安徽医药
安徽醫藥
안휘의약
ANHUI MEDICAL AND PHARMACEUTICAL JOURNAL
2009年
7期
751-752
,共2页
依托泊苷植入剂%含量%有关物质%高效液相色谱法
依託泊苷植入劑%含量%有關物質%高效液相色譜法
의탁박감식입제%함량%유관물질%고효액상색보법
etoposide implants%content%related substances%HPLC
目的 建立一种常规高效液相色谱法测定依托泊苷植入剂的有关物质及含量.方法 采用C18柱,乙腈-醋酸盐缓冲液(pH4.0)(2∶ 5)(取醋酸钠5.44 g,加水溶解并稀释至2000 ml,用冰醋酸调节pH值至4.0)为流动相,检测波长:230 nm.降解产物制备方法 为将溶液置80℃水浴中破坏30 min.结果 该法能很好地分离依托泊苷及有关物质,检测限为0.05 μg.结论 本方法 同时检测依托泊苷植入剂的有关物质及含量,方法 准确、方便、实用性强.
目的 建立一種常規高效液相色譜法測定依託泊苷植入劑的有關物質及含量.方法 採用C18柱,乙腈-醋痠鹽緩遲液(pH4.0)(2∶ 5)(取醋痠鈉5.44 g,加水溶解併稀釋至2000 ml,用冰醋痠調節pH值至4.0)為流動相,檢測波長:230 nm.降解產物製備方法 為將溶液置80℃水浴中破壞30 min.結果 該法能很好地分離依託泊苷及有關物質,檢測限為0.05 μg.結論 本方法 同時檢測依託泊苷植入劑的有關物質及含量,方法 準確、方便、實用性彊.
목적 건립일충상규고효액상색보법측정의탁박감식입제적유관물질급함량.방법 채용C18주,을정-작산염완충액(pH4.0)(2∶ 5)(취작산납5.44 g,가수용해병희석지2000 ml,용빙작산조절pH치지4.0)위류동상,검측파장:230 nm.강해산물제비방법 위장용액치80℃수욕중파배30 min.결과 해법능흔호지분리의탁박감급유관물질,검측한위0.05 μg.결론 본방법 동시검측의탁박감식입제적유관물질급함량,방법 준학、방편、실용성강.
Aim To establish an HPLC method for the determination of etoposide implants and related substances.Methods C18 column was adopted.The mobile phase was composed of acetonitrile-acetate buffer solution (2∶ 5),with 5.44 g of sodium acetate dissolved in 2000 ml of water,adjusted with glacial acetic acid to a pH of 4.0.The detection was carried out with a UV detector at 230nm.The preparative method of the degradation product was to put the solution in the water-bath with a temperature of 80℃ for 30 min.Result The method could separate etoposide from the relative substances.The detection limit was 0.05 μg.Conclusion The method is good and accurate.
