中国小儿急救医学
中國小兒急救醫學
중국소인급구의학
CHINESE PEDIATRIC EMERGENCY MEDICINE
2008年
2期
144-147
,共4页
王红%宋朝敏%吴斌%黄妙辉
王紅%宋朝敏%吳斌%黃妙輝
왕홍%송조민%오빈%황묘휘
坏死性小肠结肠炎%新生鼠%动物模型%血小板活化因子%肿瘤坏死因子
壞死性小腸結腸炎%新生鼠%動物模型%血小闆活化因子%腫瘤壞死因子
배사성소장결장염%신생서%동물모형%혈소판활화인자%종류배사인자
Necrotizing enterocolitis%Neonatal rat%Animal model%Platelet-activating factor%Tumor necrosis factor-α
目的 研究新生鼠肠损伤时肠组织中血小板活化因子(PAF)、肿瘤坏死因子(TNF)-α含量变化及肠组织损伤程度与肠组织中PAF、TNF-α表达水平的关系,探讨将PAF、TNF-α表达水平作为肠损伤的检测指标的可能性.方法 按析因设计,32只48 h新生SD大鼠随机分成4组,每组各8只.A组给予鼠乳代用品人工喂养,每天2次连续3 d给予100%氮气缺氧90 s,4℃冷刺激10 min;B组用鼠乳代用品人工喂养3 d,未受缺氧和冷刺激;C组每天2次连续3 d给予100%氮气缺氧90 s,4℃冷刺激10min、鼠母乳喂养;D组为正常对照组.在实验结束后24 h空腹断头处死大鼠,留取十二指肠下端至直肠上端肠道组织进行肠组织损伤评分和肠组织中PAF、TNF-α含量检测.组织学评分≥2确定为坏死性小肠结肠炎(NEC);肠组织中PAF、TNF-α含量采用ELISA双抗体夹心法检测.应用Kruskal-Wallis H检验等方法进行统计学分析,α=0.05为显著性检验标准.结果 经Kruskal-Wallis H检验,A、B、C、D各组肠组织PAF、TNF-α含量不同,差异有显著性.采用非参数Spearman等级相关分析结果 ,肠组织PAF含量与相应平均损伤程度均呈显著正相关关系(r=0.71,P＜0.05);肠组织TNF-α含量与相应平均损伤程度均呈显著正相关关系(r=0.81,P<0.05).结论 肠组织内源性聊小和TNF-a可能是NEC发生的关键介质,其本身又作为致病因子参与了NEC的发生和发展.肠损伤程度与肠组织中PAF、TNF-α含量呈正相关,肠组织中PAF和TNF-α含量与肠黏膜损伤评分一样能反映肠黏膜损伤程度.
目的 研究新生鼠腸損傷時腸組織中血小闆活化因子(PAF)、腫瘤壞死因子(TNF)-α含量變化及腸組織損傷程度與腸組織中PAF、TNF-α錶達水平的關繫,探討將PAF、TNF-α錶達水平作為腸損傷的檢測指標的可能性.方法 按析因設計,32隻48 h新生SD大鼠隨機分成4組,每組各8隻.A組給予鼠乳代用品人工餵養,每天2次連續3 d給予100%氮氣缺氧90 s,4℃冷刺激10 min;B組用鼠乳代用品人工餵養3 d,未受缺氧和冷刺激;C組每天2次連續3 d給予100%氮氣缺氧90 s,4℃冷刺激10min、鼠母乳餵養;D組為正常對照組.在實驗結束後24 h空腹斷頭處死大鼠,留取十二指腸下耑至直腸上耑腸道組織進行腸組織損傷評分和腸組織中PAF、TNF-α含量檢測.組織學評分≥2確定為壞死性小腸結腸炎(NEC);腸組織中PAF、TNF-α含量採用ELISA雙抗體夾心法檢測.應用Kruskal-Wallis H檢驗等方法進行統計學分析,α=0.05為顯著性檢驗標準.結果 經Kruskal-Wallis H檢驗,A、B、C、D各組腸組織PAF、TNF-α含量不同,差異有顯著性.採用非參數Spearman等級相關分析結果 ,腸組織PAF含量與相應平均損傷程度均呈顯著正相關關繫(r=0.71,P＜0.05);腸組織TNF-α含量與相應平均損傷程度均呈顯著正相關關繫(r=0.81,P<0.05).結論 腸組織內源性聊小和TNF-a可能是NEC髮生的關鍵介質,其本身又作為緻病因子參與瞭NEC的髮生和髮展.腸損傷程度與腸組織中PAF、TNF-α含量呈正相關,腸組織中PAF和TNF-α含量與腸黏膜損傷評分一樣能反映腸黏膜損傷程度.
