CAJ | 학술논문

目的观察玻璃体腔注射鼠神经生长因子(NGF)对早期糖尿病大鼠玻璃体中感光细胞间维生素A类结合蛋白(IRBP)含量的影响.方法 Sprague-Dawley雄性大鼠96只,随机分为正常对照组(A 组)及实验组,分别为24、72只.实验组采用链脲佐菌霉素(STZ)一次性腹腔注射制作糖尿病动物模型,再随机分为糖尿病阳性对照组(B组)、糖尿病玻璃体腔生理盐水注射组(C组)和糖尿病玻璃体腔鼠NGF注射组(D组),每组24只大鼠.A组及B组建模后不给予任何干预;C组注射生理盐水4μl,1次/周;D组注射0.5μg/μl的鼠NGF 4 μl,1次/周.注射后2、4、6、8周,采用酶联免疫吸附测定(ELISA)法检测大鼠玻璃体中IRBP含量;作石蜡切片行苏木精-伊红(HE)染色,光学显微镜观察视网膜结构;作超微切片,透射电子显微镜观察视网膜超微结构.结果注射后2周,A、B、C、D组大鼠玻璃体中IRBP含量比较,差异无统计学意义(F=2.833,P=0.052).注射后4、6、8周,A、B、C、D组大鼠玻璃体中IRBP含量比较,差异均有统计学意义(F=22.252,108.459,105.726;P值均=0.000).A组组内注射后不同时间点大鼠玻璃体中IRBP含量比较,差异无统计学意义(F=1.462,P=0.241);B、C、D组组内注射后不同时间点大鼠玻璃体中IRBP含量比较,差异均有统计学意义(F=150.98,63.519,64.604;P值均=0.000).光学显微镜观察发现,A组大鼠视网膜各层组织层次清楚,排列整齐.注射后2、4周,B、C、D组大鼠视网膜结构较A组无明显改变.注射后8周,B、C、D组大鼠视网膜厚度变薄.透射电子显微镜观察发现,A组大鼠视细胞外节膜盘结构清晰,排列规则.注射后2周,B、C、D组大鼠视细胞外节膜盘结构清晰,排列规则整齐.注射后4周,B、C、D组大鼠视细胞外节膜盘局部模糊不清,间隙略有扩大.注射后8周,B、C组大鼠视细胞外节膜盘局部模糊不清,间隙扩大,部分出现溶解、断裂;D组大鼠视细胞外节膜盘局部模糊不清,间隙扩大,未见明显溶解断裂.结论玻璃体腔注射鼠NGF可以有效稳定糖尿病大鼠早期玻璃体中IRBP的含量. 目的觀察玻璃體腔註射鼠神經生長因子(NGF)對早期糖尿病大鼠玻璃體中感光細胞間維生素A類結閤蛋白(IRBP)含量的影響.方法 Sprague-Dawley雄性大鼠96隻,隨機分為正常對照組(A 組)及實驗組,分彆為24、72隻.實驗組採用鏈脲佐菌黴素(STZ)一次性腹腔註射製作糖尿病動物模型,再隨機分為糖尿病暘性對照組(B組)、糖尿病玻璃體腔生理鹽水註射組(C組)和糖尿病玻璃體腔鼠NGF註射組(D組),每組24隻大鼠.A組及B組建模後不給予任何榦預;C組註射生理鹽水4μl,1次/週;D組註射0.5μg/μl的鼠NGF 4 μl,1次/週.註射後2、4、6、8週,採用酶聯免疫吸附測定(ELISA)法檢測大鼠玻璃體中IRBP含量;作石蠟切片行囌木精-伊紅(HE)染色,光學顯微鏡觀察視網膜結構;作超微切片,透射電子顯微鏡觀察視網膜超微結構.結果註射後2週,A、B、C、D組大鼠玻璃體中IRBP含量比較,差異無統計學意義(F=2.833,P=0.052).註射後4、6、8週,A、B、C、D組大鼠玻璃體中IRBP含量比較,差異均有統計學意義(F=22.252,108.459,105.726;P值均=0.000).A組組內註射後不同時間點大鼠玻璃體中IRBP含量比較,差異無統計學意義(F=1.462,P=0.241);B、C、D組組內註射後不同時間點大鼠玻璃體中IRBP含量比較,差異均有統計學意義(F=150.98,63.519,64.604;P值均=0.000).光學顯微鏡觀察髮現,A組大鼠視網膜各層組織層次清楚,排列整齊.註射後2、4週,B、C、D組大鼠視網膜結構較A組無明顯改變.註射後8週,B、C、D組大鼠視網膜厚度變薄.透射電子顯微鏡觀察髮現,A組大鼠視細胞外節膜盤結構清晰,排列規則.註射後2週,B、C、D組大鼠視細胞外節膜盤結構清晰,排列規則整齊.註射後4週,B、C、D組大鼠視細胞外節膜盤跼部模糊不清,間隙略有擴大.註射後8週,B、C組大鼠視細胞外節膜盤跼部模糊不清,間隙擴大,部分齣現溶解、斷裂;D組大鼠視細胞外節膜盤跼部模糊不清,間隙擴大,未見明顯溶解斷裂.結論玻璃體腔註射鼠NGF可以有效穩定糖尿病大鼠早期玻璃體中IRBP的含量.
