CAJ | 학술논문

采用自行设计、组装的毛细管电泳光导纤维发光二极管诱导荧光检测装置,建立了一种直接测定免疫球蛋白G(IgG)的方法.以蓝色发光二极管(LED)为荧光检测器的激发光源,荧光素异硫氰酸酯(FITC)为柱前衍生试剂,采用毛细管区带电泳,以20 mmol/L 硼砂缓冲溶液(pH 9.2)为背景电解液进行分离检测.通过对衍生反应条件和电泳分离条件进行优化,确定了最佳实验条件,在该条件下,IgG的线性范围为4.5×10~(-8) ～1.2×10~(-6) g/L,检出限为2.0×10~(-8) g/L.该方法简单、高效、选择性好,无需前处理,可用于人血清中IgG含量的测定.
채용자행설계、조장적모세관전영광도섬유발광이겁관유도형광검측장치,건립료일충직접측정면역구단백G(IgG)적방법.이람색발광이겁관(LED)위형광검측기적격발광원,형광소이류청산지(FITC)위주전연생시제,채용모세관구대전영,이20 mmol/L 붕사완충용액(pH 9.2)위배경전해액진행분리검측.통과대연생반응조건화전영분리조건진행우화,학정료최가실험조건,재해조건하,IgG적선성범위위4.5×10~(-8) ～1.2×10~(-6) g/L,검출한위2.0×10~(-8) g/L.해방법간단、고효、선택성호,무수전처리,가용우인혈청중IgG함량적측정.
A new method for direct determination of IgG without purification was developed by capillary electrophoresis(CE) with optical fiber light-emitting diode(LED) induced fluorescence detection.Fluorescence isothiocyanate(FITC) was selected as precolumn derivatization reagent in capillary zone electrophoresis.The conditions of derivatization and separation were optimized.The result indicated that the best derivatization conditions was used buffer of 10 mmol/L borate,reaction time of 16 h at room temperature,and the best separation conditions was used a background electrolyte solution of 20 mmol/L borate buffer(pH 9.2) and separation voltage of 18 kV.Under the optimal conditions,the calibration curve was linear in the range of 4.5×10~(-8)-1.2×10~(-6) g/L.The limit of detection for IgG was 2.0×10~(-8) g/L.The method showed advantages of simpleness,rapid separation,high sensitivity and without prophase disposal in the system,and could be direct applied in the detection of IgG in human serum sample.