CAJ | 학술논문

目的探讨麻醉和手术创伤对老年大鼠认知功能及海马突触结构的影响.方法 18月龄SD大鼠56只随机分为3组:对照组(C组,n=8)、麻醉组(A组,n=24)、手术组(O组,n=24),A、O组根据麻醉和手术后1、3和7 d分为3个亚组A1、A3、A7组和O1、O3、O7组,每亚组8只.在相应的时间点用Moms水迷宫测试老年大鼠的认知功能,电镜定量测量海马CA3区多形层的突触结构.结果与C组比较,A1、O1、O3组Morris水迷寓测试潜伏期及游泳距离明显延长(P＜0.05),01、03组通过原平台次数明显减少(P＜0.01);与A1组比较,O1组潜伏期明显延长(P＜0.05),游泳距离无明显差异.与C组比较,O1、O3组海马CA3区多形层突触间隙宽度加大变宽(P＜0.01)、突触后膜致密物厚度变薄(P＜0.01)、突触活性区长度缩短(P＜0.05)、突触界面曲率减小(P＜0.01)、穿孔性突触百分率亦下降,07组恢复正常;各麻醉组突触形态改变差异无统计学意义.结论麻醉和手术创伤可损害老年大鼠的认知功能,而海马突触的可塑性可能是术后认知功能障碍的结构基础.
목적 탐토마취화수술창상대노년대서인지공능급해마돌촉결구적영향.방법 18월령SD대서56지수궤분위3조:대조조(C조,n=8)、마취조(A조,n=24)、수술조(O조,n=24),A、O조근거마취화수술후1、3화7 d분위3개아조A1、A3、A7조화O1、O3、O7조,매아조8지.재상응적시간점용Moms수미궁측시노년대서적인지공능,전경정량측량해마CA3구다형층적돌촉결구.결과 여C조비교,A1、O1、O3조Morris수미우측시잠복기급유영거리명현연장(P＜0.05),01、03조통과원평태차수명현감소(P＜0.01);여A1조비교,O1조잠복기명현연장(P＜0.05),유영거리무명현차이.여C조비교,O1、O3조해마CA3구다형층돌촉간극관도가대변관(P＜0.01)、돌촉후막치밀물후도변박(P＜0.01)、돌촉활성구장도축단(P＜0.05)、돌촉계면곡솔감소(P＜0.01)、천공성돌촉백분솔역하강,07조회복정상;각마취조돌촉형태개변차이무통계학의의.결론 마취화수술창상가손해노년대서적인지공능,이해마돌촉적가소성가능시술후인지공능장애적결구기출.
Objective To study the effects of ketamine anesthesia and surgery on cognition and synaptic structure in hippocampus of senile rats.Methods Fifty-six rats aged 18 monks were randomly divided into 3 groups:Groups C(control group),A(ketsmine 40 mg/kg,i.P.)and O(ketamine anesthesia & splenectomy).Morris water maze test was used to observe cognition at Days 1,3 & 7 after ketamine anesthesia or operation respectively.Accordingly Groups A and O were divided into 3 subgroup,i.e.A1.A3,A7 and O1,O3,O7.The time of Morris water maze test was recorded and the synaptic structure was measured in the polymorphic layer of the rat hippocampal field CA3.Results Compared to Group C.the latency period and swimming distance significantly increased in Groups A1,O1 and O3(P＜0.05),and the numbers passing the original platform decreased significantly in Groups O1 and O3(P＜0.01).The latency period was significantly longer in Group O1 than that in Group A1(P＜0.05)while the swimming distance was unchanged.Compared to Group C,the width of synaptic cleft(P＜0.01)increased,the length and area of postsynaptic densities(P＜0.05 or P＜0.01)as well as synaptic curvature(P＜0.01)decreased in Groups O1 and O3.The percentage of perforated synapses also decreased in Groups O1 and O3.Conclusion Surgical injury can impair cognition of senile rats and the synaptic plasticity might be involved in postoperative cognitive dysfunction.