CAJ | 학술논문

目的用代谢组学方法研究大鼠胰腺组织代谢特征,以期发现胰腺炎症的标记性代谢物.方法 Wistar大鼠22只,按数字表法随机分成急性坏死性胰腺炎组(ANP,7只)、慢性胰腺炎组(CP,6只)和对照组(9只).ANP组经腹腔注射20％L-精氨酸溶液制模；CP组经尾静脉注射二丁基二氯基锡(DBTC)溶液制模；对照组注射等量生理盐水.检测血清淀粉酶含量,胰腺组织行病理学检查.利用高分辨魔角旋转核磁共振波谱对离体胰腺组织进行代谢成分分析,并对实验数据进行主成分分析,比较ANP与CP的代谢特征.结果与对照组相比,ANP组小分子代谢物亮氨酸、异亮氨酸、缬氨酸含量增加,而CP组这些代谢物含量减少.ANP组和CP组的磷酸胆碱、甘油磷酸胆碱、胆碱含量均升高,脂肪酸、乳酸、甜菜碱、甘氨酸含量均下降,CP组中脂类代谢产物含量明显高于ANP组,且仅在CP组中观察到牛磺酸含量升高.结论胰腺炎症疾病造成胰腺组织内代谢异常,升高的牛磺酸水平可能是区分CP和ANP的标记物.
목적 용대사조학방법연구대서이선조직대사특정,이기발현이선염증적표기성대사물.방법 Wistar대서22지,안수자표법수궤분성급성배사성이선염조(ANP,7지)、만성이선염조(CP,6지)화대조조(9지).ANP조경복강주사20％L-정안산용액제모；CP조경미정맥주사이정기이록기석(DBTC)용액제모；대조조주사등량생리염수.검측혈청정분매함량,이선조직행병이학검사.이용고분변마각선전핵자공진파보대리체이선조직진행대사성분분석,병대실험수거진행주성분분석,비교ANP여CP적대사특정.결과 여대조조상비,ANP조소분자대사물량안산、이량안산、힐안산함량증가,이CP조저사대사물함량감소.ANP조화CP조적린산담감、감유린산담감、담감함량균승고,지방산、유산、첨채감、감안산함량균하강,CP조중지류대사산물함량명현고우ANP조,차부재CP조중관찰도우광산함량승고.결론 이선염증질병조성이선조직내대사이상,승고적우광산수평가능시구분CP화ANP적표기물.
Objective To study the metabolite features of acute necrotizing pancreatitis (ANP) and chronic pancreatitis (CP) in rats.Methods A total of 22 Wistar rats were divided into ANP group (n =7 ),CP group (n =6) and the control group (n =9).ANP model was induced peritoneous injection of 20％ Larginine,and the rats were sacrificed 12 hours later.CP model was induced by intravenously injection of DBTC (8 mg/kg body weight),and the rats were sacrificed after 2 months.The rats in the control group received same amount of saline.Serum amylase was determined and pancreatic tissues were pathologically examined.Metabolic changes of pancreatic tissues in vitro were studied by high resolution magic angle spinning nuclear magnetic resonance (MAS NMR ),and analyzed by using principal components analysis (PCA).Characteristic metabolites of ANP and CP were compared. Results Compared with the control group,increased leucine,iso-leucine and valine levels were observed in ANP group,however,the opposite trends were observed in CP group.Phosphocholine,glycerophosphocholine,choline levels were increased and fatty acids,lactate,betaine,glycine levels were decreased in both ANP and CP groups.The lipid content in CP group were significantly higher than that in ANP group and the increased taurine was only observed in CP group. Conclusions There were obvious metabolic features in pancreatic tissue in rats with pancreatitis disorders,and the increased taurine could be used as biomarker to discriminate ANP and CP.