河北医科大学学报
河北醫科大學學報
하북의과대학학보
JOURNAL OF HEBEI MEDICAL UNIVERSITY
2001年
3期
133-136
,共4页
赵群%李勇%马志学%于跃明%范立侨%刘品一
趙群%李勇%馬誌學%于躍明%範立僑%劉品一
조군%리용%마지학%우약명%범립교%류품일
胃肿瘤/免疫学%细胞粘附%红细胞/分析%细胞死亡%流式细胞术/方法%体外研究
胃腫瘤/免疫學%細胞粘附%紅細胞/分析%細胞死亡%流式細胞術/方法%體外研究
위종류/면역학%세포점부%홍세포/분석%세포사망%류식세포술/방법%체외연구
目的 探讨红细胞免疫作用对胃癌BGC-823细胞凋亡的影响,以及淋巴细胞的协同作用。方法 应用红细胞免疫粘附肿瘤细胞花环试验、流式细胞术及放射免疫等方法 ,采用正常人外周血红细胞及红细胞-淋巴细胞免疫粘附人胃腺癌BGC-823细胞,检测红细胞及红细胞-淋巴细胞免疫粘附胃癌细胞花环率-胃癌细胞凋亡率、凋亡相关基因Fas、Bcl-2以及凋亡相关信号传导物质cAMP、PKC的变化。结果 红细胞组肿瘤红细胞花环率为(28.41±5.01)%,与对照组比较,红细胞组胃癌细胞凋亡率、Fas基因的表达、胃癌细胞内cAMP水平显著升高,蛋白激酶C(protein kinase C,PKC)水平显著降低(P<0.01);与红细胞组相比,红淋混合组总花环率、胃癌细胞凋亡率、胃癌细胞内cAMP水平进一步增高,Bcl-2基因的表达及PKC水平降低更为显著(P<0.01)。结论 正常人红细胞可免疫粘附体外培养的胃癌BGC-823细胞,通过红细胞的免疫作用,尤其是免疫粘附,可增加凋亡相关基因Fas的表达,增高胃癌细胞内cAMP水平,降低PKC水平,从而可能在诱导胃癌细胞凋亡方面起到某种作用;自身淋巴细胞有不同程度的促进和协同作用。
目的 探討紅細胞免疫作用對胃癌BGC-823細胞凋亡的影響,以及淋巴細胞的協同作用。方法 應用紅細胞免疫粘附腫瘤細胞花環試驗、流式細胞術及放射免疫等方法 ,採用正常人外週血紅細胞及紅細胞-淋巴細胞免疫粘附人胃腺癌BGC-823細胞,檢測紅細胞及紅細胞-淋巴細胞免疫粘附胃癌細胞花環率-胃癌細胞凋亡率、凋亡相關基因Fas、Bcl-2以及凋亡相關信號傳導物質cAMP、PKC的變化。結果 紅細胞組腫瘤紅細胞花環率為(28.41±5.01)%,與對照組比較,紅細胞組胃癌細胞凋亡率、Fas基因的錶達、胃癌細胞內cAMP水平顯著升高,蛋白激酶C(protein kinase C,PKC)水平顯著降低(P<0.01);與紅細胞組相比,紅淋混閤組總花環率、胃癌細胞凋亡率、胃癌細胞內cAMP水平進一步增高,Bcl-2基因的錶達及PKC水平降低更為顯著(P<0.01)。結論 正常人紅細胞可免疫粘附體外培養的胃癌BGC-823細胞,通過紅細胞的免疫作用,尤其是免疫粘附,可增加凋亡相關基因Fas的錶達,增高胃癌細胞內cAMP水平,降低PKC水平,從而可能在誘導胃癌細胞凋亡方麵起到某種作用;自身淋巴細胞有不同程度的促進和協同作用。
목적 탐토홍세포면역작용대위암BGC-823세포조망적영향,이급림파세포적협동작용。방법 응용홍세포면역점부종류세포화배시험、류식세포술급방사면역등방법 ,채용정상인외주혈홍세포급홍세포-림파세포면역점부인위선암BGC-823세포,검측홍세포급홍세포-림파세포면역점부위암세포화배솔-위암세포조망솔、조망상관기인Fas、Bcl-2이급조망상관신호전도물질cAMP、PKC적변화。결과 홍세포조종류홍세포화배솔위(28.41±5.01)%,여대조조비교,홍세포조위암세포조망솔、Fas기인적표체、위암세포내cAMP수평현저승고,단백격매C(protein kinase C,PKC)수평현저강저(P<0.01);여홍세포조상비,홍림혼합조총화배솔、위암세포조망솔、위암세포내cAMP수평진일보증고,Bcl-2기인적표체급PKC수평강저경위현저(P<0.01)。결론 정상인홍세포가면역점부체외배양적위암BGC-823세포,통과홍세포적면역작용,우기시면역점부,가증가조망상관기인Fas적표체,증고위암세포내cAMP수평,강저PKC수평,종이가능재유도위암세포조망방면기도모충작용;자신림파세포유불동정도적촉진화협동작용。
Objective To investigate effect of erythrocyte immunization on induction of gastric cancer cell apoptosis and the synergistic action of lymphocytes. Methods Gastric cancer cells with normal erythrocyte, erythrocyte-lymphocyte were cultured in vitro. ATER (associated tumor cell erythrocyte rosette), FCM ( flow cytometry) and RIA (radioimmunoassay) were used to determine percentage of the erythrocyte rosette of erythrocyte group and mixed group, the rate of apoptosis,expression of Fas, Bcl-2 and the level of cAMP, PKC(protein kinase C) in gastric cancer cells. Results The percentage of ATER of erythrocyte group is (28.41 + 5.01 ) %. The expression of Fas, the apoptosis rate increased significantly, the level of cAMP increased significantly, the level of PKC decreased significantly ( P < 0.01 ). In mixed group, the percentage of mixed rosette is (32.28 ±5.37) %, significantly higher than erythrocyte group, the apoptosis rate and the expression of Fasincreased more, the level of cAMP increased more, the Bcl-2 and PKC decreased more ( P <0.01).Conclusion Erythrocytes can adhere BGC- 823 cells cultured in vitro. After immunization, especially immune adhesion, erythrocyte may enhance the expression of Fas, increase the level of cAMP of erythrocyte, decreased the level of PKC, effect the apoptosis of gastric cancer cells. The lymphocyte may has synergistic effect.