CAJ | 학술논문

目的了解湖北省经血途径感染HIV-1人群中合并HCV感染情况及HCV基因型分布.方法 2004年7月至2010年12月间在本院诊治或会诊的597例抗HIV阳性者进行HCV筛查,并行HCV病毒载量检测,对HCV RNA阳性者进行逆转录巢式聚合酶链反应扩增HCV核心基因区,并对扩增产物进行测序,采用Mega软件对所得序列进行基因树分析.结果既往有偿供血和受血的HIV感染者中HCV的感染率分别为76.5％(205/268),57.4％ (189/329).97例HIV、HCV合并感染者行HCV基因分型检测,发现1b型90例(92.8％)和2a型7例(7.2％),两型的HCV病毒载量(HCV-VL)差别无统计学意义[对数值分别为(6.0±1.0)拷贝/ml、(5.8±1.4)拷贝/ml,t=0.40,P=0.69].结论血液途径为HIV、HCV合并感染的主要途径.受血感染者合并HCV感染率低于献血人群,感染者中HCV的基因型主要为1b型和2a型.
목적 료해호북성경혈도경감염HIV-1인군중합병HCV감염정황급HCV기인형분포.방법 2004년7월지2010년12월간재본원진치혹회진적597례항HIV양성자진행HCV사사,병행HCV병독재량검측,대HCV RNA양성자진행역전록소식취합매련반응확증HCV핵심기인구,병대확증산물진행측서,채용Mega연건대소득서렬진행기인수분석.결과 기왕유상공혈화수혈적HIV감염자중HCV적감염솔분별위76.5％(205/268),57.4％ (189/329).97례HIV、HCV합병감염자행HCV기인분형검측,발현1b형90례(92.8％)화2a형7례(7.2％),량형적HCV병독재량(HCV-VL)차별무통계학의의[대수치분별위(6.0±1.0)고패/ml、(5.8±1.4)고패/ml,t=0.40,P=0.69].결론 혈액도경위HIV、HCV합병감염적주요도경.수혈감염자합병HCV감염솔저우헌혈인군,감염자중HCV적기인형주요위1b형화2a형.
Objective To explore the epidemiologic features and distribution pattems of hepatitis C virus (HCV) genotype infection among HIV positive former blood donors (FBDs) and transfusion recipients in Hubei province.Methods 597 serum samples from HIV-positive patients in Hubei were collected and examined for anti-HCV by enzyme-linked immunosorbent assay ( ELISA ).Reverse transcription nested polymerase chain reaction (RT-nested PCR) amplification and DNA sequencing were used to evaluate the HCV core regions.Results The prevalence rates of HCV in HIV positive FBDs and transfusion recipients were 76.5％ (205/268) and 57.4％ (189/329) respectively.HCV genotypes 1b (92.8％,90/97) and 2a (7.2％,7/97 ) were detected.Conclusions Blood donation and blood transfusion are the major modes of HIV-HCV co-infection in Hubei province.The prevalence of HCV in HIV positive transfusion recipients is lower than that in HIV positive FBDs.HCV genotypes 1b and 2a are the predominant strains among HIV-positive FBDs and transfusion recipients.