CAJ | 학술논문

目的研究海藻酸纯度对移植微囊及囊内人胚胎甲状旁腺组织功能的影响。方法将纯化及未纯化海藻酸微囊及微囊化人胚胎甲状旁腺组织分4组异种移植于小鼠腹腔内，设立空囊对照组。测定人甲状旁腺素数值，通过测定具有特异性的人甲状旁腺素，以明确微囊内甲状旁腺组织的分泌功能，组织学检测微囊形态。结果移植后2个月纯化组及未纯化组人甲状旁腺组织分泌功能有显著差异（P<0.05），分别为(38.8±13.7) pg/ml和(1.2±0.7) pg/ml，纯化及未纯化海藻酸钠制备空囊移植组，植入1个月时微囊冲洗回收率分别为（72±18）%和（24±13）%，植入2个月时为（65±21）%和0。结论纯化的海藻酸制备微囊具有良好的生物相容性，微囊内甲状旁腺组织发挥功能时间比未纯化海藻酸明显延长。
목적연구해조산순도대이식미낭급낭내인배태갑상방선조직공능적영향。방법장순화급미순화해조산미낭급미낭화인배태갑상방선조직분4조이충이식우소서복강내，설립공낭대조조。측정인갑상방선소수치，통과측정구유특이성적인갑상방선소，이명학미낭내갑상방선조직적분비공능，조직학검측미낭형태。결과이식후2개월순화조급미순화조인갑상방선조직분비공능유현저차이（P<0.05），분별위(38.8±13.7) pg/ml화(1.2±0.7) pg/ml，순화급미순화해조산납제비공낭이식조，식입1개월시미낭충세회수솔분별위（72±18）%화（24±13）%，식입2개월시위（65±21）%화0。결론순화적해조산제비미낭구유량호적생물상용성，미낭내갑상방선조직발휘공능시간비미순화해조산명현연장。
Objective　To study the effect of alginate purity on implanted microcapsules and the function of human fetal parathyroid tissue in microcapsules.Methods　Microcapsules and microencapsulated human fetal parathyroid tissue produced with purified and unpurified alginate were xenografted into the peritoneal of mice which were divided into 4 groups. Transplanted empty microcapsules were established as control. Human parathyroid was measured to identify the secretory function of microcapsulated human parathyroid and the morphological changes of microcapsulated tissue were observed.Results　Two months after transplantation, there are significant differences in secreting function of microencapsulated parathyroid tissue between purified and unpurified groups(P＜0.05),(38.8±13.7) pg/ml and (1.2±0.7) pg/ml respectively. The retrieve rates of purified and unpurified alginate empty microcapsules are （72±18）% and (24±13）%，（65±21）% and 0 respectively after 1 and 2 months of transplantation.Conclusion　Microcapsules produced with purified alginate have good biocompatibility, the survival time of parathyroid tissue in the purified microcapsules was prolonged significantly.