CAJ | 학술논문

目的探讨以兔红细胞作为抗原建立小鼠体液免疫反应动物模型的最适条件.方法微量溶血酶标仪分光光度法测定血清溶血素水平.结果抗兔红细胞溶血素峰时间为免疫后d6(免疫当天为d0);最适兔红细胞抗原量为200%,每鼠腹腔注射0.2 ml.氢化可的松(25 mg/kg)、环磷酰胺(20mg/kg)可以抑制小鼠抗兔红细胞溶血素的生成;甘露聚糖肽(4 mg/kg)对正常小鼠抗兔红细胞溶血素水平影响不大,但对环磷酰胺免疫抑制小鼠的血清溶血素水平具有明显提高作用.结论以兔红细胞作为抗原建立的小鼠体液免疫反应动物模型具有实验过程简单、兔红细胞来源方便、实验误差小等优点,可用于药物对体液免疫功能影响的研究.
목적 탐토이토홍세포작위항원건립소서체액면역반응동물모형적최괄조건.방법 미량용혈매표의분광광도법측정혈청용혈소수평.결과 항토홍세포용혈소봉시간위면역후d6(면역당천위d0);최괄토홍세포항원량위200%,매서복강주사0.2 ml.경화가적송(25 mg/kg)、배린선알(20mg/kg)가이억제소서항토홍세포용혈소적생성;감로취당태(4 mg/kg)대정상소서항토홍세포용혈소수평영향불대,단대배린선알면역억제소서적혈청용혈소수평구유명현제고작용.결론 이토홍세포작위항원건립적소서체액면역반응동물모형구유실험과정간단、토홍세포래원방편、실험오차소등우점,가용우약물대체액면역공능영향적연구.
Objective To establish a mouse model of humoral immune response by immunization with rabbit red blood cells (RRBCs). Methods The mice were immunized with RRBCs and the serum hemolysin level was measured by micro-hemolysis spectrophotometry. Results The peak time needed for hemolysin production against RRBCs was 6 days after the immunization, and 20% RRBCs in a total volume of 0.2 ml was optimal for intraperitoneal injection. Hydrocortisone (25 mg/kg) and cyclophosphamide (20 mg/kg) inhibited hemolysin produotion. Mannatide (4 mg/kg) produced no significant effect on serum hemolysin level in normal mice, but significantly potentiated hemolysin production in immunosuppressed mice induced by cyclophosphamide (20 mg/kg). Conclusion Intraperitoneal RRBC injection is feasible for establishing mouse models of humoral immune response.