西安医科大学学报
西安醫科大學學報
서안의과대학학보
JOURNAL OF XI'AN MEDICAL UNIVERSITY
2001年
1期
7-10
,共4页
针灸%巨噬细胞%白细胞介素-1%辅助性T细胞%荷瘤小鼠
針灸%巨噬細胞%白細胞介素-1%輔助性T細胞%荷瘤小鼠
침구%거서세포%백세포개소-1%보조성T세포%하류소서
目的 以荷瘤(S37)小鼠为模型，进一步验证针灸并施的抗肿瘤和对IL2-IFN-NKC调节网的相关网络MΦ-IL1-Th的效应。方法 针灸荷瘤(S37)小鼠的大椎、后海二穴后，取样，以吞噬中性红比色分析法，MTT比色分析法和单抗SPA-Ig花环法等观察针灸对MΦ、IL1、Th及S37肉瘤的影响。结果 荷瘤针灸并施组、荷瘤艾灸组、荷瘤针刺组的小鼠腹腔MΦ吞噬功能、IL1含量及Th细胞(L3T4)百分率均较荷瘤对照组显著增高；除针刺组外，其余两实验组均对S37肉瘤有明显抑制作用。另外，针灸组和艾灸组IL1含量均较针刺组显著增高；针灸组Th细胞(L3T4）较针刺组显著增高。结论 针灸荷瘤小鼠大椎、后海二穴，具有正向调节IL2-IFN-NKC网的相关网络MΦ-IL1-Th和抑瘤(S37)作用。艾灸也显示出相似的效果。
目的 以荷瘤(S37)小鼠為模型，進一步驗證針灸併施的抗腫瘤和對IL2-IFN-NKC調節網的相關網絡MΦ-IL1-Th的效應。方法 針灸荷瘤(S37)小鼠的大椎、後海二穴後，取樣，以吞噬中性紅比色分析法，MTT比色分析法和單抗SPA-Ig花環法等觀察針灸對MΦ、IL1、Th及S37肉瘤的影響。結果 荷瘤針灸併施組、荷瘤艾灸組、荷瘤針刺組的小鼠腹腔MΦ吞噬功能、IL1含量及Th細胞(L3T4)百分率均較荷瘤對照組顯著增高；除針刺組外，其餘兩實驗組均對S37肉瘤有明顯抑製作用。另外，針灸組和艾灸組IL1含量均較針刺組顯著增高；針灸組Th細胞(L3T4）較針刺組顯著增高。結論 針灸荷瘤小鼠大椎、後海二穴，具有正嚮調節IL2-IFN-NKC網的相關網絡MΦ-IL1-Th和抑瘤(S37)作用。艾灸也顯示齣相似的效果。
목적 이하류(S37)소서위모형，진일보험증침구병시적항종류화대IL2-IFN-NKC조절망적상관망락MΦ-IL1-Th적효응。방법 침구하류(S37)소서적대추、후해이혈후，취양，이탄서중성홍비색분석법，MTT비색분석법화단항SPA-Ig화배법등관찰침구대MΦ、IL1、Th급S37육류적영향。결과 하류침구병시조、하류애구조、하류침자조적소서복강MΦ탄서공능、IL1함량급Th세포(L3T4)백분솔균교하류대조조현저증고；제침자조외，기여량실험조균대S37육류유명현억제작용。령외，침구조화애구조IL1함량균교침자조현저증고；침구조Th세포(L3T4）교침자조현저증고。결론 침구하류소서대추、후해이혈，구유정향조절IL2-IFN-NKC망적상관망락MΦ-IL1-Th화억류(S37)작용。애구야현시출상사적효과。
Objective The tumor-bearing (S37) mice were used to study theeffects of acupuncture and moxibustion on relative network of IL2-IFN-NKC regulatory network.Methods After puncturing and (or) igniting the acupoints of Dazhui(Du 14) and Houhai(Du 1), we observed its effects on MΦ,IL1,Th and tumor S37 by means of MΦ devouring neutral red test,MTT colorimetry and Anti-L3T4 McAb-SPA rosetle test simultaneously.Results The phagocytic power of MΦ in the mices abdomen,the amount of IL1 and percentage of L3T4 increased obviously,compared to that of the control group;excepted the acupuncture group,the other two experimental groups had the obvious inhibition to the tumor S37. In addition,the IL1 content increased remarkably in acumoxi group and moxibustion group than in acupuncture group. The percentage of L3T4 increased remarkably in acumoxi group than that in acupuncture group.Conclusion Applying both acupuncture and moxibustion at points of Dazhui(Du 14) and Houhai(Du 1) can make positive regulation on the relative MΦ-IL1-Th network of IL2-IFN-NKC regulatory network and has anti-tumor effect.The moxibustion also has the similar effects.
