CAJ | 학술논문

目的探讨乳腺癌患者术前血清血管内皮生长因子C(sVEGF-C)水平、组织中环氧化酶2(COX-2)表达与乳腺癌淋巴道转移的关系.方法采用ELISA法检测68例乳腺癌患者、35例乳腺良性病术前以及12名健康女性sVEGF-C水平.免疫组化SP法检测相应乳腺癌患者组织标本中COX-2表达及淋巴管密度(LMVD);分析sVEGF-C、COX-2及LMVD表达之间的关系.结果 (1)乳腺癌患者术前sVEGF-C水平(49±8)pg/ml显著高于健康女性志愿者(5±3)pg/ml和乳腺良性疾病者(8±4)pg/ml;(2)乳腺癌组织COX-2阳性表达率(44.1%)显著高于乳腺良性疾病(11.4%)(P=0.002);LMVD主要位于癌旁组织,癌巢中少有表达;癌旁组织中LMVD(8.7±4.7)较乳腺良性疾病组织中表达(1.8±1.7)显著增多(P=0.002);(3)在乳腺癌患者sVEGF-C水平增高者中,不但COX-2的表达显著高于sVEGF-C水平正常者COX-2的表达(P=0.024),而且LMVD(8.7±3.9)也显著高于sVEGF-C正常者的LMVD(5.6±3.3)(P=0.039);(4)乳腺癌患者COX-2表达阳性组LMVD(9.5±3.7)明显高于COX-2表达阴性组LMVD(6.0±3.8)(P=0.012);(5)在腋淋巴结转移阳性患者中,sVEGF-C水平、COX-2及LMVD表达均高于腋淋巴结转移阴性组(P值分别为0.025,0.022和0.002).结论 sVEGF-C、COX-2及LMVD在乳腺癌的淋巴道转移中有重要作用,可作为估计乳腺癌有无淋巴结转移的参考指标.
목적 탐토유선암환자술전혈청혈관내피생장인자C(sVEGF-C)수평、조직중배양화매2(COX-2)표체여유선암림파도전이적관계.방법 채용ELISA법검측68례유선암환자、35례유선량성병술전이급12명건강녀성sVEGF-C수평.면역조화SP법검측상응유선암환자조직표본중COX-2표체급림파관밀도(LMVD);분석sVEGF-C、COX-2급LMVD표체지간적관계.결과 (1)유선암환자술전sVEGF-C수평(49±8)pg/ml현저고우건강녀성지원자(5±3)pg/ml화유선량성질병자(8±4)pg/ml;(2)유선암조직COX-2양성표체솔(44.1%)현저고우유선량성질병(11.4%)(P=0.002);LMVD주요위우암방조직,암소중소유표체;암방조직중LMVD(8.7±4.7)교유선량성질병조직중표체(1.8±1.7)현저증다(P=0.002);(3)재유선암환자sVEGF-C수평증고자중,불단COX-2적표체현저고우sVEGF-C수평정상자COX-2적표체(P=0.024),이차LMVD(8.7±3.9)야현저고우sVEGF-C정상자적LMVD(5.6±3.3)(P=0.039);(4)유선암환자COX-2표체양성조LMVD(9.5±3.7)명현고우COX-2표체음성조LMVD(6.0±3.8)(P=0.012);(5)재액림파결전이양성환자중,sVEGF-C수평、COX-2급LMVD표체균고우액림파결전이음성조(P치분별위0.025,0.022화0.002).결론 sVEGF-C、COX-2급LMVD재유선암적림파도전이중유중요작용,가작위고계유선암유무림파결전이적삼고지표.
Objective To investigate the relationship among the serum vascular endothelial growth factor C(sVEGF-C),expression of cyclooxygenase 2(COX-2),and lymph vessel density(LMVD),and to discuss their role in tumor progression and lymphatic metastasis.Methods sVEGF-C level was detected by ELISA in 68 pre-operation breast cancer patients,35 breast benign disease patients,and 12 healthy women.Immunohistochemical method was used to detect the expression of COX-2 and lymphatic vessel endothelial hyaluronidase receptor(LYVE)-1 in the breast cancer tissues and benign disease tissues obtained during operation.Resuits (1)The sVEGF-C level of the pre-operation breast cancer group was (49±8)pg/ml,significantly higher than those of the begin tumor group[(8±4)pg/ml,P=0.045]and the healthy women[(5±3)pg/ml,P=0.003].(2)The COX-2 overexpression positive rate in the breast cancer tissues was 44.1%,significantly higher than that in the begin disease tissues(11.4%,P=0.002).Higher than that in the begin disease tissue;the LMVD overexpression in the breast cancer tissues was mainly located in the para-carcinoma tissue(8.7±4.7),significantly higher than that in the begin disease tissues(1.8±1.7,P=0.002).(3)The COX-2 overexpression rate of the patients with a high sVEGF-C level was significantly higher than that of the patients with a low sVEGF-C level(P=0.024).The LMVD level in the breast cancer of the sVEGF-C high level group was(8.7±3.9),significantly higher than that of the sVEGF-C normal group(5.6 ±3.3,P=0.039).(4)The LMVD in the COX-2 overexpression breast cancer group was 9.5±3.7,significantly higher than that in the COX-2 negative group(6.0±3.8,P=0.012).(5)The sVEGF-C level,COX-2,and LMVD expression in the breast cancer patients with lymphatie metastasis were significantly higher than those in the patients without lymphatic metastasis(P=0.025,0.022,and 0.002 respectively).Conclusion VEGF-C,COX-2,and LMVD play important roles in the lymphatie metastasis of breast cancer.They may be important prognostic factors in evaluating breast cancer lymphatic metastasis.