中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2009年
14期
970-972
,共3页
张丽红%邱毅%王克华%李娟%张梅心%刘静%贾颐舫%吴爱华%张爱东%王磊光
張麗紅%邱毅%王剋華%李娟%張梅心%劉靜%賈頤舫%吳愛華%張愛東%王磊光
장려홍%구의%왕극화%리연%장매심%류정%가이방%오애화%장애동%왕뢰광
不育,男(雄)性%精子%原位缺口末端标记%DNA损伤
不育,男(雄)性%精子%原位缺口末耑標記%DNA損傷
불육,남(웅)성%정자%원위결구말단표기%DNA손상
Infertility,male%Spermatozoa%In situ nick-end labeling%DNA damage
目的 比较精子染色质扩散试验(SCD)和脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL)检测精子DNA完整性.方法 对20名已生育的成年健康男性和32例不育患者的精液用上游法筛选得到活动精子,同时进行SCD和TUNEL.结果 不育组和生育组DNA损伤精子百分率的SCD检测结果分别为(12.8±5.8)%和(7.6±3.3)%,2组比较差异有统计学意义(t=3.576,P=0.001);TUNEL检测结果分别为(11.1±5.1)%和(6.8±2.8)%,2组比较差异有统计学意义(t=3.467,P=0.001).SCD与TUNEL检测结果在不育组(r=0.841,P=0.000)和生育组(r=0.823,P=0.000)均具有明显的线性相关关系.生育组SCD检测结果与TUNEL检测结果比较差异无统计学意义(t=1.996,P=0.060),而不育组SCD检测结果明显高于TUNEL检测结果,差异有统计学意义(t=3.023,P=0.005).结论 精子DNA损伤与男性不育有关.与TUNEL比较,SCD是一种更为准确、简单、廉价的检测精子DNA完整性的方法.
目的 比較精子染色質擴散試驗(SCD)和脫氧覈糖覈苷痠末耑轉移酶介導的缺口末耑標記法(TUNEL)檢測精子DNA完整性.方法 對20名已生育的成年健康男性和32例不育患者的精液用上遊法篩選得到活動精子,同時進行SCD和TUNEL.結果 不育組和生育組DNA損傷精子百分率的SCD檢測結果分彆為(12.8±5.8)%和(7.6±3.3)%,2組比較差異有統計學意義(t=3.576,P=0.001);TUNEL檢測結果分彆為(11.1±5.1)%和(6.8±2.8)%,2組比較差異有統計學意義(t=3.467,P=0.001).SCD與TUNEL檢測結果在不育組(r=0.841,P=0.000)和生育組(r=0.823,P=0.000)均具有明顯的線性相關關繫.生育組SCD檢測結果與TUNEL檢測結果比較差異無統計學意義(t=1.996,P=0.060),而不育組SCD檢測結果明顯高于TUNEL檢測結果,差異有統計學意義(t=3.023,P=0.005).結論 精子DNA損傷與男性不育有關.與TUNEL比較,SCD是一種更為準確、簡單、廉價的檢測精子DNA完整性的方法.
목적 비교정자염색질확산시험(SCD)화탈양핵당핵감산말단전이매개도적결구말단표기법(TUNEL)검측정자DNA완정성.방법 대20명이생육적성년건강남성화32례불육환자적정액용상유법사선득도활동정자,동시진행SCD화TUNEL.결과 불육조화생육조DNA손상정자백분솔적SCD검측결과분별위(12.8±5.8)%화(7.6±3.3)%,2조비교차이유통계학의의(t=3.576,P=0.001);TUNEL검측결과분별위(11.1±5.1)%화(6.8±2.8)%,2조비교차이유통계학의의(t=3.467,P=0.001).SCD여TUNEL검측결과재불육조(r=0.841,P=0.000)화생육조(r=0.823,P=0.000)균구유명현적선성상관관계.생육조SCD검측결과여TUNEL검측결과비교차이무통계학의의(t=1.996,P=0.060),이불육조SCD검측결과명현고우TUNEL검측결과,차이유통계학의의(t=3.023,P=0.005).결론 정자DNA손상여남성불육유관.여TUNEL비교,SCD시일충경위준학、간단、렴개적검측정자DNA완정성적방법.
Objective To compare the effects of sperm chmmatin dispersion(SCD)test and TDTmediated dUTP nick end labeling(TUNEL)assay in assessing the DNA fragmentation in human spem.Methods Motile sperms were isolated from the semen samples obtained from 20 healthy fertile men and 32clinically infertile pailents by swim-up technique,and underwent SCD and TUNEL to analyze the DNA fragmentation.Results The rate of sperm with DNA damage of the infertile pailents was 12.8%±5.8%tested by SCD,significantly higher than that of the healtby fertile men(7.6%±3.3%,t=3.576,P=0.001),and the rate of sperm with DNA damage of the infertih patients wag 11.1%±5.1%tested by TUNEL assay.significantly higher than that of the healthy fertile men(6.8%±2.8%.t=3.467,P=0.001).The proportion of sperm cell wlth abnormal DNA integrity meagured by SCD test wag correlated strongly with that determined by TUNEL for the infertile men(r=0.841.P=0.000)and for the fertile men too(r=0.823.P=0.000).The rote of sperm with DNA damage meagured by SCD were not signitlcandy different from those of TUNEL-positive sperm in fertile men(t=1.996,P=0.060).The rate of sperm with DNA damage meagured by SCD wag significantly higher than that meagured bv TUNEL among infertile patients(t=3.023,P=0.005).Conclusion The presence of sperm DNA dsmage may lead to male infertility.SCD is simpler.cheaper and more reliable than TUNEL in testing the sperm DNA damage.
