CAJ | 학술논문

目的从青岛海星Asterias rollestoni分离并确定结构新颖的活性化合物,并阐明海星中的次生代谢产物的多样性.方法应用正相和反相色谱包括半制备HPLC方法分离和纯化化合物;应用IR,UV,MS,lD和2D NMR,以及理化性质确定化合物的结构.结果从青岛海星Asterias rollestoni中获得17种化学成分,分别为N7-2'-deoxypseudoxanthosine(1),2'-O-methyl-in-osine(2),2'-deoxyinosine(3),2'-O-methylguanosine(4),inosine(5);thymine(6),uracil(7),thymidine(8),deoxyuridine(9),2'-O-methyluridine(10),(-)-(1S,3S)-1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid(11),(-)-(1R,3S)-1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid(12),(-)-(3S)-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid(13),L-tryptophan(14),L-phenylalanine(15),3-carboxy indole(16)和p-hydroxybenzoic acid(17).结论其中1为新化合物.它们的化学结构经1D,2D NMR,以及其它波谱分析,结合与已知化合物的波谱和理化数据对照得以确定.化合物8,9,10为首次从天然资源中获得,化合物14和15系首次从该海星中获得.
목적종청도해성Asterias rollestoni분리병학정결구신영적활성화합물,병천명해성중적차생대사산물적다양성.방법응용정상화반상색보포괄반제비HPLC방법분리화순화화합물;응용IR,UV,MS,lD화2D NMR,이급이화성질학정화합물적결구.결과종청도해성Asterias rollestoni중획득17충화학성분,분별위N7-2'-deoxypseudoxanthosine(1),2'-O-methyl-in-osine(2),2'-deoxyinosine(3),2'-O-methylguanosine(4),inosine(5);thymine(6),uracil(7),thymidine(8),deoxyuridine(9),2'-O-methyluridine(10),(-)-(1S,3S)-1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid(11),(-)-(1R,3S)-1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid(12),(-)-(3S)-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid(13),L-tryptophan(14),L-phenylalanine(15),3-carboxy indole(16)화p-hydroxybenzoic acid(17).결론기중1위신화합물.타문적화학결구경1D,2D NMR,이급기타파보분석,결합여이지화합물적파보화이화수거대조득이학정.화합물8,9,10위수차종천연자원중획득,화합물14화15계수차종해해성중획득.
Aim To investigate novel bioactive and structural metabolites from marine organisms. Methods Column chromatography in association with semi-preparative HPLC were used for the isolation of compounds. 1D and 2D NMR, IR,UV, and MS were employed for structure elucidation. Results From the butanol fraction of the 95 % EtOH extract of the starfish Asterias rollestoni, a new compound N7-2'-deoxypseudoxanthosine (1), along with sixteen known compounds, 2'-O-methyl-inosine (2), 2'-deoxyinesine (3), 2'-O-methylguanosine (4), inosine (5); thymine (6), uracil (7), thymidine (8), deoxyuridine (9), 2'-O-methyluridine (10), (-)-( 1S, 3S)-l-methyl-1, 2, 3, 4-tetrahydro-β-carboline-3-carboxyl-ic acid (11), ( - )-(1R, 3S)-l-methyl-1, 2, 3, 4-tetrahydro-β-cacboline-3-carboxylic acid (12), ( - )-(3S)- 1, 2, 3,4-tetrahydro-β-carboline-3-carboxylic acid (13), L-tryptophan (14), L-phenylalanine (15), 3-carboxy indole (16), and p-hydroxybenzoic acid (17), have been isolated. Conclusion Compound 1 is a new natural product, and compounds 8, 9 and 10 are isolated from natural sources for the first time, and the known compounds except 14 and 15 are first reported from starfish Asterias rollestoni.