CAJ | 학술논문

目的对一株临床分离的Klebsiella variicola疑似菌株进行鉴定.方法扩增该临床分离菌株的rpoB、gyrA、mdh、infB、phoE和nifH六个基因序列,并测序,构建进化树,分析该菌与Klebsiella variicola以及肺炎克雷伯菌序列的差异性.结果该菌六个基因的进化树中待测菌株分别与Klebsiella variicola各参比株形成一簇,与肺炎克雷伯菌稳定区分.结论在感染性休克患者血流中分离到1株Klebsiella variicola,其基因进化与健康人所携带的肺炎克雷伯菌显著不同.
목적 대일주림상분리적Klebsiella variicola의사균주진행감정.방법 확증해림상분리균주적rpoB、gyrA、mdh、infB、phoE화nifH륙개기인서렬,병측서,구건진화수,분석해균여Klebsiella variicola이급폐염극뢰백균서렬적차이성.결과 해균륙개기인적진화수중대측균주분별여Klebsiella variicola각삼비주형성일족,여폐염극뢰백균은정구분.결론 재감염성휴극환자혈류중분리도1주Klebsiella variicola,기기인진화여건강인소휴대적폐염극뢰백균현저불동.
Objective To identify a suspected Klebsiella variicola strain isolated from a patient with severe septic shock. Methods Sequences of rpoB, gyrA, mdh, infB, phoE and nifH genes were obtained from the clinical isolated suspected Klebsiella variicola strain, and used for constructing phylogenectic trees. Characteristics of phylogenetic trees were used to distinguish Klebsiella variicola from Klebsiella pneumoniae. Results All target gene sequences from this clinical isolated strain constantly formed a cluster with their counterparts from other Klebsiella variicola strains. These clusters were readily differentiated from Klebsiella pneumoniae clusters. Conclusions The clinical isolated strain belongs to Klebsiella variicola class and can cause septic shock. From genetic perspective, Klebsiella variicola is distinctly different from Klebsiella pneumoniae, most of which can be isolated from normal healthy people.