西安交通大学学报（医学版）
西安交通大學學報（醫學版）
서안교통대학학보（의학판）
JOURNAL OF XI'AN JIAOTONG UNIVERSITY(MEDICAL SCIENCES)
2009年
6期
732-734
,共3页
甘精胰岛素%转化生长因子β_1%Ⅲ型胶原蛋白%心肌超微结构%糖尿病
甘精胰島素%轉化生長因子β_1%Ⅲ型膠原蛋白%心肌超微結構%糖尿病
감정이도소%전화생장인자β_1%Ⅲ형효원단백%심기초미결구%당뇨병
insulin glargine%transforming growth factor beta 1 (TGF-β_1)%collagen Ⅲ%ultrastructure of myocardium%diabetes
目的 探讨甘精胰岛素对糖尿病大鼠心肌的保护作用.方法 将雄性Wistar大鼠随机分为正常对照组(NC组)、糖尿病组(DM组)和胰岛素治疗组(DI组),DI组给予甘精胰岛素经腹部皮下注射,治疗5周后称重并计算心脏体重比(H/B);免疫组化法测定各组心肌转化生长因子β_1(TGF-β_1)、Ⅲ型胶原蛋白(collagen Ⅲ)的表达;透射电镜观察心肌超微结构.结果 ①与NC组比较,DM组和DI组的H/B、TGF-β_1、collagen Ⅲ均明显增高(P<0.05);与DM组比较,DI组的H/B、TGF -β_1、collagen Ⅲ均下降(P<0.05).②心肌超微结构:DM组肌原纤维排列紊乱,线粒体增多,常有肿胀变性;而DI组病变轻微.结论 甘精胰岛素降低了心肌组织中TGF-β_1及collagen Ⅲ的表达,延缓了糖尿病大鼠心肌纤维化的进程,改善了心肌超微结构.
目的 探討甘精胰島素對糖尿病大鼠心肌的保護作用.方法 將雄性Wistar大鼠隨機分為正常對照組(NC組)、糖尿病組(DM組)和胰島素治療組(DI組),DI組給予甘精胰島素經腹部皮下註射,治療5週後稱重併計算心髒體重比(H/B);免疫組化法測定各組心肌轉化生長因子β_1(TGF-β_1)、Ⅲ型膠原蛋白(collagen Ⅲ)的錶達;透射電鏡觀察心肌超微結構.結果 ①與NC組比較,DM組和DI組的H/B、TGF-β_1、collagen Ⅲ均明顯增高(P<0.05);與DM組比較,DI組的H/B、TGF -β_1、collagen Ⅲ均下降(P<0.05).②心肌超微結構:DM組肌原纖維排列紊亂,線粒體增多,常有腫脹變性;而DI組病變輕微.結論 甘精胰島素降低瞭心肌組織中TGF-β_1及collagen Ⅲ的錶達,延緩瞭糖尿病大鼠心肌纖維化的進程,改善瞭心肌超微結構.
목적 탐토감정이도소대당뇨병대서심기적보호작용.방법 장웅성Wistar대서수궤분위정상대조조(NC조)、당뇨병조(DM조)화이도소치료조(DI조),DI조급여감정이도소경복부피하주사,치료5주후칭중병계산심장체중비(H/B);면역조화법측정각조심기전화생장인자β_1(TGF-β_1)、Ⅲ형효원단백(collagen Ⅲ)적표체;투사전경관찰심기초미결구.결과 ①여NC조비교,DM조화DI조적H/B、TGF-β_1、collagen Ⅲ균명현증고(P<0.05);여DM조비교,DI조적H/B、TGF -β_1、collagen Ⅲ균하강(P<0.05).②심기초미결구:DM조기원섬유배렬문란,선립체증다,상유종창변성;이DI조병변경미.결론 감정이도소강저료심기조직중TGF-β_1급collagen Ⅲ적표체,연완료당뇨병대서심기섬유화적진정,개선료심기초미결구.
Objective To investigate the protective effect of insulin glargine in myocardium of diabetic rats. Methods Male Wistar rats were randomly divided into normal control group (NC), diabetes mellitus group After 6 weeks, we weighed rats and calculated the heart body weight ratio (H/B), Immunohistochemical technique was used to estimate the expression of transforming growth factor beta 1 (TGF-β_1) and the type-Ⅲ collagen (collagen Ⅲ). Myocardial pathologic changes were observed under expression of TGF-β_1 and collagen Ⅲ of DM group and DI group were significantly higher than those in NC group (P<0.05); the levels of H/B and the expression of TGF-β_1 and collagen Ⅲ of DI group were lower than myofibrils were arranged disorderly, mitochondria increased, with swelling and degeneration, while the changes of myocardial ultrastructure were obviously lightened after treatment with insulin glargine. Conclusion Insulin glargine may partly suppress the increased expression of TGF-β_1 and collagen Ⅲ in myocardial of diabetic rats, and it may decrease significantly the myocardial injury of diabetic rats.
