中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2012年
3期
242-245
,共4页
朱晓峰%戚询中%时晶%孙光涛%田金洲
硃曉峰%慼詢中%時晶%孫光濤%田金洲
주효봉%척순중%시정%손광도%전금주
神经干细胞%β-淀粉样蛋白%远志总皂苷%扫描电镜
神經榦細胞%β-澱粉樣蛋白%遠誌總皂苷%掃描電鏡
신경간세포%β-정분양단백%원지총조감%소묘전경
Neural stem cell%β-amyloid protein%Tenuigenin%Scanning electron microscope
目的 研究远志总皂苷(TEN)对Aβ致伤小鼠海马神经干细胞(NSCs)突起损伤的保护作用. 方法 取新生24h内昆明小鼠海马NSCs,传代3次后采用Aβ致伤及TEN干预.细胞共分5组:对照组、Aβ1-42组、TEN5mg/L组、TEN 20 mg/L组、TEN 100 mg/L组,对照组用含2％B27的DMEM液培养；其余4组加入Aβ1-42,使其终浓度为12.5 μmol/L;TEN 5 mg/L组、TEN 20mg/L组、TEN 100 mg/L组在此基础上再加入TEN,调定终浓度为5 mg/L、20 mg/L、100 mg/L.干预3d后扫描电镜观察各组细胞,同时应用光学显微镜测量细胞突起长度及细胞突起个数. 结果 与对照组相比,Aβ1-42组NSCs突起数量减少,长度缩短,差异有统计学意义(P＜0.05).与Aβ1-42组相比,20 mg/L、100 mg/L TEN组突起数量明显增多,突起长度明显增加,差异均有统计学意义(P＜0.05). 结论 TEN能够抑制Aβ1-42所致NSCs突起结构损伤,对NSCs具有保护作用.
目的 研究遠誌總皂苷(TEN)對Aβ緻傷小鼠海馬神經榦細胞(NSCs)突起損傷的保護作用. 方法 取新生24h內昆明小鼠海馬NSCs,傳代3次後採用Aβ緻傷及TEN榦預.細胞共分5組:對照組、Aβ1-42組、TEN5mg/L組、TEN 20 mg/L組、TEN 100 mg/L組,對照組用含2％B27的DMEM液培養；其餘4組加入Aβ1-42,使其終濃度為12.5 μmol/L;TEN 5 mg/L組、TEN 20mg/L組、TEN 100 mg/L組在此基礎上再加入TEN,調定終濃度為5 mg/L、20 mg/L、100 mg/L.榦預3d後掃描電鏡觀察各組細胞,同時應用光學顯微鏡測量細胞突起長度及細胞突起箇數. 結果 與對照組相比,Aβ1-42組NSCs突起數量減少,長度縮短,差異有統計學意義(P＜0.05).與Aβ1-42組相比,20 mg/L、100 mg/L TEN組突起數量明顯增多,突起長度明顯增加,差異均有統計學意義(P＜0.05). 結論 TEN能夠抑製Aβ1-42所緻NSCs突起結構損傷,對NSCs具有保護作用.
목적 연구원지총조감(TEN)대Aβ치상소서해마신경간세포(NSCs)돌기손상적보호작용. 방법 취신생24h내곤명소서해마NSCs,전대3차후채용Aβ치상급TEN간예.세포공분5조:대조조、Aβ1-42조、TEN5mg/L조、TEN 20 mg/L조、TEN 100 mg/L조,대조조용함2％B27적DMEM액배양；기여4조가입Aβ1-42,사기종농도위12.5 μmol/L;TEN 5 mg/L조、TEN 20mg/L조、TEN 100 mg/L조재차기출상재가입TEN,조정종농도위5 mg/L、20 mg/L、100 mg/L.간예3d후소묘전경관찰각조세포,동시응용광학현미경측량세포돌기장도급세포돌기개수. 결과 여대조조상비,Aβ1-42조NSCs돌기수량감소,장도축단,차이유통계학의의(P＜0.05).여Aβ1-42조상비,20 mg/L、100 mg/L TEN조돌기수량명현증다,돌기장도명현증가,차이균유통계학의의(P＜0.05). 결론 TEN능구억제Aβ1-42소치NSCs돌기결구손상,대NSCs구유보호작용.
Objective To study the effect oftenuigenin (TEN) on the processes of neural stem cells (NSCs) injured by β-amyloid (Aβ) protein. Methods Mouse NSCs were generated from the hippocampi of Kunming mice within 24 hour from birth and cultured with epidermal growth factor (EGF)and basic fibroblast growth factor (bFGF) (20 ng/mL each) in 50-mm uncoated culture flasks.The third passage NSCs were cultured in Aβ medium (12.5 μmol/L) and TEN medium (5 mg/L,20 mg/L,100mg/L) respectively and observed under a scanning electron microscope (SEM) after 3 days.Optical microscopy was used to detect the length and amount of the processes of NSCs. The statistical significance between group comparisons was determined by t test.P value ＜0.05 was considered to be statistically significant. Results The length and amount of NSC processes in Aβ1-42 group were both significantly shorter and smaller than in the control group (P＜0.05). The length and amount of NSC processes in 20 mg/L and 100 mg/L groups were both significantly longer and larger than in the Aβ-42group (P＜0.05). Conclusion TEN can significantly increase the length and amount of NSC processes injured by Aβ.