목적 연구신생서장손상시장조직중혈소판활화인자(PAF)、종류배사인자(TNF)-α함량변화급장조직손상정도여장조직중PAF、TNF-α표체수평적관계,탐토장PAF、TNF-α표체수평작위장손상적검측지표적가능성.방법 안석인설계,32지48 h신생SD대서수궤분성4조,매조각8지.A조급여서유대용품인공위양,매천2차련속3 d급여100%담기결양90 s,4℃랭자격10 min;B조용서유대용품인공위양3 d,미수결양화랭자격;C조매천2차련속3 d급여100%담기결양90 s,4℃랭자격10min、서모유위양;D조위정상대조조.재실험결속후24 h공복단두처사대서,류취십이지장하단지직장상단장도조직진행장조직손상평분화장조직중PAF、TNF-α함량검측.조직학평분≥2학정위배사성소장결장염(NEC);장조직중PAF、TNF-α함량채용ELISA쌍항체협심법검측.응용Kruskal-Wallis H검험등방법진행통계학분석,α=0.05위현저성검험표준.결과 경Kruskal-Wallis H검험,A、B、C、D각조장조직PAF、TNF-α함량불동,차이유현저성.채용비삼수Spearman등급상관분석결과 ,장조직PAF함량여상응평균손상정도균정현저정상관관계(r=0.71,P＜0.05);장조직TNF-α함량여상응평균손상정도균정현저정상관관계(r=0.81,P<0.05).결론 장조직내원성료소화TNF-a가능시NEC발생적관건개질,기본신우작위치병인자삼여료NEC적발생화발전.장손상정도여장조직중PAF、TNF-α함량정정상관,장조직중PAF화TNF-α함량여장점막손상평분일양능반영장점막손상정도.
Objective To study the change of platelet-activating factor(PAF)and tumor necrosis fac-tor-α(TNF-α)density in neonatal rat model of necrotizing enterocolitis,and analyze the correlation between the PAF、TNF-α levd and the scoring of intestinal injury.The aim is to explore the function of PAF and TNF-α in NEC.Methods According to 2 x 2 factor analysis,32 neonate Sprague-Dewley rats(48 hours olds)were divided into 4 groups(A,B,C and D,8 rats each group).Group A was made into NEC models as follows:separate from mother rats and feeding with rat milk substitute,hypoxia(100%N2)for 90 second and 4℃cold exposure for 10 minutes,twice a day during 3 consecutive days;Group B was given formula feeding alone,not hypoxia and cold exposure.Group C was given rat milk feeding,the same hypoxia and cold exposure as group A,Group D was control group,given rat milk feeding,not hypoxia and cold exposure.On the 4th day all the subjects were sacrificed and the intestine around ileocecal junction were obtained to evaluated by stained with H&E for histological analysis and score.The mean scores more than 2 were considered NEC.The contents of PAF,TNF-α in homogenate of intestinal tissue were measured by ELISA(Kits bought from Shanghai Langka Company).Kruskal-Wallis H test,Spearman correlation analysis were used to analyze difference among various groups.α=0.05 was considered significant.Results The score of histopatholigical and the contents of PAF,TNF-α had significant difference among groups(P＜0.01).By spearman correlation analysis,the relations of the degree of intestinal injury and the contents of PAF,TNF-α in intestinal tissue were positive(rpAF=0.71,rTNF-α=0.81,P＜0.05).Conclusion Endogenous PAF and TNF-α in intestine tissue are probably the critical factors for the development of NEC.As risk factors,they are involed in the development of NEC.It indicated that the concentrations of PAF and TNF-α in intestine tissue had a positive correlation with the severity of NEC and can inflect the degree of intestine mucosa lesions.
