海洋通报
海洋通報
해양통보
MARINE SCIENCE BULLETIN
2009年
6期
90-94
,共5页
宗虎民%马德毅%王菊英%户江涛
宗虎民%馬德毅%王菊英%戶江濤
종호민%마덕의%왕국영%호강도
氟苯尼考%沉积物%纤维素酶%过氧化氢酶%多酚氧化酶
氟苯尼攷%沉積物%纖維素酶%過氧化氫酶%多酚氧化酶
불분니고%침적물%섬유소매%과양화경매%다분양화매
florfenicol%sediment%cellulase%catalase%polyphenol oxidase
通过室内模拟实验研究了氟苯尼考对海洋沉积物中纤维素酶、过氧化氢酶和多酚氧化酶活性的影响.结果表明,在整个培养期间,低浓度(10 mg/kg)的氟苯尼考对纤维素酶和过氧化氢酶活性没有明显影响,而较高浓度(100 mg/kg和500 mg/kg)则对过氧化氢酶活性具有明显的抑制作用,且随着氟苯尼考浓度的增加抑制作用加大.氟苯尼考在初始阶段对沉积物中多酚氧化酶的活性有显著的抑制作用;随着培养时间的延长,低浓度(10 mg/kg)的氟苯尼考对酶活性的影响变得不明显,而较高浓度(100 mg/kg和500 mg/kg)的氟苯尼考在整个培养期间对多酚氧化酶的活性都具有显著的抑制作用.
通過室內模擬實驗研究瞭氟苯尼攷對海洋沉積物中纖維素酶、過氧化氫酶和多酚氧化酶活性的影響.結果錶明,在整箇培養期間,低濃度(10 mg/kg)的氟苯尼攷對纖維素酶和過氧化氫酶活性沒有明顯影響,而較高濃度(100 mg/kg和500 mg/kg)則對過氧化氫酶活性具有明顯的抑製作用,且隨著氟苯尼攷濃度的增加抑製作用加大.氟苯尼攷在初始階段對沉積物中多酚氧化酶的活性有顯著的抑製作用;隨著培養時間的延長,低濃度(10 mg/kg)的氟苯尼攷對酶活性的影響變得不明顯,而較高濃度(100 mg/kg和500 mg/kg)的氟苯尼攷在整箇培養期間對多酚氧化酶的活性都具有顯著的抑製作用.
통과실내모의실험연구료불분니고대해양침적물중섬유소매、과양화경매화다분양화매활성적영향.결과표명,재정개배양기간,저농도(10 mg/kg)적불분니고대섬유소매화과양화경매활성몰유명현영향,이교고농도(100 mg/kg화500 mg/kg)칙대과양화경매활성구유명현적억제작용,차수착불분니고농도적증가억제작용가대.불분니고재초시계단대침적물중다분양화매적활성유현저적억제작용;수착배양시간적연장,저농도(10 mg/kg)적불분니고대매활성적영향변득불명현,이교고농도(100 mg/kg화500 mg/kg)적불분니고재정개배양기간대다분양화매적활성도구유현저적억제작용.
Effects of florfenicol on the activities of cellulase,catalase,polyphenol oxidase in marine sediment were studied in laboratory by simulation experiment.At lower concentration(10 mg/kg),florfenicol did not affect the activities of cellulase and catalase significantly,while it inhibited the enzymatic activities at higher concentrations(100 mg/kg and 500 mg/kg) during the whole incubation,and inhibition became more severe with increasing of florfenicol concentrations.Florfenicol at 10 mg/kg inhibited the activity of polyphenol oxidase in marine sediment at the early stage of incubation,but it became no significant difference with the prolonging of the incubation time,and inhibition occurred with the treatment of florfenicol at higher concentrations(100 mg/kg and 500 mg/kg).