CAJ | 학술논문

目的探索低剂量天然放射性钍是否诱导出职业人员的外周血淋巴细胞适应性反应.方法选择包钢选矿厂含钍粉尘作业工人(暴露组)和对照人员各20例,采集外周静脉血,分析染色体畸变.同时,对采集的外周血用60Coγ射线离体照射2.0 Gy,分析非稳定性染色体畸变或用单细胞凝胶电泳分析DNA链断裂水平.结果照射前两组的双着丝粒畸变暴露组较高,照射2.0 Gy60Coγ射线后观察到的双着丝粒畸变暴露组低于对照组,但两组间差异无统计学意义(U=0.0026、0.0084,P>0.05),三着丝粒畸变在两组间差异具有统计学意义(u=3.1622,0.001<P<0.002);两组问的"彗星"尾长差异有统计学意义(t=25,P<0.001).结论低剂量天然放射性钍诱导出职业人员的外周血淋巴细胞染色体畸变和DNA损伤的适应性反应.
목적 탐색저제량천연방사성토시부유도출직업인원적외주혈림파세포괄응성반응.방법 선택포강선광엄함토분진작업공인(폭로조)화대조인원각20례,채집외주정맥혈,분석염색체기변.동시,대채집적외주혈용60Coγ사선리체조사2.0 Gy,분석비은정성염색체기변혹용단세포응효전영분석DNA련단렬수평.결과 조사전량조적쌍착사립기변폭로조교고,조사2.0 Gy60Coγ사선후관찰도적쌍착사립기변폭로조저우대조조,단량조간차이무통계학의의(U=0.0026、0.0084,P>0.05),삼착사립기변재량조간차이구유통계학의의(u=3.1622,0.001<P<0.002);량조문적"혜성"미장차이유통계학의의(t=25,P<0.001).결론 저제량천연방사성토유도출직업인원적외주혈림파세포염색체기변화DNA손상적괄응성반응.
Objective To explore if the occupational exposure to low dose thorium could induce adaptive response in peripheral lymphocytes.Methods 40 individuals.who exposed to thorium and rare earth mixed dust(exposure group) or control in Baotou Steel Plant, were selected, and chromosome aberrations were analyzed.Then the peripheral blood samples were irradiated in vitro with 2 Gy60Co γ-rays,and unstable chromosome aberration or DNA stand breakage analysis using single cell gel electrophoresis was performed. Results The dicentrics before 2 Gy exposure in exposure group was higher than that in control(P>0.05). But the dicentries after 2 Gy exposure in exposure group was lower than that in control,but not significantly (P>0.05).The tricentrics in exposure group was significantly lower than that in control(U=3.1622, 0.0ol<P<0.002).The DNA strand breakage in control group was significantly higher than that in exposure group(t=25,P<0.001).Conclusions Occupational exposure to low dose thorium could induce the adaptive response on chromosome aberration and DNA strand breakage in peripheral lymphocytes.