CAJ | 학술논문

目的为了研究在中国云南流行的艾滋病毒I型(HIV=1)流行毒株的亚型类型与变异特征,进行分子流行病学研究,为艾滋病预防控制工作提供参考.方法血浆来源于云南省监测系统中的19例HIV-1感染者.采用逆转录聚合酶链反应(RT-PCR)法,从19例患者血清中扩增HIV-1pol-gag共同拥有区片段并直接进行序列测定,所得序列用BioEdit和Mega软件进行亚型及系统发生树分析.结果 19例标本结果显示,12例为CRF08-BC、3例为CRF07-BC、1例为B型、3例为CRF01-AE型.19例毒株间的基因离散率都比较小,CRF08-BC亚型毒株间的基因离散率为2.45%,CRF07-BE亚型毒株间的基因离散率为2.16%;B亚型毒株为5.00%;CRF01-AE亚型毒株与来自泰国的流行CRF01-AE毒株AB032741、U51189的基因离散率分别为3.12%、3.22%.结论上述结果表明,云南地区CRF-BC重组亚型已成为HIV-1流行病学优势亚型,结果基本上反映了我国HIV-1分子流行病学现状.
목적 위료연구재중국운남류행적애자병독I형(HIV=1)류행독주적아형류형여변이특정,진행분자류행병학연구,위애자병예방공제공작제공삼고.방법 혈장래원우운남성감측계통중적19례HIV-1감염자.채용역전록취합매련반응(RT-PCR)법,종19례환자혈청중확증HIV-1pol-gag공동옹유구편단병직접진행서렬측정,소득서렬용BioEdit화Mega연건진행아형급계통발생수분석.결과 19례표본결과현시,12례위CRF08-BC、3례위CRF07-BC、1례위B형、3례위CRF01-AE형.19례독주간적기인리산솔도비교소,CRF08-BC아형독주간적기인리산솔위2.45%,CRF07-BE아형독주간적기인리산솔위2.16%;B아형독주위5.00%;CRF01-AE아형독주여래자태국적류행CRF01-AE독주AB032741、U51189적기인리산솔분별위3.12%、3.22%.결론 상술결과표명,운남지구CRF-BC중조아형이성위HIV-1류행병학우세아형,결과기본상반영료아국HIV-1분자류행병학현상.
Objective The aim of the research is to investigate the subtype and mutation charac-teristic of epidemic human immunodefieiency virus-type I (HIV-1) in Yunnan Province. The molecular epidemiological research was performed to provide reference for control and prevention of AIDS. Meth-ods Nineteen plasma samples were collected from HIV-1 infectors in Yunnan AIDS Monitoring Sys-tem. The HIV-1 pol-gag common fragment was amplified by using the reverse transcription-PCR(RT-PCR) and sequenced directly. The acquired sequences were then aligned by BioEdit and Mega software to analyze the subtype and the phylogenetic tree was drawn. Resnlts In 19 plasma samples,12 cases were CRF08-BC subtype, 3 cases were CRF07-BC, 1 ease was B subtype and 3 cases wereRF01-AE subtype. The gene divergence rate among CRF08-BC subtype HIV-1 strains was 2.45%,CRF07-BC subtype strains 2. 16%, and B subtype strain 5%. The gene divergence rate between CRF01-AE subtype strains and AB032741 as well as U51189 from Thailand was 3.12% and 3. 22%respectively. Conclusion The research shows that the recombinant CRF-BC in Yunnan region has be-come the predominant HIV-1 strain subtype. The result reflects the status quo of HIV-1 molecular ep-idemiology in China.