CAJ | 학술논문

背景:近年来的研究表明,S100蛋白质类和神经元特异性烯醇化酶的血清浓度与脑损害的程度呈正相关,用于评价脑损伤的程度和预后,但血清S100蛋白质类和磷酸丙酮酸水合酶水平是否可作为评价糖皮质激素对中枢神经系统损伤有治疗作用的指标.目的:观察血清S100蛋白质类和磷酸丙酮酸水合酶变化,探讨甲基强的松龙对大鼠颅脑损伤的治疗作用.设计:随机对照实验.单位:中山大学附属第一医院.材料:实验于2003-09在广州医学院神经研究所完成.选择SD大鼠72只.方法:将72只SD大鼠随机分为3组,正常组8只,对照组和治疗组大鼠各32只,对照组和治疗组分别分为伤后1,6,12,24 h组,每个小组8只.①造模:采用Feeney法造成鼠脑挫裂伤模型后,正常组只行开颅手术,不作头颅打击.②给药及取材:治疗组大鼠致伤后即刻腹腔内注射30 mg/kg甲基强的松龙,对照组则即刻腹腔内注射30 mg/kg生理盐水.正常组不注射药物,开颅后随即断头取血;对照组和治疗组大鼠分别在伤后1,6,12,24 h时间点断头取血,离心后取上清液,用酶联免疫吸附法作血清S100蛋白质类和磷酸丙酮酸水合酶水平检测.主要观察指标:①各组大鼠血清S100蛋白质类水平.②各组大鼠血清磷酸丙酮酸水合酶水平.结果:72只大鼠均进入结果分析.①正常组血清S100蛋白质类(0.35±0.029)μg/L,除伤后24 h治疗组血清S100蛋白质类与正常组无差异外,对照组和治疗组血清S100蛋白质类水平均比正常组高(P＜0.01或P＜0.05);治疗组在伤后6,12 h和24 h比对照组血清S100蛋白质类低(P＜0.01或P＜0.05).②正常组血清磷酸丙酮酸水合酶(8.35±1.01)μg/L,除伤后24 h对照组和治疗组血清磷酸丙酮酸水合酶与正常组无差异外,其余时间点对照组和治疗组血清磷酸丙酮酸水合酶水平均比正常组高(P＜0.01);治疗组在伤后1,6,12 h时间点比对照组血清磷酸丙酮酸水合酶低(P＜0.01或P＜0.05).结论:大鼠颅脑损伤后血清S100蛋白质类和磷酸丙酮酸水合酶可以作为反映脑损伤程度的标志物,甲基强的松龙对损伤的脑组织有治疗作用. 揹景:近年來的研究錶明,S100蛋白質類和神經元特異性烯醇化酶的血清濃度與腦損害的程度呈正相關,用于評價腦損傷的程度和預後,但血清S100蛋白質類和燐痠丙酮痠水閤酶水平是否可作為評價糖皮質激素對中樞神經繫統損傷有治療作用的指標.目的:觀察血清S100蛋白質類和燐痠丙酮痠水閤酶變化,探討甲基彊的鬆龍對大鼠顱腦損傷的治療作用.設計:隨機對照實驗.單位:中山大學附屬第一醫院.材料:實驗于2003-09在廣州醫學院神經研究所完成.選擇SD大鼠72隻.方法:將72隻SD大鼠隨機分為3組,正常組8隻,對照組和治療組大鼠各32隻,對照組和治療組分彆分為傷後1,6,12,24 h組,每箇小組8隻.①造模:採用Feeney法造成鼠腦挫裂傷模型後,正常組隻行開顱手術,不作頭顱打擊.②給藥及取材:治療組大鼠緻傷後即刻腹腔內註射30 mg/kg甲基彊的鬆龍,對照組則即刻腹腔內註射30 mg/kg生理鹽水.正常組不註射藥物,開顱後隨即斷頭取血;對照組和治療組大鼠分彆在傷後1,6,12,24 h時間點斷頭取血,離心後取上清液,用酶聯免疫吸附法作血清S100蛋白質類和燐痠丙酮痠水閤酶水平檢測.主要觀察指標:①各組大鼠血清S100蛋白質類水平.②各組大鼠血清燐痠丙酮痠水閤酶水平.結果:72隻大鼠均進入結果分析.①正常組血清S100蛋白質類(0.35±0.029)μg/L,除傷後24 h治療組血清S100蛋白質類與正常組無差異外,對照組和治療組血清S100蛋白質類水平均比正常組高(P＜0.01或P＜0.05);治療組在傷後6,12 h和24 h比對照組血清S100蛋白質類低(P＜0.01或P＜0.05).②正常組血清燐痠丙酮痠水閤酶(8.35±1.01)μg/L,除傷後24 h對照組和治療組血清燐痠丙酮痠水閤酶與正常組無差異外,其餘時間點對照組和治療組血清燐痠丙酮痠水閤酶水平均比正常組高(P＜0.01);治療組在傷後1,6,12 h時間點比對照組血清燐痠丙酮痠水閤酶低(P＜0.01或P＜0.05).結論:大鼠顱腦損傷後血清S100蛋白質類和燐痠丙酮痠水閤酶可以作為反映腦損傷程度的標誌物,甲基彊的鬆龍對損傷的腦組織有治療作用.
