CAJ | 학술논문

目的探讨乌司他丁对放射性肺损伤的治疗作用及层黏连蛋白(LN)和羟脯氨酸(Hyp)在放射性肺损伤中的变化.方法雌性SD大鼠100只随机分为3组:正常对照组(C组)24只,单纯照射组(R组)40只,乌司他丁治疗组(U组)36只.R组及U组动物麻醉后,行直线加速器全胸部照射一次,剂量为25 Gy.U组照射后即按乌司他丁100 000 U/kg,尾静脉注射,而后每天以相同剂量尾静脉注射7 d.C组和R组尾静脉注射等体积生理盐水.于照射后第7、15、30、60、90、150天处死动物,取部分肺组织行HE染色及Masson染色,观察组织学及肺胶原纤维等,使用放射免疫法检测血清中LN水平,碱水解法测定血清中Hyp的含量.结果 U组大鼠生存率(88.9%)明显高于R组大鼠(62.5%).R组大鼠肺部在照射后,早期肺泡明显出现充血和水肿,晚期部分肺泡腔塌陷,可见成纤维细胞构成的成纤维病灶,甚至大面积的肺完全实变;与R组相比,U组中、晚期充血、水肿及肺间隔增宽不明显,少见纤维化病灶及实变.R组血清中LN、Hyp水平随着照射时间逐渐增加,在150 d时达到高峰,分别为(129.70±3.48)ng/ml、(193.70±5.41)ng/ml,这与肺部组织学变化趋势基本一致,U组与R组相比却明显减少(P<0.01).结论乌司他丁能有效治疗放射性肺损伤,为放射性肺损伤的防治提供了一种新的方法.
목적 탐토오사타정대방사성폐손상적치료작용급층점련단백(LN)화간포안산(Hyp)재방사성폐손상중적변화.방법 자성SD대서100지수궤분위3조:정상대조조(C조)24지,단순조사조(R조)40지,오사타정치료조(U조)36지.R조급U조동물마취후,행직선가속기전흉부조사일차,제량위25 Gy.U조조사후즉안오사타정100 000 U/kg,미정맥주사,이후매천이상동제량미정맥주사7 d.C조화R조미정맥주사등체적생리염수.우조사후제7、15、30、60、90、150천처사동물,취부분폐조직행HE염색급Masson염색,관찰조직학급폐효원섬유등,사용방사면역법검측혈청중LN수평,감수해법측정혈청중Hyp적함량.결과 U조대서생존솔(88.9%)명현고우R조대서(62.5%).R조대서폐부재조사후,조기폐포명현출현충혈화수종,만기부분폐포강탑함,가견성섬유세포구성적성섬유병조,심지대면적적폐완전실변;여R조상비,U조중、만기충혈、수종급폐간격증관불명현,소견섬유화병조급실변.R조혈청중LN、Hyp수평수착조사시간축점증가,재150 d시체도고봉,분별위(129.70±3.48)ng/ml、(193.70±5.41)ng/ml,저여폐부조직학변화추세기본일치,U조여R조상비각명현감소(P<0.01).결론 오사타정능유효치료방사성폐손상,위방사성폐손상적방치제공료일충신적방법.
Objective To evaluate the effects of ulinastatin on radiation-induced pulmonary fibrosis in Sprague-Dawley(SD) rats and changes of laminin (LN) and hydroxyproline (Hyp) in radiation-induced pulmonary fibrosis. Methods One hundred female SD rats were randomly divided into three groups:group without irradiation or ulinastatin(group C), group treated with ulinastatin after radiation(group U), group only received thoracic irradiation(group R). Whole lungs of rats in group R and group U were irradiated with a total dose of 25 Gy by linear accelerator. The rats were sacrificed on 7, 15,30, 60, 90,150 days after irradiation. Radioimmunity was used to determine LN. Colorimetric assay was used to detect the protein level of Hyp. Results Hyp and LN levels significantly increased, severe inflammatory reaction and pulmonary fibrosis developed in rats only receiving thoracic irradiation,however,administration of ulinastatin prevented the inflammatory process and pulmonary fibrosis. Conclusions LN and Hyp play an important role in radiation-induced lung fibrosis,which could be prediction of radiation-induced lung fibrosis. Ulinastatin plays a protective and regulative role on pulmonary fibrosis of rats, which may serve as a novel therapeutic strategy.