中国骨质疏松杂志
中國骨質疏鬆雜誌
중국골질소송잡지
CHINESE JOURNAL OF OSTEOPOROSIS
2001年
1期
9-11
,共3页
金慰芳%朱文菁%王洪复%骆宏丰%胡昌奇
金慰芳%硃文菁%王洪複%駱宏豐%鬍昌奇
금위방%주문정%왕홍복%락굉봉%호창기
补肾中药%细胞培养%成骨细胞
補腎中藥%細胞培養%成骨細胞
보신중약%세포배양%성골세포
目的 为了解补肾中药HU-ECS对体外培养成骨细胞的作用。 方法 应用MTT法、对硝基苯磷酸盐法及茜素红染色方法观察HU-ECS对体 外培养成骨细胞的增殖、ALP表达及矿化结节形成的影响。 结果 HU-ECS具有刺激成骨细胞增殖作用(10-6 g/ml组,P<0 .01~0.001),提高ALP活性及矿化结节形成的数量。 结论 HU-ECS具有刺激体外培养成骨细胞增殖及分化成熟的功能。
目的 為瞭解補腎中藥HU-ECS對體外培養成骨細胞的作用。 方法 應用MTT法、對硝基苯燐痠鹽法及茜素紅染色方法觀察HU-ECS對體 外培養成骨細胞的增殖、ALP錶達及礦化結節形成的影響。 結果 HU-ECS具有刺激成骨細胞增殖作用(10-6 g/ml組,P<0 .01~0.001),提高ALP活性及礦化結節形成的數量。 結論 HU-ECS具有刺激體外培養成骨細胞增殖及分化成熟的功能。
목적 위료해보신중약HU-ECS대체외배양성골세포적작용。 방법 응용MTT법、대초기분린산염법급천소홍염색방법관찰HU-ECS대체 외배양성골세포적증식、ALP표체급광화결절형성적영향。 결과 HU-ECS구유자격성골세포증식작용(10-6 g/ml조,P<0 .01~0.001),제고ALP활성급광화결절형성적수량。 결론 HU-ECS구유자격체외배양성골세포증식급분화성숙적공능。
Objective To study the effects of HU-E CS extracted from traditional Chinese medicine on cultured osteoblasts in vit ro. Methods MTT,PNPP and ARS were used to observe t he proliferation,activity of ALP and the number of mineral nodes of osteoblasts cultured in vitro. Results It was found that HU-ECS had the effects on stimulati ng the proliferation,ALP activity and the number of mineral nodes of cultured os teoblasts In 10-6 g/ml concentration,the effect was stable and notable. Conclusion HU-ECS has the effects on stimulating the prolifera tion,differentiation and maturation of cultured osteoblasts in vitro.