CAJ | 학술논문

目的对丙型肝炎病毒（HCV）包膜区E2基因进行克隆与表达，并将纯化的E2蛋白用于对HCV感染者血清中E2抗体的检测。方法首先从HCV感染者血清中经随机引物反转录和聚合酶链反应（PCR）获得E2区基因片段，然后克隆入原核表达系统pQE-30中进行蛋白表达、纯化，并检测表达蛋白的抗原性。结果获得一段具有正确读码框架的E2区基因，全长984bp，表达蛋白分子量为38kD。经与HCV感染者血清进行ELISA检测，证明其具有一定的抗原性。结论本实验为研究HCV外膜蛋白的基本特点、血清学诊断意义和HCV疫苗设计提供了基础资料。
목적대병형간염병독（HCV）포막구E2기인진행극륭여표체，병장순화적E2단백용우대HCV감염자혈청중E2항체적검측。방법수선종HCV감염자혈청중경수궤인물반전록화취합매련반응（PCR）획득E2구기인편단，연후극륭입원핵표체계통pQE-30중진행단백표체、순화，병검측표체단백적항원성。결과획득일단구유정학독마광가적E2구기인，전장984bp，표체단백분자량위38kD。경여HCV감염자혈청진행ELISA검측，증명기구유일정적항원성。결론본실험위연구HCV외막단백적기본특점、혈청학진단의의화HCV역묘설계제공료기출자료。
Objective　To clone and express the E2 gene in envelope region of hepatitis C virus and detect the anti-E2 in sera of patients with HCV infection,using the purified E2 Protein.Methods　The gene fragment of E2 region was obtained from the sera of patients with HCV infection by random primer reverse transcription and polymerase chain reaction(PCR),and then cloned into procaryotic expression system to express and Purify the protein and detect its antigenicity.Results　A segment of E2 region gene with correct code-reading frame was obtained,its full length was 984 bp and molecular weight of expressed Protein was 38kD.The detection of the protein performed by ELISA with sera from the patients with HCV infection,testified that it had appropriate antigenicity.Conclusion　This experiment can provide the fundamental data for researching on the basic characteristics of HCV envelope protein,nuderstanding the significance of serological diagnosis and designing the anti-HCV vassine.