목적 관찰파리체강주사서신경생장인자(NGF)대조기당뇨병대서파리체중감광세포간유생소A류결합단백(IRBP)함량적영향.방법 Sprague-Dawley웅성대서96지,수궤분위정상대조조(A 조)급실험조,분별위24、72지.실험조채용련뇨좌균매소(STZ)일차성복강주사제작당뇨병동물모형,재수궤분위당뇨병양성대조조(B조)、당뇨병파리체강생리염수주사조(C조)화당뇨병파리체강서NGF주사조(D조),매조24지대서.A조급B조건모후불급여임하간예;C조주사생리염수4μl,1차/주;D조주사0.5μg/μl적서NGF 4 μl,1차/주.주사후2、4、6、8주,채용매련면역흡부측정(ELISA)법검측대서파리체중IRBP함량;작석사절편행소목정-이홍(HE)염색,광학현미경관찰시망막결구;작초미절편,투사전자현미경관찰시망막초미결구.결과 주사후2주,A、B、C、D조대서파리체중IRBP함량비교,차이무통계학의의(F=2.833,P=0.052).주사후4、6、8주,A、B、C、D조대서파리체중IRBP함량비교,차이균유통계학의의(F=22.252,108.459,105.726;P치균=0.000).A조조내주사후불동시간점대서파리체중IRBP함량비교,차이무통계학의의(F=1.462,P=0.241);B、C、D조조내주사후불동시간점대서파리체중IRBP함량비교,차이균유통계학의의(F=150.98,63.519,64.604;P치균=0.000).광학현미경관찰발현,A조대서시망막각층조직층차청초,배렬정제.주사후2、4주,B、C、D조대서시망막결구교A조무명현개변.주사후8주,B、C、D조대서시망막후도변박.투사전자현미경관찰발현,A조대서시세포외절막반결구청석,배렬규칙.주사후2주,B、C、D조대서시세포외절막반결구청석,배렬규칙정제.주사후4주,B、C、D조대서시세포외절막반국부모호불청,간극략유확대.주사후8주,B、C조대서시세포외절막반국부모호불청,간극확대,부분출현용해、단렬;D조대서시세포외절막반국부모호불청,간극확대,미견명현용해단렬.결론 파리체강주사서NGF가이유효은정당뇨병대서조기파리체중IRBP적함량.
Objective To observe the effect of intravitreal injection of mouse nerve growth factor (NGF)on interphotoreceptor retinoid-binding protein(IRBP)in the vitreous of diabetic rats at early stages.Methods Ninety-six male Sprague Dawley(SD)rats were divided into control group(group A,24 rats)and experimental group(72 rats).The rats in experimental group were induced with streptozotocin injection for diabetic retinopathy model,and then randomly divided into positive control group(group B),normal saline group(group C)and NGF group(group D),24 rats in each group.The rats in the group A and B were not intervened.The rats were received intravitreal injection with 4μl normal saline(group C)or 4 μl(0.5 μg/μ1)NGF(group D).At 2,4,6 and 8 weeks after injection,IRBP levels were detected bv enzvmelinked immunosorbent assay(ELISA);hematoxylin-eosin(HE)staining and light microscope were used to observe the morphological changes of the retina;transmission electron microscope was used to observe the retinal uhrastructure.Results At 2 weeks after injection,there was no significant difference in IRBP expression between group A,B,C and D(F=2.833,P=0.052).At 4,6,8 weeks after injection,the differences of IRBP expression between group A,B,C and D were significant(F=22.252,108.459,105.726;P=0.000).At different time points after injection,there was no significant difference in IRBP expression of group A(F=1.462,P=0.241),but there were significant differences in IRBP expression of group B.C and D(F=150.98,63.519,64.604;P=0.000).Light microscope found that the retinal structure was clear in group A and in group B,C,D at 2,4 weeks after injection;the retinal thickness were thinner in group B,C,D at 8 weeks after injection.Transmission electron microscope displayed that the structure of rod outer segments was clear in group A and in group B,C,D at 2 weeks after injection;partly unclear structure of rod outer segments and slightly enlarged gap were observed in group B,C,D at 4,8Weeks after injection.Conclusion Intravitreal injection with NGF can stabilize the IRBP expression in the vitreous of diabetic rats at early stages effectively.