中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2010年
4期
264-267
,共4页
胡雄飞%李静%庄宛%李龙
鬍雄飛%李靜%莊宛%李龍
호웅비%리정%장완%리룡
氟虫腈%PCI2细胞%基因,Bcl-2
氟蟲腈%PCI2細胞%基因,Bcl-2
불충정%PCI2세포%기인,Bcl-2
Fipronil%PC12 cells%Gene,Dcl-2
目的 研究氟虫腈对神经细胞凋亡的影响并初步探讨其神经毒作用机制.方法 设3.13×10~(-6)、1.25×10~(-5)、5.00×10~(-5)mol/L 3个氟虫腈染毒组和1个对照组,用荧光显微镜检测各组PC12细胞凋亡荧光强度并用流式细胞仪对凋亡率进行测定,并对3个染毒组Bcl-2蛋白表达进行荧光强度检测.结果 细胞凋亡荧光强度检测发现,3.13×10~(-6)mol/L染毒组细胞凋亡率为17.00%±0.57%,5.00×10~(-5)mol/L染毒组细胞坏死率为13.95%±1.95%,均明显高于对照组(分别为9.80%±0.30%、3.10%±0.40%),差异均有统计学意义(P<0.05).对Bcl-2蛋白表达含量进行定性检测,结果显示,3.13×10~(-6)mol/L染毒组Bcl-2蛋白表达明显低于对照组,其他染毒组Bcl-2蛋白表达与对照组比较无明显差异.结论 氟虫腈在较低剂量时可能是通过影响Bcl-2蛋白表达来诱导PCI2细胞凋亡,而在较高剂量时可能通过其他非细胞凋亡途径直接致细胞坏死发生.
目的 研究氟蟲腈對神經細胞凋亡的影響併初步探討其神經毒作用機製.方法 設3.13×10~(-6)、1.25×10~(-5)、5.00×10~(-5)mol/L 3箇氟蟲腈染毒組和1箇對照組,用熒光顯微鏡檢測各組PC12細胞凋亡熒光彊度併用流式細胞儀對凋亡率進行測定,併對3箇染毒組Bcl-2蛋白錶達進行熒光彊度檢測.結果 細胞凋亡熒光彊度檢測髮現,3.13×10~(-6)mol/L染毒組細胞凋亡率為17.00%±0.57%,5.00×10~(-5)mol/L染毒組細胞壞死率為13.95%±1.95%,均明顯高于對照組(分彆為9.80%±0.30%、3.10%±0.40%),差異均有統計學意義(P<0.05).對Bcl-2蛋白錶達含量進行定性檢測,結果顯示,3.13×10~(-6)mol/L染毒組Bcl-2蛋白錶達明顯低于對照組,其他染毒組Bcl-2蛋白錶達與對照組比較無明顯差異.結論 氟蟲腈在較低劑量時可能是通過影響Bcl-2蛋白錶達來誘導PCI2細胞凋亡,而在較高劑量時可能通過其他非細胞凋亡途徑直接緻細胞壞死髮生.
목적 연구불충정대신경세포조망적영향병초보탐토기신경독작용궤제.방법 설3.13×10~(-6)、1.25×10~(-5)、5.00×10~(-5)mol/L 3개불충정염독조화1개대조조,용형광현미경검측각조PC12세포조망형광강도병용류식세포의대조망솔진행측정,병대3개염독조Bcl-2단백표체진행형광강도검측.결과 세포조망형광강도검측발현,3.13×10~(-6)mol/L염독조세포조망솔위17.00%±0.57%,5.00×10~(-5)mol/L염독조세포배사솔위13.95%±1.95%,균명현고우대조조(분별위9.80%±0.30%、3.10%±0.40%),차이균유통계학의의(P<0.05).대Bcl-2단백표체함량진행정성검측,결과현시,3.13×10~(-6)mol/L염독조Bcl-2단백표체명현저우대조조,기타염독조Bcl-2단백표체여대조조비교무명현차이.결론 불충정재교저제량시가능시통과영향Bcl-2단백표체래유도PCI2세포조망,이재교고제량시가능통과기타비세포조망도경직접치세포배사발생.
Objecfive To study the apoptosis and mechanism of Fipmnil on PC12 Cells.Methods The effect of fipmnil on the apoptosis and necrosis of PCI2 cells of 3.13×10~(-6), 1.25×10~(-5),5.00×10~(-5) mol/L three dose groups after 24 h treatment was detected by morphology and the apoptosis rate Was detected by flow cytometer(FCM)The expression of Bcl-2 protein in PC12 ceUs of 3.13×10~(-6),1.25×10~(-5)and 5.00×10~(-5)mol/L three dose groups after 24 h treatment Was measured by immunofluoreseence.Results The number of apop-totic cells of 3.13×10~(-6)mol/L group was more than the control group examined by fluorescence microscope,and the number of dead cells of 5.00×10~(-5)mol/L group was more than the control group.The apoptotic rates of Pc12 cells wag higher in the 3.13×10~(-6)mol/L group than the control group measured by FCM,and the dead rates of PC12 eels was higher in the 5.00×10~(-5)mol/L group than the control group(P<0.05).Immunonnores-cence eytochemistry experiment demonstrated that the level of Bcl-2 expression was significantly lower in the 3.13×10~(-6)mol/L group than the control group.Conclusion At low dosage,fipronil increases the apoptotic rates of PC12 cells possibly by decreasing the expression of Bcl-2 protein while at high dosage,fipmnil only in-creases the amount of necmtic cells.