배경:근년래적연구표명,S100단백질류화신경원특이성희순화매적혈청농도여뇌손해적정도정정상관,용우평개뇌손상적정도화예후,단혈청S100단백질류화린산병동산수합매수평시부가작위평개당피질격소대중추신경계통손상유치료작용적지표.목적:관찰혈청S100단백질류화린산병동산수합매변화,탐토갑기강적송룡대대서로뇌손상적치료작용.설계:수궤대조실험.단위:중산대학부속제일의원.재료:실험우2003-09재엄주의학원신경연구소완성.선택SD대서72지.방법:장72지SD대서수궤분위3조,정상조8지,대조조화치료조대서각32지,대조조화치료조분별분위상후1,6,12,24 h조,매개소조8지.①조모:채용Feeney법조성서뇌좌렬상모형후,정상조지행개로수술,불작두로타격.②급약급취재:치료조대서치상후즉각복강내주사30 mg/kg갑기강적송룡,대조조칙즉각복강내주사30 mg/kg생리염수.정상조불주사약물,개로후수즉단두취혈;대조조화치료조대서분별재상후1,6,12,24 h시간점단두취혈,리심후취상청액,용매련면역흡부법작혈청S100단백질류화린산병동산수합매수평검측.주요관찰지표:①각조대서혈청S100단백질류수평.②각조대서혈청린산병동산수합매수평.결과:72지대서균진입결과분석.①정상조혈청S100단백질류(0.35±0.029)μg/L,제상후24 h치료조혈청S100단백질류여정상조무차이외,대조조화치료조혈청S100단백질류수평균비정상조고(P＜0.01혹P＜0.05);치료조재상후6,12 h화24 h비대조조혈청S100단백질류저(P＜0.01혹P＜0.05).②정상조혈청린산병동산수합매(8.35±1.01)μg/L,제상후24 h대조조화치료조혈청린산병동산수합매여정상조무차이외,기여시간점대조조화치료조혈청린산병동산수합매수평균비정상조고(P＜0.01);치료조재상후1,6,12 h시간점비대조조혈청린산병동산수합매저(P＜0.01혹P＜0.05).결론:대서로뇌손상후혈청S100단백질류화린산병동산수합매가이작위반영뇌손상정도적표지물,갑기강적송룡대손상적뇌조직유치료작용.
BACKGROUND: In recent researches the concentration of S100 proteins and neuron-specific enolase have been proved positively correlated with the degree of brain injury, and can be used for assessing the degree and prognosis of brain injury. But whether the level of serum S100 proteins and phosphopyruvate hydratase can predict the role of glucocorticosteroid in the treatment of CNS damage is unclear.OBJECTIVE: To observe the changes of serum S100 proteins and phosphopyruvate hydratase level, aiming to explore the role of methylprednisolone in the treatment of craniocerebral inju ryin rats.DESIGN: Randomized controlled study.SETTING: The First Affiliated Hospital of Sun Yat-sen University.MATERIALS: This study was carried out at the Neurological Research Institute of Guangzhou Medical College in September 2003. Totally 72 SD rats were enrolled in this study.METHODS: Totally 72 SD rats were randomly divided into three groups,namely normal group of 8 rats, comparison group and treatment group of 32 rats respectively, the latter two groups were then subdivided into post-trautablishment: Brain contusion and laceration was made in rats according to Feeney's method, rats in normal group were only subjected to craniotomy group, rats were given intraperitoneal injection of methylprednisolone by dosage of 30 mg/kg instantly after injury, which replace by physical saline of the same dosage in comparison group. Rats in normal group were not given intraperitoneal injection, blood specimen was obtained instantly after put to death by cutting off head; in comparison group and treatment group,blood sample was obtained from rat at post-traumatic 1, 6, 12 and 24 hours and centrifuged for collecting superuatant, the level of serum S100 proteins and phosphopyruvate hydratase was detected by using ELISA method.RESULTS: Totally 72 rats were enrolled in this experiment and all regroup was (0.35±0.029) μg/L. The difference of serum S100 proteins was not obvious between normal group and treatment group at post-traumatic 24 hours, but the level of S100 proteins in treatment group and comparison group was significantly higher than normal group (P ＜ 0.01 or P ＜ 0.05);moreover in contrast with comparison group, the level of S100 proteins was found obviously lower in treatment group at post-traumatic 6, 12 hours and hydratase was (8.35±1.01) μg/L in normal group, the difference of serum phosphopyruvate hydratase level between comparison group and treatment group was higher than normal group, displaying statistical significance at all post-traumatic time points (P ＜ 0.01), except for not obvious difference between comparison group at post-traumatic 24 hours and treatment group.While comparing to comparison group, the level of serum phosphopyruvate hydratase was found obviously lower in treatment group at post-traumatic 1,6 and 12 hours time point (P ＜ 0.01 or P ＜ 0.05).CONCLUSION: The level of serum S100 proteins and phosphopyruvate hydratase can be used as predictors for the degree of brain injury in rats, in addition methylprednisolone might exert therapeutic effect on traumatic brain.