CAJ | 학술논문

采用国产C18反相柱，对以异硫氰酸苯酯(PITC)为柱前衍生化试剂的高效液相色谱测定氨基酸的方法进行了研究。在室温下，以pH=6.3的HAc-NaAc缓冲溶液及乙腈水溶液为流动相进行梯度洗脱，18种常见氨基酸在30min内获得了较好的分离，并且测定了珍珠角壳蛋白的氨基酸组成，结果表明：珍珠角壳蛋白主要由天冬氨酸、甘氨酸、丝氨酸、丙氨酸、亮氨酸等10种氨基酸组成，这些氨基酸约占蛋白总量的85%。
채용국산C18반상주，대이이류청산분지(PITC)위주전연생화시제적고효액상색보측정안기산적방법진행료연구。재실온하，이pH=6.3적HAc-NaAc완충용액급을정수용액위류동상진행제도세탈，18충상견안기산재30min내획득료교호적분리，병차측정료진주각각단백적안기산조성，결과표명：진주각각단백주요유천동안산、감안산、사안산、병안산、량안산등10충안기산조성，저사안기산약점단백총량적85%。
By use of octadecyl (C18) column made in China, a RP-HPLC method was studied using phenylisothiocyanate (PITC) as the pre-column derivatizing reagent for the determination of amino acids. Under room temperature, pH6.3 HAc-NaAc buffers, acetonitrile-water solutions as the mobile phase and the use of the gradient elution mode, fine resolution of phenylthiocarbamyl derivatives of 18 kinds of common amino acids was achieved after about 30 minutes and the content of amino acids (except Trp) in the hydrolysate of protein of pearl was determined. The results showed that the proteins in pearls mainly consist of 10 kinds of amino acids such as Asp, Glu, Ser, Gly, Ala, Leu and so on which account for about 85 percent of total amount of protein.