北京大学学报(医学版)
北京大學學報(醫學版)
북경대학학보(의학판)
JOURNAL OF PEAKING UNIVERSITY(HEALTH SCIENCES)
2001年
2期
113-117
,共5页
表皮/代谢%Cx43%增殖细胞核抗原%细胞分裂期
錶皮/代謝%Cx43%增殖細胞覈抗原%細胞分裂期
표피/대사%Cx43%증식세포핵항원%세포분렬기
目的：研究大鼠前肢表皮角化细胞Cx43的表达和细胞增殖活性的拓扑学分布特点及二者间的关系。方法：常规石蜡切片，利用Cx43和增殖细胞核抗原（proliferating cell nuclear antigen，PCNA）免疫组化法，分析单位面积表皮细胞中Cx43表达的长度（LA）和PCNA阳性细胞核的百分率。结果：在水平方向上5 mm范围内，表皮细胞Cx43的表达随部位而异，且在距离始端大约3.0～4.5 mm处Cx43表达丰富。同时，在水平方向上10 mm范围内，角化细胞的增殖活性随部位而异，且增殖活性类似的角化细胞各自聚集成群，其直径大小约为10－3 m数量级；并在距离始端约3.0～4.0 mm处是角化细胞增殖活性低的位置。结论：大鼠表皮角化细胞间Cx43表达丰富处同时也是角化细胞增殖活性低的位置。
目的：研究大鼠前肢錶皮角化細胞Cx43的錶達和細胞增殖活性的拓撲學分佈特點及二者間的關繫。方法：常規石蠟切片，利用Cx43和增殖細胞覈抗原（proliferating cell nuclear antigen，PCNA）免疫組化法，分析單位麵積錶皮細胞中Cx43錶達的長度（LA）和PCNA暘性細胞覈的百分率。結果：在水平方嚮上5 mm範圍內，錶皮細胞Cx43的錶達隨部位而異，且在距離始耑大約3.0～4.5 mm處Cx43錶達豐富。同時，在水平方嚮上10 mm範圍內，角化細胞的增殖活性隨部位而異，且增殖活性類似的角化細胞各自聚集成群，其直徑大小約為10－3 m數量級；併在距離始耑約3.0～4.0 mm處是角化細胞增殖活性低的位置。結論：大鼠錶皮角化細胞間Cx43錶達豐富處同時也是角化細胞增殖活性低的位置。
목적：연구대서전지표피각화세포Cx43적표체화세포증식활성적탁복학분포특점급이자간적관계。방법：상규석사절편，이용Cx43화증식세포핵항원（proliferating cell nuclear antigen，PCNA）면역조화법，분석단위면적표피세포중Cx43표체적장도（LA）화PCNA양성세포핵적백분솔。결과：재수평방향상5 mm범위내，표피세포Cx43적표체수부위이이，차재거리시단대약3.0～4.5 mm처Cx43표체봉부。동시，재수평방향상10 mm범위내，각화세포적증식활성수부위이이，차증식활성유사적각화세포각자취집성군，기직경대소약위10－3 m수량급；병재거리시단약3.0～4.0 mm처시각화세포증식활성저적위치。결론：대서표피각화세포간Cx43표체봉부처동시야시각화세포증식활성저적위치。
Objective： To study topographic characters of the expression of Cx43 and the proliferation activity of keratinocytes on the upper legs of rats and to explore the relationship beween them. Methods： By rutine paraffin section , immunohistochemical techniques of Cx43 and PCNA were used to study the length of the expression of Cx43 in a unit area of epidermis(LA) and the percentage of proliferating cell nuclear antigen（PCNA）positive nucleus(%). Results： Within the range of 5 mm in the horizonal direction, the expression of Cx43 in epidermis varied according to the locations of keratinocytes and Cx43 was highly expressed which was about 3.0～4.5 mm from the start point. At the same time, within the range of 10 mm in the horizonal direction, the proliferation activity of keratinocytes was different from site to site and the kerationcytes with similar proliferation activity were clustered as groups, the scale of which was about 10－3 m; One of the sites, with low proliferation activity, was about 3.0～4.0 mm from the start point. Conclusion： This study suggested that the keratinocytes with low proliferation activity had high expression of Cx43.
