CAJ | 학술논문

背景:心肌细胞在缺血再灌注损伤的过程中会加速细胞的凋亡,针刺手厥阴经穴位可抑制再灌注损伤的进程,减少凋亡的心肌细胞,从而呈现出对心脏的良好保护作用.目的:观察针刺手厥阴经穴对心肌缺血再灌注损伤过程中心肌细胞凋亡的影响.设计:以实验动物为研究对象的随机对照实验.单位:一所中医高等院校的针灸推拿系.材料:实验于2001-04/12在山东中医药大学中心实验室完成.选用Wistar大鼠40只,随机分为5组:空白组、模型组、针刺"内关"治疗组、针刺"郄门"治疗组、针刺"支沟"对照组.方法:电针大鼠心包经穴位20 min后,结扎左冠状动脉前降支40 min,心电图监测,再电针穴位20 min,松扎,恢复灌流60 min,摘取心脏,制成细胞悬液,流式细胞仪检测.主要观测指标:针刺对心肌细胞凋亡率变化的影响.结果:模型组出现典型的凋亡峰,凋亡率明显升高,针刺心包经穴组与模型组比较差异均有非常显著意义(P<0.01),其中针刺"内关"治疗组、针刺"郄门"治疗组、针刺"支沟"对照组及模型组的凋亡率分别为(4.29±0.76)%,(5.17±0.88)%,(13.96±2.77)%,(16.01±1.04)%.结论:针刺手厥阴经穴能有效地抑制心肌细胞的凋亡,从而减轻由于凋亡细胞导致的心肌损伤.
배경:심기세포재결혈재관주손상적과정중회가속세포적조망,침자수궐음경혈위가억제재관주손상적진정,감소조망적심기세포,종이정현출대심장적량호보호작용.목적:관찰침자수궐음경혈대심기결혈재관주손상과정중심기세포조망적영향.설계:이실험동물위연구대상적수궤대조실험.단위:일소중의고등원교적침구추나계.재료:실험우2001-04/12재산동중의약대학중심실험실완성.선용Wistar대서40지,수궤분위5조:공백조、모형조、침자"내관"치료조、침자"극문"치료조、침자"지구"대조조.방법:전침대서심포경혈위20 min후,결찰좌관상동맥전강지40 min,심전도감측,재전침혈위20 min,송찰,회복관류60 min,적취심장,제성세포현액,류식세포의검측.주요관측지표:침자대심기세포조망솔변화적영향.결과:모형조출현전형적조망봉,조망솔명현승고,침자심포경혈조여모형조비교차이균유비상현저의의(P<0.01),기중침자"내관"치료조、침자"극문"치료조、침자"지구"대조조급모형조적조망솔분별위(4.29±0.76)%,(5.17±0.88)%,(13.96±2.77)%,(16.01±1.04)%.결론:침자수궐음경혈능유효지억제심기세포적조망,종이감경유우조망세포도치적심기손상.
BACKGROUND: Apoptosis of cardiomyocytes is speeded up during ischemic reperfused injury. Acupuncture on the points of hand- Jueyin meridian can inhibit the progression of reperfused injury and reduce apoptosis of cardiomyocytes so as to provide the protection on the heart.OBJECTIVE: To observe the effects of acupuncture on the points of hand-Jueyin meridian on apoptosis of cardiomyocytes during ischemic reperfused injury.DESIGN: Randomized controlled experiment based on the experimental animals.SETTING: Department of acupuncture and massage in a university of traditional Chinese medicine.MATERIALS: The experiment was performed in Central Laboratory of Shandong University of Traditional Chinese Medicine from April to December 2001. Forty Wistar male rats were employed, and were randomized into 5 groups, named as blank group, model group, "Neiguan(PC 6)" group,"Ximen(PC 4)" group and "Zhigou(TE 6)" group.METHODS: After electric acupuncture on the points of pericardium meridian for 20 minutes, the ligature was done on the anterior-descending branch of the left coronary artery for 40 minutes. Electrocardiogram (ECG) monitoring was followed. The ligature was released after electric acupuncture on the points for another 20 minutes and perfusion was restored for 60 minutes. Afterward, the heart was extracted, the cellular suspension was prepared and the determination with flow-type cell apparatus was applied.MAIN OUTCOME MEASURES: Effects of acupuncture on the changes in apoptosis rate of cardiomyocytes.RESULTS: In the model group, the typical apoptosis peak presented, the apoptosis rate was remarkably increased. The comparison between the points of pericardium meridian groups and the model group indicated very significant difference(P < 0.01) .The apoptosis rates in "Neiguan(PC 6)" group, "Ximen(PC 4)" group and "Zhigou(TE 6)" group as well as the model groupwere(4.29±0.76)%, (5. 17±0.88)%, (13.96 ±2.77)% and (16.01 ± 1.04)% respectively.CONCLUSION: Acupuncture on the points of hand-Jueyin meridian inhibits myocardial apoptosis effectively so as to reduce myocardial injury caused by cell apoptosis.