中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2011年
5期
331-333
,共3页
马佰菁%黄菱%韩梅%王玉巧%赵建宁%孙伯坚%林佳静%宋婷阁
馬佰菁%黃蔆%韓梅%王玉巧%趙建寧%孫伯堅%林佳靜%宋婷閣
마백정%황릉%한매%왕옥교%조건저%손백견%림가정%송정각
胃肿瘤%赖氨酰氧化酶%细胞迁移%细胞黏附%肿瘤转移
胃腫瘤%賴氨酰氧化酶%細胞遷移%細胞黏附%腫瘤轉移
위종류%뢰안선양화매%세포천이%세포점부%종류전이
Stomach neoplasms%Lysyl oxidase%Cell invasion%Cell adhesion%Neoplasms metastasis
目的 研究赖氨酰氧化酶(LOX)对胃癌细胞HGC-27体外迁移和黏附能力的影响.方法 体外培养胃癌细胞HGC-27,以不同浓度的B-氨基丙腈(BAPN)抑制LOX的活性.采用体外划痕实验检测对细胞迁移能力的影响,采用同质黏附和异质黏附实验分析对细胞黏附能力的影响.结果 当BAPN浓度为0.2 mmol/L时,胃癌细胞的体外迁移能力在8、24、32、48 h各时间点均较未加BAPN下降(P<0.05);且HGC-2q细胞在同质黏附介质上生长60 ndn后,同质黏附细胞数为(7.78±0.11)×103个/ml,与对照组[(6.97±O.07)×103个/ml]比较,差异有统计学意义(P<0.05);异质黏附细胞数为(8.35±0.10)×103个/ml,与对照组[(8.98±0.15)×103个/ml]比较,差异有统计学意义(P<0.05).当BAPN浓度为0.3 mmol/L时,胃癌细胞的体外迁移能力在各时间点均较未加BAPN下降,同质黏附细胞数增加至(8.02±0.11)×103个/ml,异质黏附细胞数减少至(7.93±0.07)×103个/ml,与对照组或0.2 mmol/L BAPN组比较,差异均有统计学意义(P<0.05).结论 LOX可能通过促进肿瘤细胞的迁移和异质黏附能力,降低其同质黏附能力,促进肿瘤的转移.
目的 研究賴氨酰氧化酶(LOX)對胃癌細胞HGC-27體外遷移和黏附能力的影響.方法 體外培養胃癌細胞HGC-27,以不同濃度的B-氨基丙腈(BAPN)抑製LOX的活性.採用體外劃痕實驗檢測對細胞遷移能力的影響,採用同質黏附和異質黏附實驗分析對細胞黏附能力的影響.結果 噹BAPN濃度為0.2 mmol/L時,胃癌細胞的體外遷移能力在8、24、32、48 h各時間點均較未加BAPN下降(P<0.05);且HGC-2q細胞在同質黏附介質上生長60 ndn後,同質黏附細胞數為(7.78±0.11)×103箇/ml,與對照組[(6.97±O.07)×103箇/ml]比較,差異有統計學意義(P<0.05);異質黏附細胞數為(8.35±0.10)×103箇/ml,與對照組[(8.98±0.15)×103箇/ml]比較,差異有統計學意義(P<0.05).噹BAPN濃度為0.3 mmol/L時,胃癌細胞的體外遷移能力在各時間點均較未加BAPN下降,同質黏附細胞數增加至(8.02±0.11)×103箇/ml,異質黏附細胞數減少至(7.93±0.07)×103箇/ml,與對照組或0.2 mmol/L BAPN組比較,差異均有統計學意義(P<0.05).結論 LOX可能通過促進腫瘤細胞的遷移和異質黏附能力,降低其同質黏附能力,促進腫瘤的轉移.
목적 연구뢰안선양화매(LOX)대위암세포HGC-27체외천이화점부능력적영향.방법 체외배양위암세포HGC-27,이불동농도적B-안기병정(BAPN)억제LOX적활성.채용체외화흔실험검측대세포천이능력적영향,채용동질점부화이질점부실험분석대세포점부능력적영향.결과 당BAPN농도위0.2 mmol/L시,위암세포적체외천이능력재8、24、32、48 h각시간점균교미가BAPN하강(P<0.05);차HGC-2q세포재동질점부개질상생장60 ndn후,동질점부세포수위(7.78±0.11)×103개/ml,여대조조[(6.97±O.07)×103개/ml]비교,차이유통계학의의(P<0.05);이질점부세포수위(8.35±0.10)×103개/ml,여대조조[(8.98±0.15)×103개/ml]비교,차이유통계학의의(P<0.05).당BAPN농도위0.3 mmol/L시,위암세포적체외천이능력재각시간점균교미가BAPN하강,동질점부세포수증가지(8.02±0.11)×103개/ml,이질점부세포수감소지(7.93±0.07)×103개/ml,여대조조혹0.2 mmol/L BAPN조비교,차이균유통계학의의(P<0.05).결론 LOX가능통과촉진종류세포적천이화이질점부능력,강저기동질점부능력,촉진종류적전이.
Objective To study the effects of lysyl oxidase ( LOX) on the migration and adhesion of the human gastric cancer cell line HGC-27 cells in vitro. Methods The human gastric cancer cell line HGC-27 cells were cultured in vitro, and treated with different concentration of β-aminopropionitrile (BAPN). The ability of migration was assessed by wound-healing assay. The ability of adhesion was detected by homogenous and heterogeneous adhesion experiments. Results Compared that with 0 mmol/L BAPN, the ability of migration of the cells after treatment with 0.2 mmol/L BAPN was descended at 8, 24, 32 and 48 h; the number of cells with homogeneous adhesion was increased from (6.97 ± 0.07) x l03/ml to (7.78±0.11) ×103/ml; and the number of cells with heterogeneous adhesion was decreased from (8.98± 0. 15) x 103/ml to (8. 35 ±0. 10) ×103/ml, both <0. 05. Compared with that of cells treated with 0 mmol/L and 0.2 mmol/L BAPN, the migration ability of cells after treatment with 0.3 mmol/L BAPN was descended at 8, 24, 32 and 48 h; the number of cells with homogeneous adhesion was raised to (8. 02 ± 0. 11) ×103/ml and the number of cells with heterogeneous adhesion was down to (7.93 ±0.07) ×103/ml (P < 0. 05). Conclusion LOX may promote the metastasis of cancer cells by enhancing invasion, increasing heterogeneous adhesion and decreasing homogeneous adhesion.
