CAJ | 학술논문

以烟台海域引发"绿潮"的浒苔为研究对象,采用PCR技术扩增出浒苔的ITS-1、5.8S rDNA及ITS-2片段,将扩增出的片段纯化后克隆至pGEM-T Easy载体,筛选阳性克隆进行序列测定.结果表明,浒苔的ITS-1序列长度为195 bp,5.8S序列为155 bp,ITS-2序列为181 bp,该序列与浒苔属的多种物种ITS序列具有很高的同源性,在ITS-1区、5.8S rDNA区和ITS-2区仅存在4个转换/颠换位点.结合GenBank注册序列和本研究的结果发现,单纯依靠ITS序列并不能对浒苔属种类进行有效的分类鉴定.
이연태해역인발"록조"적호태위연구대상,채용PCR기술확증출호태적ITS-1、5.8S rDNA급ITS-2편단,장확증출적편단순화후극륭지pGEM-T Easy재체,사선양성극륭진행서렬측정.결과표명,호태적ITS-1서렬장도위195 bp,5.8S서렬위155 bp,ITS-2서렬위181 bp,해서렬여호태속적다충물충ITS서렬구유흔고적동원성,재ITS-1구、5.8S rDNA구화ITS-2구부존재4개전환/전환위점.결합GenBank주책서렬화본연구적결과발현,단순의고ITS서렬병불능대호태속충류진행유효적분류감정.
The internal transcribed spacer and 5.8S rDNA of Enteromorpha prolifera forming green tide around the coast of Yantai were amplified by polymerase chain reaction (PCR) . The resultant PCR product was cloned into pGEM-T Easy vector and subjected to nucleotide sequencing. Sequence analysis revealed that the ITS-1, 5.8 S and ITS-2 rDNA of E. prolifera were of 195 bp, 155 bp and 181 bp in length, respectively. The Enteromorpha prolifera ITS sequence shared significant homology with other reported ITS sequences of Enteromorpha with only one transition site and three transversion sites. These results suggested that analysis of ITS sequence could not effectively distinguish the species of Enteromorpha.