CAJ | 학술논문

以药用野生稻(Oryza officinalis)的转育后代B5(高抗褐飞虱(Nilaparvata lugens Stl))与感虫品种明恢63 (Oryza sativa L.)为亲本,构建了一个重组自交系群体.通过抗褐飞虱鉴定,筛选出极端抗虫株系和极端感虫株系,运用分群分析法(bulked segregant analysis,BSA)分别建成了极端抗虫集团(resistant bulk)和极端感虫集团(susceptible bulk)的蛋白质池.利用双向电泳技术,分别分析了极端抗虫集团和极端感虫集团受虫害与未受虫害的秧苗蛋白质的变化.结果发现,虫害48 h后,感虫集团的一个分子量为40 kD的蛋白质P40 (pI=6.3)的表达明显减弱甚至消失,而在抗虫集团中,P40的表达未受影响.与褐飞虱为害后抗虫株系和感虫株系不同的生理反应相联系,推测P40与水稻受褐飞虱虫害后引起的应答反应相关.
이약용야생도(Oryza officinalis)적전육후대B5(고항갈비슬(Nilaparvata lugens Stl))여감충품충명회63 (Oryza sativa L.)위친본,구건료일개중조자교계군체.통과항갈비슬감정,사선출겁단항충주계화겁단감충주계,운용분군분석법(bulked segregant analysis,BSA)분별건성료겁단항충집단(resistant bulk)화겁단감충집단(susceptible bulk)적단백질지.이용쌍향전영기술,분별분석료겁단항충집단화겁단감충집단수충해여미수충해적앙묘단백질적변화.결과발현,충해48 h후,감충집단적일개분자량위40 kD적단백질P40 (pI=6.3)적표체명현감약심지소실,이재항충집단중,P40적표체미수영향.여갈비슬위해후항충주계화감충주계불동적생리반응상련계,추측P40여수도수갈비슬충해후인기적응답반응상관.
A recombinant inbred population (RI) was constructed from a cross between B5, an introgression line from the wild rice Oryza officinalis Wall. ex Watt, and susceptible cultivar Minghui 63 (O. sativa L.). The brown planthopper (BPH) resistances of RI lines were evaluated. Based on bulked segregant analysis (BSA), two protein bulks were made by extracting proteins from equally mixed seedlings of extremely resistant and susceptible plants selected from the RI population, respectively. Two-dimensional electrophoresis was used to detect the changes of polypeptide pattern. Results showed that a protein P40 (pI 6.3, Mw 40 kD) was significantly reduced or vanished after BPH infestation for 48 h in the susceptible bulk, while it remained uninfluenced in the resistant bulk. In connection with the physiological changes of the resistant and susceptible lines subjected to BPH sucking, we suppose that the protein P40 is related to the interaction responses of rice plants to BPH infestation.