33)%명현저우정상대조조(P<0.005);HLA-DR~+림파세포(28.30±7.61)%비정상대조조현저증고(P<0.005);CD8~+T세포표체HLA-DR수평(1.34±1.12)%명현고우대조조(P<0.005);림파세포CD95표체차이무통계학의의(P>0.05).결론 HFMD환자존재세포면역공능문란화림파세포이상격활현상,T림파세포적격활이CD8~+T세포위주,기재항병독감염중기착중요작용.
Objective To investigate the expression of CD95 and special marker for activation of peripheral blood lymphocytes in patients with hand foot and mouth disease (HFMD) and its significance. Methods Immunofluorescent two-color flow cytometry was used to study the expression of CD95 and HLA-DR on lymphocytes in 58 patients with HFMD and 34 normal controls. Results Expression of CD3~+ T cells was significantly lower in patients (63.82±7.74)% than that in controls (P<0.001), meanwhile the expression of CD4~+ T cells was (34.29±7.33 ) %, significantly lower than that of the controls (P<0.005). The percentage of lymphocytes expressing HLA-DR in patients was(23.77±5.78)%, significantly higher than that of the controls (P<0.005 ). Significant difference was observed in the expression of HLA- DR on CD8~+ T cells in patients(1.34±1.12)% as compared with controls (P<0.005). No significant difference in the expression of CD95 on lympnocytes was observed between patients and the controls (P>0.05). Conclusion The findings support that cellular immunodeficiency exists in patients and that lymphocytes were abnormally activated in the patients. The activation of peripheral blood T lymphocytes in patients mainly involves CD8~+ subset and it may play an important role in the immune response to antiviral infection.
