CAJ | 학술논문

目的观察未成年Balb/c小鼠小肠Cajal间质细胞(ICC)的形态及分布,并测定其慢波活动情况,以进一步认识ICC的发育.方法首先,通过免疫组织化学方法观察10天大小Balb/c小鼠小肠ICC的分布及形态特征,采用电镜观察ICC的超微结构;再分别测定10d大小Balb/c小鼠及成年Balb/c小鼠小肠在体慢波活动情况.结果 ICC主要分布于小肠环肌层与纵肌层间区(称之为ICC-MY),以及环形肌的内薄层与外厚层间(称之为ICC-DMP).ICC-MY呈现为连续性分布特点.电镜结果显示ICC有2个以上长突起,胞浆内含有大量的线粒体,而中间丝很少见.成年小鼠小肠慢波活动表现为近似正弦波样曲线,频率为(17.78±1.52)次/min,振幅为(0.16±0.02)mV,10 d大小Balb/c小鼠小肠慢波活动类似于成年小鼠,但其频率为(14.00±0.98)次/分,振幅为(0.09±0.02)mV,均明显低于成年小鼠(P＜0.01).结论 Balb/c小鼠小肠主要分布着ICC-MY及ICC-DMP两类ICC,为双极或多极细胞,ICC-MY相互连接成网络状;未完全发育好的ICC表现出不成熟的慢波活动.
목적 관찰미성년Balb/c소서소장Cajal간질세포(ICC)적형태급분포,병측정기만파활동정황,이진일보인식ICC적발육.방법 수선,통과면역조직화학방법관찰10천대소Balb/c소서소장ICC적분포급형태특정,채용전경관찰ICC적초미결구;재분별측정10d대소Balb/c소서급성년Balb/c소서소장재체만파활동정황.결과 ICC주요분포우소장배기층여종기층간구(칭지위ICC-MY),이급배형기적내박층여외후층간(칭지위ICC-DMP).ICC-MY정현위련속성분포특점.전경결과현시ICC유2개이상장돌기,포장내함유대량적선립체,이중간사흔소견.성년소서소장만파활동표현위근사정현파양곡선,빈솔위(17.78±1.52)차/min,진폭위(0.16±0.02)mV,10 d대소Balb/c소서소장만파활동유사우성년소서,단기빈솔위(14.00±0.98)차/분,진폭위(0.09±0.02)mV,균명현저우성년소서(P＜0.01).결론 Balb/c소서소장주요분포착ICC-MY급ICC-DMP량류ICC,위쌍겁혹다겁세포,ICC-MY상호련접성망락상;미완전발육호적ICC표현출불성숙적만파활동.
[Objective] To observe the distribution and morphology of interstitial cells of Cajal (ICC) in young Balb/c murine small intestine and determine the activities of slow waves, and to recognize the development of ICC more clearly. [Methods] Firstly, the distribution and configuration of ICC were observed with immunohistochemical method and the ultrastructure of ICC was observed by an electron microscope in the small intestine of 10-days postpartum Balb/c murine. Secondly, the slow waves of small intestine in vivo were measured in 10-days postpartum Balb/c murine and in adult Balb/c murine. [Results] ICC were located mainly between the circular and longitudinal muscle layers (called ICC-MY) and between the internal thin layer and the thick outer layer of the circular muscle(called ICC-DMP) in the small intestine. The distribution of ICC-MY was continuous. Electron microscope showed that ICC had two or more very long main processes and contained abundant mitochondria, while intermediate filaments were rarely seen. The slow waves of small intestine were proximal cosinal waves in the adult Balb/c murine and the frequency was (17.78±1.52) cycles/min and the amplitude was (0.16±0.02) mV. The slow waves in the 10-days postpartum Balb/c murine resembled those in adult murine and the frequency was (14.00±0.98) cycles/min and the amplitude was (0.09±0.02) mV (P＜0.01 vs the adult murine). [Conclusion] Two types of ICC including ICC-MY and ICC-DMP are observed in the young Balb/c murine small intestine which are bipolar or multipolar cells.ICC-MY interconnects with a network. ICC that are undeveloped fully has immature slow waves.