华中科技大学学报（医学版）
華中科技大學學報（醫學版）
화중과기대학학보（의학판）
ACTA UNIVERSITATIS MEDICINAE TONGJI
2009年
5期
575-580
,共6页
乙酰胆碱酯酶%P物质%血管活性肠肽%一氧化氮合酶%大鼠肠道
乙酰膽堿酯酶%P物質%血管活性腸肽%一氧化氮閤酶%大鼠腸道
을선담감지매%P물질%혈관활성장태%일양화담합매%대서장도
acetylcholinesterase%substance P%vasoactive intestinal polypeptide%nitric oxide synthase%intestinal tract,rat
目的 比较观察大鼠肠道内乙酰胆碱酯酶(acetylcholinesterase,ACHE)、P物质(substance P,SP)、血管活性肠肽(vasoactive intestinal polypeptide,VIP)和一氧化氮合酶(nitric oxide synthase,NOS)4种阳性神经元的分布特征及其相互关系.方法 应用酶组织化学和免疫组织化学技术,分别观察AChE、NOS、SP、VIP阳性神经元在大鼠十二指肠、空肠、回肠、结肠和直肠的分布.结果 4种阳性神经元(胞体和纤维)遍布肠壁各层,其分布状态有相随性和相似性规律,即从十二指肠、空肠到回肠逐渐增多,从结肠到直肠逐渐减少;AChE阳性神经元在肠壁各层都十分丰富,NOS和sP阳性神经元以肠肌丛较为丰富,VIP阳性神经元以黏膜下神经丛和黏膜层较为丰富;分布密度依次为AChE>NOS>VIP>SP.4种神经元彼此有共染(递质共存)现象,肠肌丛内神经元互相有突触样联系.结论 AChE、SP、VIP和NOS阳性神经元胞体及神经纤维在不同肠段的分布密度有明显差异,而肠肌丛内各种神经元在形态学上有密切联系,由此推论在肠动力功能调节上它们可能起协调作用.
目的 比較觀察大鼠腸道內乙酰膽堿酯酶(acetylcholinesterase,ACHE)、P物質(substance P,SP)、血管活性腸肽(vasoactive intestinal polypeptide,VIP)和一氧化氮閤酶(nitric oxide synthase,NOS)4種暘性神經元的分佈特徵及其相互關繫.方法 應用酶組織化學和免疫組織化學技術,分彆觀察AChE、NOS、SP、VIP暘性神經元在大鼠十二指腸、空腸、迴腸、結腸和直腸的分佈.結果 4種暘性神經元(胞體和纖維)遍佈腸壁各層,其分佈狀態有相隨性和相似性規律,即從十二指腸、空腸到迴腸逐漸增多,從結腸到直腸逐漸減少;AChE暘性神經元在腸壁各層都十分豐富,NOS和sP暘性神經元以腸肌叢較為豐富,VIP暘性神經元以黏膜下神經叢和黏膜層較為豐富;分佈密度依次為AChE>NOS>VIP>SP.4種神經元彼此有共染(遞質共存)現象,腸肌叢內神經元互相有突觸樣聯繫.結論 AChE、SP、VIP和NOS暘性神經元胞體及神經纖維在不同腸段的分佈密度有明顯差異,而腸肌叢內各種神經元在形態學上有密切聯繫,由此推論在腸動力功能調節上它們可能起協調作用.
목적 비교관찰대서장도내을선담감지매(acetylcholinesterase,ACHE)、P물질(substance P,SP)、혈관활성장태(vasoactive intestinal polypeptide,VIP)화일양화담합매(nitric oxide synthase,NOS)4충양성신경원적분포특정급기상호관계.방법 응용매조직화학화면역조직화학기술,분별관찰AChE、NOS、SP、VIP양성신경원재대서십이지장、공장、회장、결장화직장적분포.결과 4충양성신경원(포체화섬유)편포장벽각층,기분포상태유상수성화상사성규률,즉종십이지장、공장도회장축점증다,종결장도직장축점감소;AChE양성신경원재장벽각층도십분봉부,NOS화sP양성신경원이장기총교위봉부,VIP양성신경원이점막하신경총화점막층교위봉부;분포밀도의차위AChE>NOS>VIP>SP.4충신경원피차유공염(체질공존)현상,장기총내신경원호상유돌촉양련계.결론 AChE、SP、VIP화NOS양성신경원포체급신경섬유재불동장단적분포밀도유명현차이,이장기총내각충신경원재형태학상유밀절련계,유차추론재장동력공능조절상타문가능기협조작용.
Objective To study the distribution characteristics and relationship of acetylcholinesterase (AChE) .substance P(SP), vasoactive intestinal polypeptide (VIP) and nitric oxide synthase ( NOS)-positive neurons in intestinal tract of rats. Methods The distributions of AChE,SP, VIP and NOS-positive neurons in duodenum,jejunum,ileum,colon and rectum of rats were respectively observed by using enzyme histochemical and immunohistochemical methods. Results Four types of positive neurons distributed throughout in each laminae of intestinal tract of rats,and there was accompanying and resemble distribution regularity. From duodenum,jejunum to ileum,four types of positive neurons showed gradual increase tendency in quantity, while from colon to rectum, they showed gradual decrease tendency in quantity. There were extremely abundant AChE-posi-tive neurons in each laminae of intestinal tract of rats,comparatively rich NOS and SP-positive neurons in Auerbach'myenteric plexus(AMP) ,and comparatively rich VIP-ir positive neurons in the Meissner's submucosal plexus(MSP)and mucosa. Density on the distribution of four types of positive neurons was AChE>NOS>VIP>SP. There was coexistence of four types of neurons, and there were synapse-like contacts in four types of neurons in AMP. Conclusion There were significant differences in the density on the distribution of AChE,SP, VIP and NOS-positive neurons in different segments of intestinal tract of rats,and there was close morphological relationship among four types of neurons in AMP. It was deduced that they exerted synergistic effects in regulating motility of the intestine.
