CAJ | 학술논문

[目的]为快速和准确地对鹧鸪茶种质材料进行ISSR分析提供有效引物.[方法]基因组DNA的提取采用改良的CTAB法.利用99个ISSR引物,对来自海南岛境内的10个居群中共20份鹧鸪茶种质材料进行PCR扩增,以筛选出适合于所有鹧鸪茶种质材料进行ISSR分析的有效引物.[结果]从99条供试引物中共筛选出15条多态性丰富、条带清晰且可重复性良好的有效引物.用筛选出的15条引物对66份鹧鸪茶种质材料进行ISSR-PCR扩增,均可获得带型丰富和清晰可辨的DNA指纹图谱;共扩增出286条DNA谱带,其中231条为多态性带,占总扩增带数的80.77%,平均每个引物扩增出19.1条谱带.[结论]所筛选的15条引物可以有效地应用于鹧鸪茶种质资源材料的ISSR分析.
[목적]위쾌속화준학지대자고다충질재료진행ISSR분석제공유효인물.[방법]기인조DNA적제취채용개량적CTAB법.이용99개ISSR인물,대래자해남도경내적10개거군중공20빈자고다충질재료진행PCR확증,이사선출괄합우소유자고다충질재료진행ISSR분석적유효인물.[결과]종99조공시인물중공사선출15조다태성봉부、조대청석차가중복성량호적유효인물.용사선출적15조인물대66빈자고다충질재료진행ISSR-PCR확증,균가획득대형봉부화청석가변적DNA지문도보;공확증출286조DNA보대,기중231조위다태성대,점총확증대수적80.77%,평균매개인물확증출19.1조보대.[결론]소사선적15조인물가이유효지응용우자고다충질자원재료적ISSR분석.
[Objective] The aim was to provide effective primers for the rapid and accurate ISSR analysis of the germplasm materials of Mallotus oblongiolus. [Method] The modified CTAB method was used in the extraction of the genomic DNA. 99 ISSR primers were used in the ISSR-PCR amplification for 20 germplasm materials from 10 populations in Hainan Island, so that some primers, which were suitable to all gerplasm materials of M. oblongiolus,could be selected. [Results] 15 effective primers with characteristics of rich polymorphism, clear bands, and good repeatability were selected from 99 test primers. The 15 primers were used in the ISSR-PCR amplification for 66 germplasm materials of M. oblongiolus. From all of which the abundant and distinct DNA fingerprintings could be obtained. 286 DNA bands were obtained, and of which 231 bands were polymorphic, which amounted to 80.77% of the total amplified bands. And 19.1 bands could be obtained with each primer, averagely. [Conclusion] The selected 15 primers could be effectively applied to ISSR analysis of the germplasm resources of M. oblongiolus.