CAJ | 학술논문

目的利用透射电镜观察不同时间段犬腭骨-上颌骨缝内细胞超微结构的变化,以探索颅面部骨缝组织在张力作用下的变化.方法制作镍钛形状记忆合金(NiTi-SMA)扩张器,并用口腔矫形测力器测定其最大力值为3.5 N.选择8周龄杂种犬45只,随机分为空白对照组、实验对照组、实验组.实验对照组和实验组在全麻下,去除硬腭中部后部骨质,缝合口腔和鼻腔侧黏膜,形成宽约8 mm的腭裂模型.实验组将NiTi-SMA安置在硬腭上,分别于牵张后3、7、14、28、56 d处死动物,并制作电镜标本.透射电镜下观察腭骨-上颌骨缝内细胞的变化过程.结果电镜下腭骨-上颌骨缝牵张,首先表现为组织断裂,渗出,细胞死亡,随后是骨和纤维生成细胞群增殖活跃,成骨细胞和成纤维细胞功能增强,最终恢复正常缝组织结构.结论电镜下可以区分缝细胞的类型和功能状态,提示缝牵张是组织修复与再生并存的过程,张力引发细胞反应特别是成骨反应是导致骨缝增宽的主要因素.
목적 이용투사전경관찰불동시간단견악골-상합골봉내세포초미결구적변화,이탐색로면부골봉조직재장력작용하적변화.방법 제작얼태형상기억합금(NiTi-SMA)확장기,병용구강교형측력기측정기최대력치위3.5 N.선택8주령잡충견45지,수궤분위공백대조조、실험대조조、실험조.실험대조조화실험조재전마하,거제경악중부후부골질,봉합구강화비강측점막,형성관약8 mm적악렬모형.실험조장NiTi-SMA안치재경악상,분별우견장후3、7、14、28、56 d처사동물,병제작전경표본.투사전경하관찰악골-상합골봉내세포적변화과정.결과 전경하악골-상합골봉견장,수선표현위조직단렬,삼출,세포사망,수후시골화섬유생성세포군증식활약,성골세포화성섬유세포공능증강,최종회복정상봉조직결구.결론 전경하가이구분봉세포적류형화공능상태,제시봉견장시조직수복여재생병존적과정,장력인발세포반응특별시성골반응시도치골봉증관적주요인소.
Objective To explore the ultrasturcture of the palate-maxillary sutures under tensile forces by transmission electron microscope (TEM). Methods The Suture expanders were made in NiTi-Shape memory alloy (NiTi-SMA) .The maximum force was 3.5 N. Fourteen 8-month old mongrel dogs were used in the study. and control groups, an 8 mm wide cleft was made by surgery. The suture expanders were luted onto the palatine bones of the experimental group. The dogs of the experimental group were executed after 3,7,14,28,56 days of suture expansion. The change of suture tissue was examined by TEM. Results The cleft of the experiment group were closed at the ninth day of expansion. At the beginning, tissue rupture, exudation, death of fibroblasts, disruption of collagen and tear vessels were seen at the early stage of suture expansion. Then highly active functional manifestations were seen in both osteocytic and fibrocytic populations. At last, normal structure restored. Conclusions Cell types and functional condition could be distinguished clearly by TEM. It suggests that the suture expansion should be the process of tissue repair and regeneration. The suture cells response, especially, the osteogenic response were the major factor of increasing suture width.