CAJ | 학술논문

柱状假丝酵母脂肪酶可以选择性催化S-萘普生甲酯、乙酯和乙氧基乙基酯发生水解，从而对化学合成的混旋萘普生进行拆分，制备具有高光学纯度的S-对映体。利用中等极性大孔吸附树脂HZ-806作为固定化载体，可在酶分子周围营造一个有利于反应的微环境，有效地提高酶的催化活力及解决酶的回收。在中等极性大孔吸附树脂固定化酶填充床反应器中，混旋乙氧基乙基萘普生酯可被连续水解拆分，当流量为72mL/h时，酯的水解率为17%，光学纯度为89.1%。
주상가사효모지방매가이선택성최화S-내보생갑지、을지화을양기을기지발생수해，종이대화학합성적혼선내보생진행탁분，제비구유고광학순도적S-대영체。이용중등겁성대공흡부수지HZ-806작위고정화재체，가재매분자주위영조일개유리우반응적미배경，유효지제고매적최화활력급해결매적회수。재중등겁성대공흡부수지고정화매전충상반응기중，혼선을양기을기내보생지가피련속수해탁분，당류량위72mL/h시，지적수해솔위17%，광학순도위89.1%。
Chemically synthesized racemic naproxen was resolved by biological method and optically pure S-enantiomer was prepared. Lipase from Candida cylindracea was able to catalyze the hydrolysis of methyl, ethyl and ethoxyethanol naproxen ester effectively and enantioselectively. S-enatiomer was obtained by separation from remained ester. As a carrier, HZ-806, the porous adsorption resin with middle polarity can make a micro-circumstance helpful to hydrolysis around enzyme molecules. Enzyme activity was improved and enzyme could be recycled easily with immobilization on HZ-806. Racemic ethoxyethanol naproxen ester could be resolved by hydrolyzing continuously in packed bed reactor with lipase immobilized on HZ-806. S-naproxen with 89.1% optical purity was obtained.