CAJ | 학술논문

背景:目前国内外关于放射性脑损伤动物模型的研究均处于探索中,尚未形成成熟的模型制作方法.目的:建立急性放射性脑损伤的鼠模型,为进一步研究放射性脑损伤正确有效的预防措施提供实验依据.设计:以实验动物为研究对象,随机对照观察研究.单位:一所大学医院动物实验室.材料:实验于2001-06/2002-08在中山大学附属第二医院实验室完成.SD大鼠60只,体质量(300±30)g,雌雄各半,空白对照组20只,实验组40只.方法:SD大鼠头部接受60Coγ射线照射,7 Gy/次,1次/d,连续照射6 d,总剂量42 Gy.照射结束后每日观察大鼠摄食、饮水量及次数,自主活动情况,有无出现神经系统症状与体征,每周检查并记录大鼠头部照射区毛发与皮肤情况、体质量变化,照射结束后第3,7,14,30天断头取脑,行病理组织学检查. 主要观察指标:①一般情况观察.②照射后脑组织病理改变.结果:照射第3天起即出现每日摄食、饮水量减少;照射第1,2天,自主活动较对照组增多,第3天起活动渐减少;无异常神经系统体征;随观察时间延长体质量增长较对照组慢,但差异无显著性意义;所有大鼠均于照射后约2周时出现照射野轻度脱毛;照射后出现脑组织神经元变性坏死.结论:该模型制作方法切实、可靠,较好地模拟放射性脑损伤的过程,可用于预防或减轻放射治疗对脑组织损伤的实验研究.
배경:목전국내외관우방사성뇌손상동물모형적연구균처우탐색중,상미형성성숙적모형제작방법.목적:건립급성방사성뇌손상적서모형,위진일보연구방사성뇌손상정학유효적예방조시제공실험의거.설계:이실험동물위연구대상,수궤대조관찰연구.단위:일소대학의원동물실험실.재료:실험우2001-06/2002-08재중산대학부속제이의원실험실완성.SD대서60지,체질량(300±30)g,자웅각반,공백대조조20지,실험조40지.방법:SD대서두부접수60Coγ사선조사,7 Gy/차,1차/d,련속조사6 d,총제량42 Gy.조사결속후매일관찰대서섭식、음수량급차수,자주활동정황,유무출현신경계통증상여체정,매주검사병기록대서두부조사구모발여피부정황、체질량변화,조사결속후제3,7,14,30천단두취뇌,행병리조직학검사. 주요관찰지표:①일반정황관찰.②조사후뇌조직병리개변.결과:조사제3천기즉출현매일섭식、음수량감소;조사제1,2천,자주활동교대조조증다,제3천기활동점감소;무이상신경계통체정;수관찰시간연장체질량증장교대조조만,단차이무현저성의의;소유대서균우조사후약2주시출현조사야경도탈모;조사후출현뇌조직신경원변성배사.결론:해모형제작방법절실、가고,교호지모의방사성뇌손상적과정,가용우예방혹감경방사치료대뇌조직손상적실험연구.
BACKGROUND: At present, the researches on murine model of acute radiation encephalopathy are still in investigation, and mature model making method is not clear.OBJECTIVE: To establish a murine model of acute radiation encephalopathy in order to provide a good foundation for further researches of radiation encephalopathy(REP) mechanism and therapy.DESIGN: Randomized controlled study based on the experimental animals.SETTING: Animal laboratory in a university hospital. The Second Affiliated Hospital of Sun Yat-sen University.MATERIALS: The experiment was completed in the Laboratory of the Second Affiliated Hospital of Sun Yat-sen University from June 2001 to August 2002. Totally 30 female and 30 male SD rats, weighted(300 ± 30) g, were selected from the Animal Experiment Center of Sun Yat-sen University, and randomly divided into blank control group with 20 rats and experimental group with 40 rats.METHODS: Fourty rats' brain received 60Co γ-ray irradiation with the dosage of 7 Gy/time per day for 6 consecutive days with the total dosage of 42 Gy. The amount of ingestion and drinking, general activities, central nervous system(CNS) symptoms and signs were recorded every day. The hairs and skin of irradiated field and weight were checked and recorded weekly. On the 3rd, 7th, 14th, 30th days after radiation, the brain tissue was collected and the histopathologic changes were examined.Histopathologic changes after radiation.RESULTS: Since the third day, the ingestion and drinking amount of irradiated rats were decreased. The general activities were increased for the first two days, but decreased without abnormal nervous signs on the 3rd day. The rats in experimental group had a slower weight gain than those of control group, and the difference between them was of no statistical significance. All rats had slight alopecia and neuronal necrosis 2 weeks after irradiation.CONCLUSION: The irradiation method is reliable, practical and good for modeling REP process, which can be used in preventing or reducing the harm effect of radiation therapy on brain tissue.