上海农业学报
上海農業學報
상해농업학보
ACTA AGRICULTURAE SHANGHAI
2001年
1期
70-73
,共4页
模拟生产%发酵工艺%培养基
模擬生產%髮酵工藝%培養基
모의생산%발효공예%배양기
90B4-2-2菌株的培养及最大菌落数产生的发酵模拟生产工艺条件，正交试验得到最适培养基碳、氮源组成为（%）:黄豆饼粉1.0,淀粉1.5,酵母粉0.8;最适生长温度为30℃；pH7.0,接种量4%,在250mL三角瓶中装70mL培养基,200r/min摇床上培养35h,菌落数为6.69×109CFU/mL;上罐(1～2t)发酵22 h，终点总的菌落数为(1.85～2.50)×1010CFU/mL。
90B4-2-2菌株的培養及最大菌落數產生的髮酵模擬生產工藝條件，正交試驗得到最適培養基碳、氮源組成為（%）:黃豆餅粉1.0,澱粉1.5,酵母粉0.8;最適生長溫度為30℃；pH7.0,接種量4%,在250mL三角瓶中裝70mL培養基,200r/min搖床上培養35h,菌落數為6.69×109CFU/mL;上罐(1～2t)髮酵22 h，終點總的菌落數為(1.85～2.50)×1010CFU/mL。
90B4-2-2균주적배양급최대균락수산생적발효모의생산공예조건，정교시험득도최괄배양기탄、담원조성위（%）:황두병분1.0,정분1.5,효모분0.8;최괄생장온도위30℃；pH7.0,접충량4%,재250mL삼각병중장70mL배양기,200r/min요상상배양35h,균락수위6.69×109CFU/mL;상관(1～2t)발효22 h，종점총적균락수위(1.85～2.50)×1010CFU/mL。
The simulated production conditions of bacterialwilt-antagonistic strain 90B4-2-2 were studied through culture with different steps and orthogonal test of different medium compositions. The results are summarized as follows. The optimum medium composition is (%): soy bean meal(1.0)+starch(1.5)+yeast powder(0.8). The optimum growth temperature is 30℃. When the strain is cultured for 35 hours on a 200r/min shaker with 70mL medium in 250mL flask at 7.0pH and 4% inoculation level, the total bacterial count is 6.69×109CFU/mL. When the fermentation is done in 1～2 ton fermenters, the final total bacterial counts are 1.85～2.50×1010CFU/mL.
