CAJ | 학술논문

目的分析我国荒漠、山丘疫区利什曼原虫分离株的 SSU rDNA多变区序列差异。方法 nDNA进行PCR扩增，将扩增出的SSU rDNA基因的特异片段克隆于pGEMR-T Easy Vector上，采用通用引物M13进行PCR扩增，全自动测序仪测序。结果序列分析显示本文报道的荒漠、山丘疫区的2株利什曼原虫（L.d.XJ771、L.d.SC6）的SSU rDNA序列大小均为392bp；序列差异发生在两个独特序列区（UQ-Ⅰ和UQ-Ⅱ），无移码突变；与GeneBank中的利什曼原虫比较分析，同源性在98%以上。结论我国荒漠、山丘疫区利什曼原虫分离株之间的SSU rDNA多变区的碱基序列有差异；荒漠疫区分离株L.d.XJ771与国际标准株L.d. DD8的SSU rDNA多变区的碱基序列完全相同。
목적분석아국황막、산구역구리십만원충분리주적 SSU rDNA다변구서렬차이。방법 nDNA진행PCR확증，장확증출적SSU rDNA기인적특이편단극륭우pGEMR-T Easy Vector상，채용통용인물M13진행PCR확증，전자동측서의측서。결과서렬분석현시본문보도적황막、산구역구적2주리십만원충（L.d.XJ771、L.d.SC6）적SSU rDNA서렬대소균위392bp；서렬차이발생재량개독특서렬구（UQ-Ⅰ화UQ-Ⅱ），무이마돌변；여GeneBank중적리십만원충비교분석，동원성재98%이상。결론아국황막、산구역구리십만원충분리주지간적SSU rDNA다변구적감기서렬유차이；황막역구분리주L.d.XJ771여국제표준주L.d. DD8적SSU rDNA다변구적감기서렬완전상동。
Aim　 To analyze the sequence of the SSU rDNA variable region of Leishmania isolates from desert and hill foci of China. Methods　 Specific SSU rDNA fragments from nuclear DNA of two Leishmania isolates were amplified by PCR and then cloned into pGEMR-T Easy Vector.After that, the specific fragments were sequenced by the automated DNA sequencer. Results　Sequence analysis showed that the amplified DNA fragments of two Leishmania isolates(L.d.XJ771 and L.d.SC6) were all 392bp in length, point mutations were located in the two unique sequence (UQ-Ⅰ and UQ-Ⅱ),and no insertion/deletion found. The identities of comparison of Leishmania in GeneBank were more than 98%.Conclusion　Sequence difference of the SSU rDNA variable region existed among Leishmania isolates from desert and hill foci; The sequences of the SSU rDNA variable region of L.d.XJ771 isolate and L. d.DD8 were identical.